Restriction map for
pF1KB5462
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
HaeII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 565 GACGTC GACGT^C
AccI 277 GTCTAC GT^[AC][GT]AC
AccII 562 CGCG CG^CG
AccIII 641 TCCGGA T^CCGGA
AcyI 565 GACGTC G[GA]^CG[CT]C
AflIII 722 ACACGT A^C[GA][CT]GT
AlwI 288 GGATC GGATC(4/5)
ApaI 770 GGGCCC GGGCC^C
AspI 484 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 593 TGCGCA TGC^GCA
BcgI 125 CGAAGCACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 472 CTAG C^TAG
BglII 986 AGATCT A^GATCT
BsaHI 565 GACGTC G[GA]^CG[CT]C
BsaWI 641 TCCGGA [AT]^CCGG[AT]
BseAI 641 TCCGGA T^CCGGA
BspEI 641 TCCGGA T^CCGGA
BspMI 866 ACCTGC ACCTGC(4/8)
BsrBI 340 CCGCTC CCGCTC(-3/-3)
BsrDI 552 CATTGC GCAATG(2/0)
BsrI 245 ACTGG ACTGG(1/-1)
BstUI 562 CGCG CG^CG
BstYI 986 AGATCT [GA]^GATC[CT]
DraIII 229 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 221 CCATGG C^C[GA][CT]GG
EcoNI 327 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 973 GATATC GAT^ATC
FspI 593 TGCGCA TGC^GCA
Hin1I 565 GACGTC G[GA]^CG[CT]C
HincII 607 GTTGAC GT[CT]^[GA]AC
HindII 607 GTTGAC GT[CT]^[GA]AC
HindIII 958 AAGCTT A^AGCTT
MaeI 472 CTAG C^TAG
MaeIII 490 GTCAC ^GT[ACGT]AC
MflI 986 AGATCT [GA]^GATC[CT]
MroI 641 TCCGGA T^CCGGA
MspA1I 292 CAGCTG C[AC]G^C[GT]G
MvnI 562 CGCG CG^CG
NcoI 221 CCATGG C^CATGG
NspI 131 ACATGC [GA]CATG^[CT]
PflFI 484 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 161 GGGACCT [GA]G^G[AT]CC[CT]
PstI 295 CTGCAG CTGCA^G
PvuII 292 CAGCTG CAG^CTG
SfcI 295 CTGCAG C^T[GA][CT]AG
TaqI 41 TCGA T^CGA
Tsp45I 490 GTCAC ^GT[GC]AC
TspRI 945 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 484 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 41 TCGA T^CGA
XbaI 471 TCTAGA T^CTAGA
XhoII 986 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AvaI 348 CCCGAG C^[CT]CG[GA]G
775 CCCGAG
BsgI 758 CTGCAC GTGCAG(16/14)
715 GTGCAG
BsiEI 121 CGGCCG CG[GA][CT]^CG
558 CGGCCG
BsmBI 567 CGTCTC CGTCTC(1/5)
703 GAGACG
BsmFI 161 GGGAC GGGAC(10/14)
482 GGGAC
BsoBI 348 CCCGAG C^[CT]CG[GA]G
775 CCCGAG
BsrFI 402 GCCGGC [GA]^CCGG[CT]
556 GCCGGC
BstAPI 315 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
423 GCAGGCTGTGC
BstNI 153 CCTGG CC^[AT]GG
1038 CCTGG
CfoI 594 GCGC GCG^C
870 GCGC
Cfr10I 402 GCCGGC [GA]^CCGG[CT]
556 GCCGGC
DpnII 289 GATC ^GATC
987 GATC
EaeI 121 CGGCCG [CT]^GGCC[GA]
558 CGGCCG
EagI 121 CGGCCG C^GGCCG
558 CGGCCG
EarI 166 CTCTTC CTCTTC(1/4)
1090 GAAGAG
EclXI 121 CGGCCG C^GGCCG
558 CGGCCG
Eco52I 121 CGGCCG C^GGCCG
558 CGGCCG
EcoRII 153 CCTGG ^CC[AT]GG
1038 CCTGG
EcoT14I 221 CCATGG C^C[AT][AT]GG
284 CCAAGG
HgaI 452 GACGC GACGC(5/10)
876 GACGC
HhaI 594 GCGC GCG^C
870 GCGC
HinP1I 594 GCGC G^CGC
870 GCGC
HphI 57 GGTGA GGTGA(8/7)
138 GGTGA
Ksp632I 166 CTCTTC CTCTTC(1/4)
1090 GAAGAG
MboI 289 GATC ^GATC
987 GATC
MseI 62 TTAA T^TAA
1058 TTAA
MvaI 153 CCTGG CC^[AT]GG
1038 CCTGG
NaeI 402 GCCGGC GCC^GGC
556 GCCGGC
NdeII 289 GATC ^GATC
987 GATC
NgoMIV 402 GCCGGC G^CCGGC
556 GCCGGC
PflMI 27 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCATGAGGTGG
Sau3AI 289 GATC ^GATC
987 GATC
SfaNI 263 GCATC GCATC(5/9)
315 GCATC
StyI 221 CCATGG C^C[AT][AT]GG
284 CCAAGG
TfiI 87 GATTC G^A[AT]TC
764 GATTC
Tru9I 62 TTAA T^TAA
1058 TTAA
Van91I 27 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCATGAGGTGG
XcmI 23 CCAACCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
536 CCATCATGCGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
Cac8I Cfr13I DdeI DraII EcoO109I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeII MboII
MnlI MslI MspI MwoI NciI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI TseI