Restriction map for
pF1KB6520
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# NO CUT ENZYMES ( 120 enzymes) #
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AccI AclI AcsI AflII AflIII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I AspI AviII
AvrII BalI BbrPI BbvCI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsgI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MaeI MamI MfeI MluI MluNI
MscI MseI MunI NaeI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StyI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 886 GACGTC GACGT^C
AccIII 301 TCCGGA T^CCGGA
Alw44I 400 GTGCAC G^TGCAC
AlwNI 996 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 832 GGGCCC GGGCC^C
ApaLI 400 GTGCAC G^TGCAC
BbeI 727 GGCGCC GGCGC^C
BcgI 343 CGATGCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 371 GCCTGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 105 AGATCT A^GATCT
BsaWI 301 TCCGGA [AT]^CCGG[AT]
BseAI 301 TCCGGA T^CCGGA
BsiEI 504 CGACCG CG[GA][CT]^CG
BsmBI 307 GAGACG CGTCTC(1/5)
BsmI 954 GAATGC GAATGC(1/-1)
BspEI 301 TCCGGA T^CCGGA
BsrFI 791 ACCGGC [GA]^CCGG[CT]
BstBI 535 TTCGAA TT^CGAA
BstXI 1079 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 791 ACCGGC [GA]^CCGG[CT]
DdeI 1111 CTAAG C^T[ACGT]AG
DrdI 880 GACCTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 936 CCACGG C^C[GA][CT]GG
EarI 250 CTCTTC CTCTTC(1/4)
FauI 452 GCGGG CCCGC(4/6)
HaeII 727 GGCGCC [GA]GCGC^[CT]
KasI 727 GGCGCC G^GCGCC
Ksp632I 250 CTCTTC CTCTTC(1/4)
MroI 301 TCCGGA T^CCGGA
MspA1I 768 CAGCTG C[AC]G^C[GT]G
NarI 727 GGCGCC GG^CGCC
NdeI 116 CATATG CA^TATG
NspI 657 GCATGT [GA]CATG^[CT]
NspV 535 TTCGAA TT^CGAA
PleI 511 GAGTC GAGTC(4/5)
PvuII 768 CAGCTG CAG^CTG
SfoI 727 GGCGCC GGC^GCC
SfuI 535 TTCGAA TT^CGAA
SspI 1118 AATATT AAT^ATT
StuI 494 AGGCCT AGG^CCT
TfiI 519 GAATC G^A[AT]TC
Tsp509I 1025 AATT ^AATT
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# TWO CUT ENZYMES ( 26 enzymes) #
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Acc65I 272 GGTACC G^GTACC
773 GGTACC
AccII 964 CGCG CG^CG
1065 CGCG
AcyI 727 GGCGCC G[GA]^CG[CT]C
886 GACGTC
AhdI 505 GACCGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
560 GACAGCCAGTC
Asp718I 272 GGTACC G^GTACC
773 GGTACC
AspEI 505 GACCGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
560 GACAGCCAGTC
BamHI 61 GGATCC G^GATCC
943 GGATCC
BpmI 175 CTCCAG CTGGAG(16/14)
904 CTGGAG
BsaHI 727 GGCGCC G[GA]^CG[CT]C
886 GACGTC
BseRI 555 GAGGAG GAGGAG(10/8)
697 GAGGAG
BstAPI 158 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
338 GCACCCGATGC
BstUI 964 CGCG CG^CG
1065 CGCG
EaeI 5 CGGCCA [CT]^GGCC[GA]
1067 CGGCCA
Eam1105I 505 GACCGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
560 GACAGCCAGTC
HgaI 309 GACGC GACGC(5/10)
465 GCGTC
Hin1I 727 GGCGCC G[GA]^CG[CT]C
886 GACGTC
HinfI 511 GAGTC G^A[ACGT]TC
519 GAATC
KpnI 272 GGTACC GGTAC^C
773 GGTACC
MaeII 47 ACGT A^CGT
887 ACGT
MslI 42 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
748 CACACCTGTG
MvnI 964 CGCG CG^CG
1065 CGCG
PpuMI 385 GGGACCT [GA]G^G[AT]CC[CT]
705 GGGACCC
PstI 460 CTGCAG CTGCA^G
799 CTGCAG
SacI 70 GAGCTC GAGCT^C
1053 GAGCTC
SfcI 460 CTGCAG C^T[GA][CT]AG
799 CTGCAG
XcmI 8 CCATCCCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
731 CCACACCTCCCCTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI SmlI TaqI TseI Tsp45I
TspRI TthHB8I XhoII XmaCI XmaI