Restriction map for
pF1KE1044
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 545 CGCG CG^CG
AfaI 1123 GTAC GT^AC
AlwI 87 GATCC GGATC(4/5)
AlwNI 1178 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1330 GGGCCC GGGCC^C
Asp700I 130 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 892 GAGCTC G[AT]GC[AT]^C
AvaI 1646 CCCGGG C^[CT]CG[GA]G
BbrPI 944 CACGTG CAC^GTG
BglII 378 AGATCT A^GATCT
BlpI 1455 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1455 GCTCAGC GC^T[ACGT]AGC
BsaAI 944 CACGTG [CT]AC^GT[GA]
BsaBI 1400 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 851 GTGCAG GTGCAG(16/14)
BsiHKAI 892 GAGCTC G[AT]GC[AT]^C
BsmFI 824 GTCCC GGGAC(10/14)
BsoBI 1646 CCCGGG C^[CT]CG[GA]G
BspMI 187 ACCTGC ACCTGC(4/8)
BsrDI 362 GCAATG GCAATG(2/0)
BstUI 545 CGCG CG^CG
CelII 1455 GCTCAGC GC^T[ACGT]AGC
DraIII 1078 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 452 ATGCAT ATGCA^T
MamI 1400 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 105 CATTGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 545 CGCG CG^CG
NdeI 455 CATATG CA^TATG
NsiI 452 ATGCAT ATGCA^T
PmaCI 944 CACGTG CAC^GTG
PmlI 944 CACGTG CAC^GTG
RsaI 1123 GTAC GT^AC
SacI 892 GAGCTC GAGCT^C
SapI 974 GCTCTTC GCTCTTC(1/4)
SmaI 1646 CCCGGG CCC^GGG
SmlI 1232 CTCAAG C^T[CT][GA]AG
XbaI 12 TCTAGA T^CTAGA
XcmI 140 CCAAATCTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1646 CCCGGG C^CCGGG
XmaI 1646 CCCGGG C^CCGGG
XmnI 130 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 328 GGTGCC G^G[CT][GA]CC
1424 GGTGCC
BanII 892 GAGCTC G[GA]GC[CT]^C
1330 GGGCCC
BbsI 384 GAAGAC GAAGAC(2/6)
1287 GTCTTC
BglI 543 GCCGCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1254 GCCCAGGAGGC
BpmI 762 CTCCAG CTGGAG(16/14)
786 CTGGAG
BpuAI 384 GAAGAC GAAGAC(2/6)
1287 GTCTTC
BstXI 810 CCAGTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
983 CCAGAGGGGTGG
BstYI 86 AGATCC [GA]^GATC[CT]
378 AGATCT
DraII 550 AGGCCCC [GA]G^G[ACGT]CC[CT]
1410 AGGCCCC
EcoO109I 550 AGGCCCC [GA]G^G[ACGT]CC[CT]
1410 AGGCCCC
EcoRI 1111 GAATTC G^AATTC
1641 GAATTC
HapII 333 CCGG C^CGG
1647 CCGG
HpaII 333 CCGG C^CGG
1647 CCGG
MaeII 945 ACGT A^CGT
1529 ACGT
MflI 86 AGATCC [GA]^GATC[CT]
378 AGATCT
MspI 333 CCGG C^CGG
1647 CCGG
PstI 167 CTGCAG CTGCA^G
1658 CTGCAG
SfcI 167 CTGCAG C^T[GA][CT]AG
1658 CTGCAG
XhoII 86 AGATCC [GA]^GATC[CT]
378 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BcnI BfaI BmyI BsaI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstEII BstF5I
BstNI BstPI Cac8I Cfr13I DdeI
DpnII EarI EcoO65I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I