Restriction map for
pF1KE2341
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AvaI
AviII AvrII BbeI BbrPI BcgI
BclI BcnI BfrI BglI BlnI
BsaAI BsaBI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HaeII
HpaI KasI KpnI Ksp632I KspI
MamI MluI MroI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SmaI SmlI SnaBI
SphI Sse8387I StuI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 951 GTCTAC GT^[AC][GT]AC
AccII 48 CGCG CG^CG
AcyI 1895 GACGCC G[GA]^CG[CT]C
Asp700I 2118 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 654 GGCACC G^G[CT][GA]CC
BanII 3 GGGCTC G[GA]GC[CT]^C
BbvCI 12 GCTGAGG CCTCAGC(-5/-2)
BciVI 860 GTATCC GTATCC(6/5)
BlpI 2163 GCTGAGC GC^T[ACGT]AGC
BmyI 3 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 2163 GCTGAGC GC^T[ACGT]AGC
BsaHI 1895 GACGCC G[GA]^CG[CT]C
BsaWI 1309 TCCGGT [AT]^CCGG[AT]
BsmFI 128 GGGAC GGGAC(10/14)
Bsp1286I 3 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 1603 TGTACA T^GTACA
BspMI 1570 GCAGGT ACCTGC(4/8)
BsrBI 748 GAGCGG CCGCTC(-3/-3)
BsrDI 573 CATTGC GCAATG(2/0)
BsrFI 43 ACCGGC [GA]^CCGG[CT]
BsrGI 1603 TGTACA T^GTACA
BssSI 679 CACGAG CACGAG(-5/-1)
BstEII 39 GGTCACC G^GT[ACGT]ACC
BstPI 39 GGTCACC G^GT[ACGT]ACC
BstUI 48 CGCG CG^CG
BstXI 1770 CCAAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2163 GCTGAGC GC^T[ACGT]AGC
CfoI 47 GCGC GCG^C
Cfr10I 43 ACCGGC [GA]^CCGG[CT]
DraII 2031 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 2031 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 39 GGTCACC G^GT[ACGT]ACC
EcoT22I 1799 ATGCAT ATGCA^T
HhaI 47 GCGC GCG^C
Hin1I 1895 GACGCC G[GA]^CG[CT]C
HinP1I 47 GCGC G^CGC
HincII 289 GTCAAC GT[CT]^[GA]AC
HindII 289 GTCAAC GT[CT]^[GA]AC
MaeII 949 ACGT A^CGT
MfeI 536 CAATTG C^AATTG
MslI 2085 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 10 CCGCTG C[AC]G^C[GT]G
MunI 536 CAATTG C^AATTG
MvnI 48 CGCG CG^CG
NdeI 1802 CATATG CA^TATG
NsiI 1799 ATGCAT ATGCA^T
PflMI 1819 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 1226 AGTACT AGT^ACT
SgrAI 42 CACCGGCG C[GA]^CCGG[CT]G
SpeI 937 ACTAGT A^CTAGT
SspBI 1603 TGTACA T^GTACA
SspI 1975 AATATT AAT^ATT
TaqI 20 TCGA T^CGA
TthHB8I 20 TCGA T^CGA
Van91I 1819 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 864 CCAAGAATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2118 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 796 CCTGAGG CC^T[ACGT]AGG
1918 CCTGAGG
AspI 1426 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1783 GACATTGTC
AvaII 1767 GGTCC G^G[AT]CC
2094 GGTCC
BamHI 1068 GGATCC G^GATCC
1306 GGATCC
BglII 621 AGATCT A^GATCT
824 AGATCT
BsmAI 1526 GAGAC GTCTC(1/5)
1781 GAGAC
BsmI 840 GAATGC GAATGC(1/-1)
2122 GCATTC
BstAPI 1279 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2038 GCAAAAGGTGC
Bsu36I 796 CCTGAGG CC^T[ACGT]AGG
1918 CCTGAGG
Eco81I 796 CCTGAGG CC^T[ACGT]AGG
1918 CCTGAGG
EcoNI 320 CCTTTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2035 CCTGCAAAAGG
EcoRI 302 GAATTC G^AATTC
1748 GAATTC
HapII 44 CCGG C^CGG
1310 CCGG
HgaI 1895 GACGC GACGC(5/10)
1057 GCGTC
HindIII 1861 AAGCTT A^AGCTT
2104 AAGCTT
HpaII 44 CCGG C^CGG
1310 CCGG
MspI 44 CCGG C^CGG
1310 CCGG
PflFI 1426 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1783 GACATTGTC
SfaNI 262 GATGC GCATC(5/9)
1468 GATGC
SfcI 953 CTACAG C^T[GA][CT]AG
1442 CTGTAG
Tth111I 1426 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1783 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BalI BbsI BbvI BfaI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII