Restriction map for
pF1KB6686
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbsI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 921 GTCTAC GT^[AC][GT]AC
AcsI 745 GAATTT [GA]^AATT[CT]
AcyI 1237 GACGCC G[GA]^CG[CT]C
AflIII 1186 ACACGT A^C[GA][CT]GT
ApoI 745 GAATTT [GA]^AATT[CT]
AvaI 1225 CTCGAG C^[CT]CG[GA]G
BanI 416 GGCACC G^G[CT][GA]CC
BcgI 1217 GCAGCCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 547 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 678 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1237 GACGCC G[GA]^CG[CT]C
BsgI 899 GTGCAG GTGCAG(16/14)
BsiEI 336 CGACCG CG[GA][CT]^CG
BsmBI 1051 GAGACG CGTCTC(1/5)
BsoBI 1225 CTCGAG C^[CT]CG[GA]G
BsrBI 445 GAGCGG CCGCTC(-3/-3)
BsrDI 305 GCAATG GCAATG(2/0)
BssHII 366 GCGCGC G^CGCGC
EaeI 765 CGGCCA [CT]^GGCC[GA]
Hin1I 1237 GACGCC G[GA]^CG[CT]C
MamI 678 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 930 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 848 CCATGG C^CATGG
PaeR7I 1225 CTCGAG C^TCGAG
PleI 1094 GACTC GAGTC(4/5)
SexAI 755 ACCAGGT A^CC[AT]GGT
TfiI 939 GATTC G^A[AT]TC
Tsp45I 410 GTGAC ^GT[GC]AC
Tsp509I 746 AATT ^AATT
XhoI 1225 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 367 CGCG CG^CG
814 CGCG
AfaI 840 GTAC GT^AC
1161 GTAC
BbvCI 1262 CCTCAGC CCTCAGC(-5/-2)
874 GCTGAGG
BlpI 1014 GCTGAGC GC^T[ACGT]AGC
1119 GCTGAGC
Bpu1102I 1014 GCTGAGC GC^T[ACGT]AGC
1119 GCTGAGC
BsmAI 1051 GAGAC GTCTC(1/5)
85 GTCTC
BsrI 301 ACTGG ACTGG(1/-1)
413 ACTGG
BstUI 367 CGCG CG^CG
814 CGCG
CelII 1014 GCTGAGC GC^T[ACGT]AGC
1119 GCTGAGC
DsaI 78 CCGTGG C^C[GA][CT]GG
848 CCATGG
HgaI 1237 GACGC GACGC(5/10)
1274 GCGTC
HincII 268 GTTGAC GT[CT]^[GA]AC
772 GTCAAC
HindII 268 GTTGAC GT[CT]^[GA]AC
772 GTCAAC
HinfI 939 GATTC G^A[ACGT]TC
1094 GACTC
HphI 399 GGTGA GGTGA(8/7)
317 TCACC
MaeII 776 ACGT A^CGT
1188 ACGT
MvnI 367 CGCG CG^CG
814 CGCG
NheI 436 GCTAGC G^CTAGC
705 GCTAGC
PflMI 603 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
984 CCAAGGCCTGG
RsaI 840 GTAC GT^AC
1161 GTAC
SapI 478 GAAGAGC GCTCTTC(1/4)
700 GAAGAGC
SmlI 574 CTCAAG C^T[CT][GA]AG
1225 CTCGAG
StuI 987 AGGCCT AGG^CCT
1369 AGGCCT
Van91I 603 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
984 CCAAGGCCTGG
XcmI 383 CCAACGCTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
548 CCAGGCTGGCTGTGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwI AspHI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoNI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
TspRI TthHB8I XhoII