Restriction map for
pF1KE2729
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AvaI AviII BalI BamHI
BbrPI BcgI BciVI BclI BfrI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 531 GGCGCC G[GA]^CG[CT]C
AlwI 577 GATCC GGATC(4/5)
AocI 793 CCTGAGG CC^T[ACGT]AGG
Aor51HI 839 AGCGCT AGC^GCT
ApaI 1343 GGGCCC GGGCC^C
Asp700I 49 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 493 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 513 CCTAGG C^CTAGG
BbeI 531 GGCGCC GGCGC^C
BglI 534 GCCATCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 513 CCTAGG C^CTAGG
BsaHI 531 GGCGCC G[GA]^CG[CT]C
BsgI 1120 GTGCAG GTGCAG(16/14)
Bsp1407I 140 TGTACA T^GTACA
BsrDI 618 CATTGC GCAATG(2/0)
BsrGI 140 TGTACA T^GTACA
BstAPI 950 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 793 CCTGAGG CC^T[ACGT]AGG
DraII 1343 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 839 AGCGCT AGC^GCT
Eco81I 793 CCTGAGG CC^T[ACGT]AGG
EcoNI 1347 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1343 GGGCCCT [GA]G^G[ACGT]CC[CT]
Hin1I 531 GGCGCC G[GA]^CG[CT]C
HindIII 1303 AAGCTT A^AGCTT
KasI 531 GGCGCC G^GCGCC
MaeII 458 ACGT A^CGT
MseI 1223 TTAA T^TAA
MslI 421 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1205 CAGCTG C[AC]G^C[GT]G
NarI 531 GGCGCC GG^CGCC
NcoI 1114 CCATGG C^CATGG
PflFI 493 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1205 CAGCTG CAG^CTG
SapI 1197 GCTCTTC GCTCTTC(1/4)
SfoI 531 GGCGCC GGC^GCC
SspBI 140 TGTACA T^GTACA
TfiI 658 GAATC G^A[AT]TC
Tru9I 1223 TTAA T^TAA
Tth111I 493 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1353 CCAGGCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 49 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 141 GTAC GT^AC
734 GTAC
BanI 193 GGTGCC G^G[CT][GA]CC
531 GGCGCC
BbsI 1369 GAAGAC GAAGAC(2/6)
808 GTCTTC
BbvCI 587 CCTCAGC CCTCAGC(-5/-2)
1207 GCTGAGG
BpuAI 1369 GAAGAC GAAGAC(2/6)
808 GTCTTC
BseRI 78 GAGGAG GAGGAG(10/8)
1264 GAGGAG
BsmBI 286 GAGACG CGTCTC(1/5)
1338 GAGACG
DpnII 577 GATC ^GATC
631 GATC
DsaI 1114 CCATGG C^C[GA][CT]GG
1362 CCGTGG
EarI 1198 CTCTTC CTCTTC(1/4)
1246 GAAGAG
EcoT14I 513 CCTAGG C^C[AT][AT]GG
1114 CCATGG
HgaI 288 GACGC GACGC(5/10)
1194 GACGC
Ksp632I 1198 CTCTTC CTCTTC(1/4)
1246 GAAGAG
MboI 577 GATC ^GATC
631 GATC
NdeII 577 GATC ^GATC
631 GATC
PleI 112 GACTC GAGTC(4/5)
1145 GACTC
RsaI 141 GTAC GT^AC
734 GTAC
Sau3AI 577 GATC ^GATC
631 GATC
SfaNI 1072 GATGC GCATC(5/9)
980 GCATC
StyI 513 CCTAGG C^C[AT][AT]GG
1114 CCATGG
TaqI 239 TCGA T^CGA
276 TCGA
Tsp509I 158 AATT ^AATT
377 AATT
TthHB8I 239 TCGA T^CGA
276 TCGA
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboII
MnlI MspI MvaI MwoI NciI
NlaIII NlaIV PstI Sau96I ScrFI
SfcI TseI Tsp45I TspRI