Restriction map for
pF1KB6441
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspHI AspI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FseI FspI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MamI MluI MluNI MroI
MscI MspA1I MspI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 799 AAATTC [GA]^AATT[CT]
AfaI 739 GTAC GT^AC
AflIII 821 ACGTGT A^C[GA][CT]GT
AhdI 256 GACACACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 799 AAATTC [GA]^AATT[CT]
Asp700I 37 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 256 GACACACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 186 GGATCC G^GATCC
BbvI 358 GCAGC GCAGC(8/12)
BclI 897 TGATCA T^GATCA
BpmI 164 CTCCAG CTGGAG(16/14)
BsaAI 820 TACGTG [CT]AC^GT[GA]
BsmFI 853 GTCCC GGGAC(10/14)
BsmI 880 GAATGC GAATGC(1/-1)
Bsp1407I 738 TGTACA T^GTACA
BsrGI 738 TGTACA T^GTACA
BssKI 115 CCTGG ^CC[ACGT]GG
BstNI 115 CCTGG CC^[AT]GG
BstYI 186 GGATCC [GA]^GATC[CT]
Cac8I 386 GCTGGC GC[ACGT]^[ACGT]GC
Eam1105I 256 GACACACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 115 CCTGG ^CC[AT]GG
EcoT22I 882 ATGCAT ATGCA^T
FbaI 897 TGATCA T^GATCA
Fnu4HI 358 GCAGC GC^[ACGT]GC
HgaI 383 GACGC GACGC(5/10)
ItaI 358 GCAGC GC^[ACGT]GC
MfeI 437 CAATTG C^AATTG
MflI 186 GGATCC [GA]^GATC[CT]
MslI 498 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 437 CAATTG C^AATTG
MvaI 115 CCTGG CC^[AT]GG
MwoI 835 GCAGAACTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 882 ATGCAT ATGCA^T
RsaI 739 GTAC GT^AC
ScrFI 115 CCTGG CC^[ACGT]GG
SfcI 546 CTATAG C^T[GA][CT]AG
SspBI 738 TGTACA T^GTACA
SwaI 629 ATTTAAAT ATTT^AAAT
TseI 358 GCAGC G^C[AT]GC
TspRI 182 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 186 GGATCC [GA]^GATC[CT]
XmnI 37 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwI 187 GATCC GGATC(4/5)
186 GGATC
BsaJI 465 CCATGG C^C[ACGT][ACGT]GG
857 CCATGG
DraI 571 TTTAAA TTT^AAA
630 TTTAAA
DsaI 465 CCATGG C^C[GA][CT]GG
857 CCATGG
EcoT14I 465 CCATGG C^C[AT][AT]GG
857 CCATGG
MaeII 821 ACGT A^CGT
866 ACGT
MaeIII 708 GTTAC ^GT[ACGT]AC
775 GTTAC
MnlI 106 GAGG CCTC(7/6)
364 GAGG
NcoI 465 CCATGG C^CATGG
857 CCATGG
NlaIV 162 GGCTCC GG[ACGT]^[ACGT]CC
186 GGATCC
StyI 465 CCATGG C^C[AT][AT]GG
857 CCATGG
TaqI 648 TCGA T^CGA
796 TCGA
TthHB8I 648 TCGA T^CGA
796 TCGA
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AvaII BfaI BsiYI BslI
BsrI BstF5I Cfr13I DdeI DpnII
FokI MaeI MboI MboII MseI
NdeII NlaIII Sau3AI Sau96I Tru9I
Tsp509I