Restriction map for
pF1KB5403
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AclI AflII ApaI AscI
Asp700I AvrII BalI BbrPI BbvCI
BciVI BfrI BglII BlnI BsiEI
BsiWI BsmI Bsp1407I BspDI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoO65I EcoRI EcoT22I FseI HincII
HindII HindIII HpaI MfeI MluI
MluNI MscI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspBI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 1745 GGTACC G^GTACC
AccI 2053 GTCTAC GT^[AC][GT]AC
AccIII 191 TCCGGA T^CCGGA
AcsI 1360 GAATTT [GA]^AATT[CT]
AgeI 19 ACCGGT A^CCGGT
Alw44I 2452 GTGCAC G^TGCAC
AocI 1576 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1460 AGCGCT AGC^GCT
ApaLI 2452 GTGCAC G^TGCAC
ApoI 1360 GAATTT [GA]^AATT[CT]
AseI 2479 ATTAAT AT^TAAT
AsnI 2479 ATTAAT AT^TAAT
Asp718I 1745 GGTACC G^GTACC
BamHI 1688 GGATCC G^GATCC
BbeI 2000 GGCGCC GGCGC^C
BcgI 489 CGAGCAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 337 CTAG C^TAG
BlpI 111 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC
BsaAI 1405 TACGTG [CT]AC^GT[GA]
BsaBI 1224 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2657 GAGACC GGTCTC(1/5)
BseAI 191 TCCGGA T^CCGGA
BsmBI 2413 GAGACG CGTCTC(1/5)
BspEI 191 TCCGGA T^CCGGA
BspHI 1769 TCATGA T^CATGA
BsrBI 1996 GAGCGG CCGCTC(-3/-3)
BsrFI 19 ACCGGT [GA]^CCGG[CT]
BssSI 1016 CACGAG CACGAG(-5/-1)
Bsu36I 1576 CCTGAGG CC^T[ACGT]AGG
CelII 111 GCTGAGC GC^T[ACGT]AGC
Cfr10I 19 ACCGGT [GA]^CCGG[CT]
Eco47III 1460 AGCGCT AGC^GCT
Eco81I 1576 CCTGAGG CC^T[ACGT]AGG
EcoRV 1228 GATATC GAT^ATC
KasI 2000 GGCGCC G^GCGCC
KpnI 1745 GGTACC GGTAC^C
KspI 2700 CCGCGG CCGC^GG
MaeI 337 CTAG C^TAG
MaeII 1406 ACGT A^CGT
MamI 1224 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 191 TCCGGA T^CCGGA
NarI 2000 GGCGCC GG^CGCC
NcoI 1835 CCATGG C^CATGG
NdeI 266 CATATG CA^TATG
PinAI 19 ACCGGT A^CCGGT
PshBI 2479 ATTAAT AT^TAAT
RcaI 1769 TCATGA T^CATGA
SacII 2700 CCGCGG CCGC^GG
SapI 539 GCTCTTC GCTCTTC(1/4)
SbfI 1962 CCTGCAGG CCTGCA^GG
SexAI 2057 ACCTGGT A^CC[AT]GGT
SfoI 2000 GGCGCC GGC^GCC
SmlI 759 CTCAAG C^T[CT][GA]AG
SphI 1797 GCATGC GCATG^C
Sse8387I 1962 CCTGCAGG CCTGCA^GG
SspI 1267 AATATT AAT^ATT
StuI 1869 AGGCCT AGG^CCT
Tsp509I 1361 AATT ^AATT
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 1105 GGCGTC G[GA]^CG[CT]C
2000 GGCGCC
AhdI 28 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1903 GACATCCAGTC
AspEI 28 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1903 GACATCCAGTC
AvaI 487 CCCGAG C^[CT]CG[GA]G
2308 CCCGAG
AviII 1514 TGCGCA TGC^GCA
1955 TGCGCA
BbsI 685 GAAGAC GAAGAC(2/6)
2247 GAAGAC
BclI 2126 TGATCA T^GATCA
2474 TGATCA
BglI 2218 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2699 GCCGCGGTGGC
BpuAI 685 GAAGAC GAAGAC(2/6)
2247 GAAGAC
BsaHI 1105 GGCGTC G[GA]^CG[CT]C
2000 GGCGCC
BsoBI 487 CCCGAG C^[CT]CG[GA]G
2308 CCCGAG
DraIII 228 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1310 CACTGCGTG
Eam1105I 28 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1903 GACATCCAGTC
FbaI 2126 TGATCA T^GATCA
2474 TGATCA
FspI 1514 TGCGCA TGC^GCA
1955 TGCGCA
Hin1I 1105 GGCGTC G[GA]^CG[CT]C
2000 GGCGCC
PstI 1963 CTGCAG CTGCA^G
2569 CTGCAG
SacI 61 GAGCTC GAGCT^C
250 GAGCTC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AspI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI Tth111I TthHB8I XcmI XhoII