Restriction map for
pF1KB6176
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AclI AcsI AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BlnI
BsaAI BsaBI BsaI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KspI MamI MluI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1471 GACGTC GACGT^C
AccIII 484 TCCGGA T^CCGGA
AflII 346 CTTAAG C^TTAAG
AflIII 1086 ACATGT A^C[GA][CT]GT
AocI 1397 CCTCAGG CC^T[ACGT]AGG
Asp700I 309 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1204 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1628 GGCGCC GGCGC^C
BfaI 133 CTAG C^TAG
BfrI 346 CTTAAG C^TTAAG
BglII 1284 AGATCT A^GATCT
BlpI 1033 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1033 GCTGAGC GC^T[ACGT]AGC
BsaWI 484 TCCGGA [AT]^CCGG[AT]
BseAI 484 TCCGGA T^CCGGA
BsgI 928 GTGCAG GTGCAG(16/14)
BsiEI 1123 CGGCCG CG[GA][CT]^CG
BspEI 484 TCCGGA T^CCGGA
BspHI 1115 TCATGA T^CATGA
BspLU11I 1086 ACATGT A^CATGT
BsrDI 231 CATTGC GCAATG(2/0)
BsrFI 730 GCCGGT [GA]^CCGG[CT]
Bst1107I 67 GTATAC GTA^TAC
BstZ17I 67 GTATAC GTA^TAC
Bsu36I 1397 CCTCAGG CC^T[ACGT]AGG
CelII 1033 GCTGAGC GC^T[ACGT]AGC
Cfr10I 730 GCCGGT [GA]^CCGG[CT]
EagI 1123 CGGCCG C^GGCCG
EclXI 1123 CGGCCG C^GGCCG
Eco52I 1123 CGGCCG C^GGCCG
Eco81I 1397 CCTCAGG CC^T[ACGT]AGG
HaeII 1628 GGCGCC [GA]GCGC^[CT]
HgaI 1080 GACGC GACGC(5/10)
KasI 1628 GGCGCC G^GCGCC
MaeI 133 CTAG C^TAG
MfeI 1697 CAATTG C^AATTG
MroI 484 TCCGGA T^CCGGA
MseI 347 TTAA T^TAA
MunI 1697 CAATTG C^AATTG
NarI 1628 GGCGCC GG^CGCC
NdeI 655 CATATG CA^TATG
PflFI 1204 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1115 TCATGA T^CATGA
SacI 253 GAGCTC GAGCT^C
SfoI 1628 GGCGCC GGC^GCC
Tru9I 347 TTAA T^TAA
Tth111I 1204 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 132 TCTAGA T^CTAGA
XmnI 309 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 884 GGTACC G^GTACC
912 GGTACC
AccII 835 CGCG CG^CG
1082 CGCG
AcyI 1471 GACGTC G[GA]^CG[CT]C
1628 GGCGCC
ApaI 15 GGGCCC GGGCC^C
1376 GGGCCC
Asp718I 884 GGTACC G^GTACC
912 GGTACC
BbvCI 1590 CCTCAGC CCTCAGC(-5/-2)
1423 GCTGAGG
BglI 25 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1529 GCCACATTGGC
BsaHI 1471 GACGTC G[GA]^CG[CT]C
1628 GGCGCC
BstUI 835 CGCG CG^CG
1082 CGCG
BstXI 375 CCAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
696 CCAGCTCACTGG
DraIII 917 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1143 CACACAGTG
EarI 1675 CTCTTC CTCTTC(1/4)
939 GAAGAG
EcoNI 791 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1308 CCTACATGAGG
Hin1I 1471 GACGTC G[GA]^CG[CT]C
1628 GGCGCC
HincII 433 GTTGAC GT[CT]^[GA]AC
1090 GTCAAC
HindII 433 GTTGAC GT[CT]^[GA]AC
1090 GTCAAC
KpnI 884 GGTACC GGTAC^C
912 GGTACC
Ksp632I 1675 CTCTTC CTCTTC(1/4)
939 GAAGAG
MvnI 835 CGCG CG^CG
1082 CGCG
NcoI 360 CCATGG C^CATGG
677 CCATGG
NspI 1086 ACATGT [GA]CATG^[CT]
1182 GCATGT
SfcI 619 CTACAG C^T[GA][CT]AG
1455 CTACAG
SmaI 44 CCCGGG CCC^GGG
761 CCCGGG
Tsp509I 298 AATT ^AATT
1698 AATT
XmaCI 44 CCCGGG C^CCGGG
761 CCCGGG
XmaI 44 CCCGGG C^CCGGG
761 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII