Restriction map for
pF1KE2764
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BbeI BbvCI BciVI BfrI BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 955 GAATTC [GA]^AATT[CT]
AcyI 114 GGCGTC G[GA]^CG[CT]C
AlwNI 838 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 541 CCTCAGG CC^T[ACGT]AGG
ApoI 955 GAATTC [GA]^AATT[CT]
BamHI 1410 GGATCC G^GATCC
BbrPI 1195 CACGTG CAC^GTG
BcgI 1535 GCAATGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1353 TGATCA T^GATCA
BcnI 51 CCCGG CC^[GC]GG
BglII 788 AGATCT A^GATCT
BsaBI 1386 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 114 GGCGTC G[GA]^CG[CT]C
BsiWI 1088 CGTACG C^GTACG
BsrFI 46 GCCGGC [GA]^CCGG[CT]
BssSI 913 CACGAG CACGAG(-5/-1)
BstAPI 138 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 972 GGTGACC G^GT[ACGT]ACC
BstPI 972 GGTGACC G^GT[ACGT]ACC
Bsu36I 541 CCTCAGG CC^T[ACGT]AGG
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
Eco81I 541 CCTCAGG CC^T[ACGT]AGG
EcoO65I 972 GGTGACC G^GT[ACGT]ACC
EcoRI 955 GAATTC G^AATTC
FbaI 1353 TGATCA T^GATCA
Hin1I 114 GGCGTC G[GA]^CG[CT]C
MamI 1386 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 603 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 46 GCCGGC GCC^GGC
NciI 51 CCCGG CC^[GC]GG
NgoMIV 46 GCCGGC G^CCGGC
PmaCI 1195 CACGTG CAC^GTG
PmlI 1195 CACGTG CAC^GTG
PpuMI 917 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 840 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI 1306 CTTGAG C^T[CT][GA]AG
SphI 1148 GCATGC GCATG^C
StuI 659 AGGCCT AGG^CCT
TfiI 444 GAATC G^A[AT]TC
XcmI 833 CCATGCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 36 CGCG CG^CG
1172 CGCG
ApaI 699 GGGCCC GGGCC^C
1491 GGGCCC
AspHI 393 GTGCTC G[AT]GC[AT]^C
1035 GTGCTC
AspI 111 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
202 GACGTTGTC
BalI 20 TGGCCA TGG^CCA
515 TGGCCA
BanI 95 GGTGCC G^G[CT][GA]CC
1505 GGTGCC
BbsI 108 GAAGAC GAAGAC(2/6)
273 GAAGAC
BfaI 43 CTAG C^TAG
433 CTAG
BglI 26 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
853 GCCAAAGTGGC
BpuAI 108 GAAGAC GAAGAC(2/6)
273 GAAGAC
BsaAI 1086 CACGTA [CT]AC^GT[GA]
1195 CACGTG
BsiHKAI 393 GTGCTC G[AT]GC[AT]^C
1035 GTGCTC
BsmAI 1519 GAGAC GTCTC(1/5)
417 GTCTC
BsmI 70 GAATGC GAATGC(1/-1)
602 GCATTC
BspLU11I 302 ACATGT A^CATGT
672 ACATGT
BsrDI 1549 CATTGC GCAATG(2/0)
1535 GCAATG
BstUI 36 CGCG CG^CG
1172 CGCG
CfoI 35 GCGC GCG^C
796 GCGC
EarI 10 CTCTTC CTCTTC(1/4)
1063 GAAGAG
HapII 47 CCGG C^CGG
52 CCGG
HgaI 1018 GACGC GACGC(5/10)
115 GCGTC
HhaI 35 GCGC GCG^C
796 GCGC
HinP1I 35 GCGC G^CGC
796 GCGC
HpaII 47 CCGG C^CGG
52 CCGG
Ksp632I 10 CTCTTC CTCTTC(1/4)
1063 GAAGAG
MaeI 43 CTAG C^TAG
433 CTAG
MluNI 20 TGGCCA TGG^CCA
515 TGGCCA
MscI 20 TGGCCA TGG^CCA
515 TGGCCA
MseI 362 TTAA T^TAA
905 TTAA
MspA1I 215 CAGCTG C[AC]G^C[GT]G
457 CAGCTG
MspI 47 CCGG C^CGG
52 CCGG
MvnI 36 CGCG CG^CG
1172 CGCG
NdeI 192 CATATG CA^TATG
717 CATATG
PflFI 111 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
202 GACGTTGTC
PflMI 657 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1071 CCAGGAGGTGG
PstI 145 CTGCAG CTGCA^G
1317 CTGCAG
PvuII 215 CAGCTG CAG^CTG
457 CAGCTG
SfaNI 768 GATGC GCATC(5/9)
828 GATGC
SfcI 145 CTGCAG C^T[GA][CT]AG
1317 CTGCAG
Tru9I 362 TTAA T^TAA
905 TTAA
Tth111I 111 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
202 GACGTTGTC
Van91I 657 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1071 CCAGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BanII BbvI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII