Restriction map for
pF1KB5425
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsgI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaI KasI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp45I Tth111I TthHB8I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AciI 210 CCGC CCGC(-3/-1)
AlwNI 126 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 190 GAGCAC G[AT]GC[AT]^C
AvaII 14 GGACC G^G[AT]CC
BbsI 85 GAAGAC GAAGAC(2/6)
BbvI 339 GCAGC GCAGC(8/12)
BciVI 302 GTATCC GTATCC(6/5)
BfaI 58 CTAG C^TAG
BmyI 190 GAGCAC G[AGT]GC[ACT]^C
BpmI 145 CTGGAG CTGGAG(16/14)
BpuAI 85 GAAGAC GAAGAC(2/6)
BsiHKAI 190 GAGCAC G[AT]GC[AT]^C
BsmFI 13 GGGAC GGGAC(10/14)
Bsp1286I 190 GAGCAC G[AGT]GC[ACT]^C
Cac8I 76 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 14 GGACC G^G[ACGT]CC
DraII 13 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 352 GAAGAG CTCTTC(1/4)
EcoO109I 13 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 209 CCCGC CCCGC(4/6)
Fnu4HI 339 GCAGC GC^[ACGT]GC
HapII 336 CCGG C^CGG
HincII 238 GTTGAC GT[CT]^[GA]AC
HindII 238 GTTGAC GT[CT]^[GA]AC
HpaII 336 CCGG C^CGG
HphI 44 TCACC GGTGA(8/7)
ItaI 339 GCAGC GC^[ACGT]GC
Ksp632I 352 GAAGAG CTCTTC(1/4)
MaeI 58 CTAG C^TAG
MslI 228 CACTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 336 CCGG C^CGG
PpuMI 13 GGGACCC [GA]G^G[AT]CC[CT]
Sau96I 14 GGACC G^G[ACGT]CC
SfcI 243 CTACAG C^T[GA][CT]AG
SmlI 67 CTCAAG C^T[CT][GA]AG
TseI 339 GCAGC G^C[AT]GC
Tsp509I 365 AATT ^AATT
XbaI 57 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AfaI 9 GTAC GT^AC
321 GTAC
BseRI 109 GAGGAG GAGGAG(10/8)
271 GAGGAG
BsrI 121 ACTGG ACTGG(1/-1)
308 CCAGT
BssKI 131 CCTGG ^CC[ACGT]GG
144 CCTGG
BstF5I 90 CATCC GGATG(2/0)
276 GGATG
BstNI 131 CCTGG CC^[AT]GG
144 CCTGG
EcoRII 131 CCTGG ^CC[AT]GG
144 CCTGG
FokI 90 CATCC GGATG(9/13)
276 GGATG
HaeIII 75 GGCC GG^CC
387 GGCC
MvaI 131 CCTGG CC^[AT]GG
144 CCTGG
RsaI 9 GTAC GT^AC
321 GTAC
ScrFI 131 CCTGG CC^[ACGT]GG
144 CCTGG
TspRI 126 CAGTG CA[GC]TG[ACGT][ACGT]^
309 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI BglII BstYI DdeI DpnII
MboI MboII MflI MnlI NdeII
NlaIV Sau3AI XhoII