Restriction map for
pF1KB0983
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI HphI
KasI KspI MaeI MaeII MaeIII
MamI MflI MluI MluNI MroI
MscI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I XbaI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 282 GGTACC G^GTACC
AcsI 114 GAATTT [GA]^AATT[CT]
AcyI 630 GACGCC G[GA]^CG[CT]C
AlwI 120 GATCC GGATC(4/5)
ApaI 597 GGGCCC GGGCC^C
ApoI 114 GAATTT [GA]^AATT[CT]
Asp718I 282 GGTACC G^GTACC
BcnI 652 CCGGG CC^[GC]GG
BsaHI 630 GACGCC G[GA]^CG[CT]C
BspMI 417 GCAGGT ACCTGC(4/8)
BsrDI 273 CATTGC GCAATG(2/0)
BstAPI 451 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 410 CATCC GGATG(2/0)
BstXI 236 CCATGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 593 CCATGG C^C[GA][CT]GG
EaeI 69 TGGCCG [CT]^GGCC[GA]
EarI 437 GAAGAG CTCTTC(1/4)
EcoT14I 593 CCATGG C^C[AT][AT]GG
FauI 192 GCGGG CCCGC(4/6)
FokI 410 CATCC GGATG(9/13)
HapII 652 CCGG C^CGG
Hin1I 630 GACGCC G[GA]^CG[CT]C
HpaII 652 CCGG C^CGG
KpnI 282 GGTACC GGTAC^C
Ksp632I 437 GAAGAG CTCTTC(1/4)
MfeI 605 CAATTG C^AATTG
MseI 92 TTAA T^TAA
MslI 237 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 652 CCGG C^CGG
MunI 605 CAATTG C^AATTG
NciI 652 CCGGG CC^[GC]GG
NcoI 593 CCATGG C^CATGG
NdeI 109 CATATG CA^TATG
NspI 641 GCATGC [GA]CATG^[CT]
PaeR7I 623 CTCGAG C^TCGAG
PleI 426 GACTC GAGTC(4/5)
PstI 28 CTGCAG CTGCA^G
SexAI 576 ACCTGGT A^CC[AT]GGT
SfaNI 24 GATGC GCATC(5/9)
SmlI 623 CTCGAG C^T[CT][GA]AG
SphI 641 GCATGC GCATG^C
StyI 593 CCATGG C^C[AT][AT]GG
TaqI 624 TCGA T^CGA
TfiI 74 GAATC G^A[AT]TC
Tru9I 92 TTAA T^TAA
TspRI 444 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 624 TCGA T^CGA
XcmI 363 CCAGGCCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 623 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 383 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
452 CAGCTGCTG
AvaI 278 CTCGGG C^[CT]CG[GA]G
623 CTCGAG
AvaII 406 GGACC G^G[AT]CC
685 GGTCC
BanI 85 GGCACC G^G[CT][GA]CC
282 GGTACC
BanII 488 GAGCCC G[GA]GC[CT]^C
597 GGGCCC
BciVI 56 GTATCC GTATCC(6/5)
232 GTATCC
BsaI 585 GAGACC GGTCTC(1/5)
660 GAGACC
BsmAI 585 GAGAC GTCTC(1/5)
660 GAGAC
BsmFI 405 GGGAC GGGAC(10/14)
628 GGGAC
BsmI 162 GAATGC GAATGC(1/-1)
701 GAATGC
BsoBI 278 CTCGGG C^[CT]CG[GA]G
623 CTCGAG
BsrI 268 ACTGG ACTGG(1/-1)
476 CCAGT
Cac8I 382 GCAGGC GC[ACGT]^[ACGT]GC
641 GCATGC
DpnII 120 GATC ^GATC
150 GATC
HgaI 630 GACGC GACGC(5/10)
705 GCGTC
HinfI 74 GAATC G^A[ACGT]TC
426 GACTC
MboI 120 GATC ^GATC
150 GATC
NdeII 120 GATC ^GATC
150 GATC
PflMI 286 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
601 CCACCAATTGG
Sau3AI 120 GATC ^GATC
150 GATC
Van91I 286 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
601 CCACCAATTGG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI BbvI BmyI
BpmI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstNI Cfr13I DraII
EcoNI EcoO109I EcoRII Fnu4HI HaeIII
ItaI MboII MnlI MspA1I MvaI
MwoI NlaIII NlaIV PvuII RsaI
Sau96I ScrFI SfcI TseI Tsp509I