Restriction map for
pF1KE1016
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaI BsiEI BsiWI
Bsp1407I BspHI BspLU11I BspMI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 1044 GTCTAC GT^[AC][GT]AC
AccIII 554 TCCGGA T^CCGGA
AclI 715 AACGTT AA^CGTT
AcsI 702 GAATTT [GA]^AATT[CT]
AfaI 1146 GTAC GT^AC
Alw44I 364 GTGCAC G^TGCAC
ApaLI 364 GTGCAC G^TGCAC
ApoI 702 GAATTT [GA]^AATT[CT]
AvaI 85 CCCGAG C^[CT]CG[GA]G
BamHI 690 GGATCC G^GATCC
BanII 618 GAGCCC G[GA]GC[CT]^C
BbsI 1023 GTCTTC GAAGAC(2/6)
BglI 746 GCCCTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 141 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 141 GCTGAGC GC^T[ACGT]AGC
BpuAI 1023 GTCTTC GAAGAC(2/6)
BsaAI 265 TACGTG [CT]AC^GT[GA]
BsaWI 554 TCCGGA [AT]^CCGG[AT]
BseAI 554 TCCGGA T^CCGGA
BsmBI 810 CGTCTC CGTCTC(1/5)
BsmI 499 GCATTC GAATGC(1/-1)
BsoBI 85 CCCGAG C^[CT]CG[GA]G
BspDI 769 ATCGAT AT^CGAT
BspEI 554 TCCGGA T^CCGGA
BsrDI 392 GCAATG GCAATG(2/0)
BsrFI 314 GCCGGC [GA]^CCGG[CT]
BssHII 49 GCGCGC G^CGCGC
BssSI 632 CTCGTG CACGAG(-5/-1)
BstXI 607 CCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 690 GGATCC [GA]^GATC[CT]
CelII 141 GCTGAGC GC^T[ACGT]AGC
Cfr10I 314 GCCGGC [GA]^CCGG[CT]
ClaI 769 ATCGAT AT^CGAT
DraIII 332 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1014 GACCCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 840 GAAGAG CTCTTC(1/4)
EcoRV 766 GATATC GAT^ATC
Ksp632I 840 GAAGAG CTCTTC(1/4)
KspI 1174 CCGCGG CCGC^GG
MaeIII 236 GTGAC ^GT[ACGT]AC
MflI 690 GGATCC [GA]^GATC[CT]
MroI 554 TCCGGA T^CCGGA
MseI 296 TTAA T^TAA
MslI 367 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 314 GCCGGC GCC^GGC
NcoI 1208 CCATGG C^CATGG
NgoMIV 314 GCCGGC G^CCGGC
PshAI 804 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 715 AACGTT AA^CGTT
RsaI 1146 GTAC GT^AC
SacII 1174 CCGCGG CCGC^GG
SapI 840 GAAGAGC GCTCTTC(1/4)
StuI 20 AGGCCT AGG^CCT
TaqI 770 TCGA T^CGA
Tru9I 296 TTAA T^TAA
Tsp45I 236 GTGAC ^GT[GC]AC
TspRI 234 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 770 TCGA T^CGA
XhoII 690 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 91 CCTGAGG CC^T[ACGT]AGG
964 CCTGAGG
BalI 254 TGGCCA TGG^CCA
583 TGGCCA
BsgI 423 GTGCAG GTGCAG(16/14)
635 GTGCAG
BsmAI 811 GTCTC GTCTC(1/5)
915 GTCTC
BsrBI 133 GAGCGG CCGCTC(-3/-3)
1274 GAGCGG
BstAPI 425 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
860 GCAGCTGCTGC
Bsu36I 91 CCTGAGG CC^T[ACGT]AGG
964 CCTGAGG
DraII 123 GGGACCC [GA]G^G[ACGT]CC[CT]
1116 GGGACCC
Eco81I 91 CCTGAGG CC^T[ACGT]AGG
964 CCTGAGG
EcoO109I 123 GGGACCC [GA]G^G[ACGT]CC[CT]
1116 GGGACCC
EcoT14I 944 CCTTGG C^C[AT][AT]GG
1208 CCATGG
MluNI 254 TGGCCA TGG^CCA
583 TGGCCA
MscI 254 TGGCCA TGG^CCA
583 TGGCCA
PpuMI 123 GGGACCC [GA]G^G[AT]CC[CT]
1116 GGGACCC
PstI 78 CTGCAG CTGCA^G
1249 CTGCAG
PvuII 734 CAGCTG CAG^CTG
861 CAGCTG
StyI 944 CCTTGG C^C[AT][AT]GG
1208 CCATGG
Tsp509I 245 AATT ^AATT
703 AATT
XcmI 604 CCACCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
908 CCACGCAGTCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaII BanI BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoNI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI TfiI TseI