Restriction map for
pF1KB7613
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AccII AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsiEI BsiWI
BsmAI BsmBI BsmI BspDI BspHI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HincII HindII
HindIII HpaI KasI KspI MamI
MluI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 226 GACGTC GACGT^C
AccI 286 GTAGAC GT^[AC][GT]AC
AccIII 903 TCCGGA T^CCGGA
AciI 762 CCGC CCGC(-3/-1)
AcyI 226 GACGTC G[GA]^CG[CT]C
AflIII 587 ACATGT A^C[GA][CT]GT
AocI 297 CCTAAGG CC^T[ACGT]AGG
ApaI 757 GGGCCC GGGCC^C
Asp700I 550 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 716 CTCGAG C^[CT]CG[GA]G
AvaII 294 GGACC G^G[AT]CC
BciVI 724 GTATCC GTATCC(6/5)
BcnI 252 CCGGG CC^[GC]GG
BfaI 382 CTAG C^TAG
BglII 417 AGATCT A^GATCT
BsaHI 226 GACGTC G[GA]^CG[CT]C
BsaWI 903 TCCGGA [AT]^CCGG[AT]
BseAI 903 TCCGGA T^CCGGA
BsgI 769 GTGCAG GTGCAG(16/14)
BsoBI 716 CTCGAG C^[CT]CG[GA]G
Bsp1407I 59 TGTACA T^GTACA
BspEI 903 TCCGGA T^CCGGA
BspLU11I 587 ACATGT A^CATGT
BsrGI 59 TGTACA T^GTACA
BstEII 264 GGTAACC G^GT[ACGT]ACC
BstPI 264 GGTAACC G^GT[ACGT]ACC
Bsu36I 297 CCTAAGG CC^T[ACGT]AGG
CfoI 363 GCGC GCG^C
DraIII 88 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 637 CTCTTC CTCTTC(1/4)
Eco81I 297 CCTAAGG CC^T[ACGT]AGG
EcoO65I 264 GGTAACC G^GT[ACGT]ACC
FauI 761 CCCGC CCCGC(4/6)
HhaI 363 GCGC GCG^C
Hin1I 226 GACGTC G[GA]^CG[CT]C
HinP1I 363 GCGC G^CGC
Ksp632I 637 CTCTTC CTCTTC(1/4)
MaeI 382 CTAG C^TAG
MaeII 227 ACGT A^CGT
MfeI 399 CAATTG C^AATTG
MroI 903 TCCGGA T^CCGGA
MseI 833 TTAA T^TAA
MspA1I 808 CAGCTG C[AC]G^C[GT]G
MunI 399 CAATTG C^AATTG
NciI 252 CCGGG CC^[GC]GG
NdeI 569 CATATG CA^TATG
PaeR7I 716 CTCGAG C^TCGAG
PflMI 87 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 293 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 808 CAGCTG CAG^CTG
SspBI 59 TGTACA T^GTACA
Tru9I 833 TTAA T^TAA
Tsp45I 442 GTGAC ^GT[GC]AC
Van91I 87 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 700 CCAGAGGATGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 716 CTCGAG C^TCGAG
XmnI 550 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 536 GGTACC G^GTACC
780 GGTACC
AlwI 73 GATCC GGATC(4/5)
354 GGATC
AlwNI 23 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
808 CAGCTGCTG
Asp718I 536 GGTACC G^GTACC
780 GGTACC
BalI 84 TGGCCA TGG^CCA
482 TGGCCA
BpmI 698 CTCCAG CTGGAG(16/14)
187 CTGGAG
BsmFI 52 GGGAC GGGAC(10/14)
115 GTCCC
BstYI 354 GGATCT [GA]^GATC[CT]
417 AGATCT
DsaI 518 CCGTGG C^C[GA][CT]GG
735 CCGTGG
EaeI 84 TGGCCA [CT]^GGCC[GA]
482 TGGCCA
HphI 441 GGTGA GGTGA(8/7)
411 TCACC
KpnI 536 GGTACC GGTAC^C
780 GGTACC
MboII 555 TCTTC GAAGA(8/7)
638 TCTTC
MflI 354 GGATCT [GA]^GATC[CT]
417 AGATCT
MluNI 84 TGGCCA TGG^CCA
482 TGGCCA
MscI 84 TGGCCA TGG^CCA
482 TGGCCA
MwoI 816 GCTGGAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
856 GCCTTTCGAGC
NspI 282 GCATGT [GA]CATG^[CT]
587 ACATGT
PleI 113 GAGTC GAGTC(4/5)
796 GAGTC
PstI 559 CTGCAG CTGCA^G
868 CTGCAG
SacI 542 GAGCTC GAGCT^C
863 GAGCTC
SmlI 100 CTCAAG C^T[CT][GA]AG
716 CTCGAG
StuI 141 AGGCCT AGG^CCT
378 AGGCCT
TfiI 488 GAATC G^A[AT]TC
848 GAATC
Tsp509I 33 AATT ^AATT
400 AATT
XhoII 354 GGATCT [GA]^GATC[CT]
417 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AfaI AluI AspHI BanI BanII
BbvI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeIII MboI MnlI MslI
MspI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI TspRI TthHB8I