Restriction map for
pF1KE0702
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# NO CUT ENZYMES ( 118 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BciVI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI ScaI
SfoI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 82 GACGTC GACGT^C
AccI 640 GTCGAC GT^[AC][GT]AC
AcyI 82 GACGTC G[GA]^CG[CT]C
AlwNI 712 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1155 GGGCCC GGGCC^C
AspHI 102 GAGCTC G[AT]GC[AT]^C
BalI 138 TGGCCA TGG^CCA
BamHI 822 GGATCC G^GATCC
BbsI 294 GTCTTC GAAGAC(2/6)
BclI 731 TGATCA T^GATCA
BglI 1131 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 456 AGATCT A^GATCT
BpuAI 294 GTCTTC GAAGAC(2/6)
BsaHI 82 GACGTC G[GA]^CG[CT]C
BsaI 404 GGTCTC GGTCTC(1/5)
BsiHKAI 102 GAGCTC G[AT]GC[AT]^C
BsmI 999 GAATGC GAATGC(1/-1)
BspDI 280 ATCGAT AT^CGAT
BspMI 816 GCAGGT ACCTGC(4/8)
BsrBI 178 GAGCGG CCGCTC(-3/-3)
BssSI 142 CACGAG CACGAG(-5/-1)
BstXI 691 CCACCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 280 ATCGAT AT^CGAT
DsaI 605 CCATGG C^C[GA][CT]GG
EarI 1162 GAAGAG CTCTTC(1/4)
EcoNI 240 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 605 CCATGG C^C[AT][AT]GG
FbaI 731 TGATCA T^GATCA
HaeII 582 AGCGCC [GA]GCGC^[CT]
HgaI 3 GACGC GACGC(5/10)
Hin1I 82 GACGTC G[GA]^CG[CT]C
HincII 640 GTCGAC GT[CT]^[GA]AC
HindII 640 GTCGAC GT[CT]^[GA]AC
HindIII 547 AAGCTT A^AGCTT
Ksp632I 1162 GAAGAG CTCTTC(1/4)
MluNI 138 TGGCCA TGG^CCA
MscI 138 TGGCCA TGG^CCA
MseI 596 TTAA T^TAA
MslI 729 CATGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 253 GCCGGC GCC^GGC
NcoI 605 CCATGG C^CATGG
NgoMIV 253 GCCGGC G^CCGGC
PflMI 622 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 814 CTGCAG CTGCA^G
SacI 102 GAGCTC GAGCT^C
SalI 640 GTCGAC G^TCGAC
SexAI 388 ACCAGGT A^CC[AT]GGT
SfiI 1130 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 703 CACCGGCG C[GA]^CCGG[CT]G
SspI 472 AATATT AAT^ATT
StyI 605 CCATGG C^C[AT][AT]GG
TfiI 283 GATTC G^A[AT]TC
Tru9I 596 TTAA T^TAA
Tsp45I 169 GTGAC ^GT[GC]AC
Van91I 622 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AocI 76 CCTAAGG CC^T[ACGT]AGG
826 CCTGAGG
AvaI 466 CCCGAG C^[CT]CG[GA]G
763 CCCGAG
BanI 701 GGCACC G^G[CT][GA]CC
1040 GGCACC
BanII 102 GAGCTC G[GA]GC[CT]^C
1155 GGGCCC
BcgI 1050 CGACAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
632 GCAAGGAGGTCG
BcnI 924 CCGGG CC^[GC]GG
1107 CCGGG
BlpI 175 GCTGAGC GC^T[ACGT]AGC
844 GCTGAGC
BpmI 804 CTCCAG CTGGAG(16/14)
199 CTGGAG
Bpu1102I 175 GCTGAGC GC^T[ACGT]AGC
844 GCTGAGC
BsmFI 565 GGGAC GGGAC(10/14)
1013 GGGAC
BsoBI 466 CCCGAG C^[CT]CG[GA]G
763 CCCGAG
Bsu36I 76 CCTAAGG CC^T[ACGT]AGG
826 CCTGAGG
CelII 175 GCTGAGC GC^T[ACGT]AGC
844 GCTGAGC
CfoI 583 GCGC GCG^C
1024 GCGC
Eco81I 76 CCTAAGG CC^T[ACGT]AGG
826 CCTGAGG
FauI 911 CCCGC CCCGC(4/6)
210 GCGGG
HhaI 583 GCGC GCG^C
1024 GCGC
HinP1I 583 GCGC G^CGC
1024 GCGC
MaeII 11 ACGT A^CGT
83 ACGT
MaeIII 169 GTGAC ^GT[ACGT]AC
723 GTTAC
NciI 924 CCGGG CC^[GC]GG
1107 CCGGG
PpuMI 565 GGGACCC [GA]G^G[AT]CC[CT]
1013 GGGACCC
SfaNI 327 GATGC GCATC(5/9)
450 GCATC
SfcI 814 CTGCAG C^T[GA][CT]AG
856 CTACAG
XcmI 528 CCACTTGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
618 CCACCCAGGCTATGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
TaqI TseI TspRI TthHB8I XhoII