Restriction map for
pF1KE1098
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SwaI TspRI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AlwI 74 GATCC GGATC(4/5)
AocI 40 CCTGAGG CC^T[ACGT]AGG
Asp700I 263 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 442 GGTGCC G^G[CT][GA]CC
BanII 820 GAGCCC G[GA]GC[CT]^C
BbsI 295 GAAGAC GAAGAC(2/6)
BbvCI 346 GCTGAGG CCTCAGC(-5/-2)
BbvI 375 GCAGC GCAGC(8/12)
BciVI 164 GTATCC GTATCC(6/5)
BcnI 4 CCGGG CC^[GC]GG
BmyI 820 GAGCCC G[AGT]GC[ACT]^C
BpuAI 295 GAAGAC GAAGAC(2/6)
BsaI 429 GAGACC GGTCTC(1/5)
BsiYI 433 CCTGGATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 433 CCTGGATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 329 GCATTC GAATGC(1/-1)
Bsp1286I 820 GAGCCC G[AGT]GC[ACT]^C
BspMI 640 GCAGGT ACCTGC(4/8)
BsrDI 78 CATTGC GCAATG(2/0)
BstYI 73 AGATCC [GA]^GATC[CT]
Bsu36I 40 CCTGAGG CC^T[ACGT]AGG
CfoI 546 GCGC GCG^C
DpnII 74 GATC ^GATC
EarI 263 GAAGAG CTCTTC(1/4)
Eco81I 40 CCTGAGG CC^T[ACGT]AGG
EcoT14I 365 CCAAGG C^C[AT][AT]GG
EcoT22I 452 ATGCAT ATGCA^T
Fnu4HI 375 GCAGC GC^[ACGT]GC
HaeII 545 GGCGCT [GA]GCGC^[CT]
HaeIII 739 GGCC GG^CC
HapII 4 CCGG C^CGG
HhaI 546 GCGC GCG^C
HinP1I 546 GCGC G^CGC
HinfI 135 GATTC G^A[ACGT]TC
HpaII 4 CCGG C^CGG
HphI 63 GGTGA GGTGA(8/7)
ItaI 375 GCAGC GC^[ACGT]GC
Ksp632I 263 GAAGAG CTCTTC(1/4)
MaeIII 832 GTCAC ^GT[ACGT]AC
MboI 74 GATC ^GATC
MflI 73 AGATCC [GA]^GATC[CT]
MslI 219 CATTAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 376 CAGCTG C[AC]G^C[GT]G
MspI 4 CCGG C^CGG
NciI 4 CCGGG CC^[GC]GG
NdeII 74 GATC ^GATC
NsiI 452 ATGCAT ATGCA^T
PvuII 376 CAGCTG CAG^CTG
Sau3AI 74 GATC ^GATC
ScaI 522 AGTACT AGT^ACT
SfcI 656 CTACAG C^T[GA][CT]AG
SphI 462 GCATGC GCATG^C
SspI 535 AATATT AAT^ATT
StuI 738 AGGCCT AGG^CCT
StyI 365 CCAAGG C^C[AT][AT]GG
TaqI 573 TCGA T^CGA
TfiI 135 GATTC G^A[AT]TC
TseI 375 GCAGC G^C[AT]GC
Tsp45I 832 GTCAC ^GT[GC]AC
TthHB8I 573 TCGA T^CGA
XcmI 77 CCATTGCTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 73 AGATCC [GA]^GATC[CT]
XmnI 263 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 8 enzymes) #
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AfaI 423 GTAC GT^AC
523 GTAC
BpmI 272 CTCCAG CTGGAG(16/14)
724 CTGGAG
BseRI 692 CTCCTC GAGGAG(10/8)
695 CTCCTC
Cac8I 104 GCTTGC GC[ACGT]^[ACGT]GC
462 GCATGC
MwoI 445 GCCTATGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
631 GCCCTAAGAGC
NlaIV 442 GGTGCC GG[ACGT]^[ACGT]CC
819 GGAGCC
NspI 462 GCATGC [GA]CATG^[CT]
665 ACATGC
RsaI 423 GTAC GT^AC
523 GTAC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AcsI AluI ApoI AseI AsnI
BsaJI BsmAI BssKI BstF5I BstNI
DdeI EcoRII FokI MboII MnlI
MseI MvaI NlaIII PshBI ScrFI
SfaNI Tru9I Tsp509I