Restriction map for
pF1KE2488
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvrII BamHI BbrPI BbvCI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII ClaI DraI
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MflI MluI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 77 CGCG CG^CG
AccIII 513 TCCGGA T^CCGGA
AcyI 878 GGCGCC G[GA]^CG[CT]C
AocI 39 CCTAAGG CC^T[ACGT]AGG
AviII 788 TGCGCA TGC^GCA
BalI 836 TGGCCA TGG^CCA
BbeI 878 GGCGCC GGCGC^C
BbsI 189 GAAGAC GAAGAC(2/6)
BciVI 3 GGATAC GTATCC(6/5)
BpuAI 189 GAAGAC GAAGAC(2/6)
BsaHI 878 GGCGCC G[GA]^CG[CT]C
BsaI 137 GGTCTC GGTCTC(1/5)
BsaWI 513 TCCGGA [AT]^CCGG[AT]
BseAI 513 TCCGGA T^CCGGA
BspEI 513 TCCGGA T^CCGGA
BstF5I 653 CATCC GGATG(2/0)
BstUI 77 CGCG CG^CG
Bsu36I 39 CCTAAGG CC^T[ACGT]AGG
CpoI 510 CGGTCCG CG^G[AT]CCG
DraIII 792 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 836 TGGCCA [CT]^GGCC[GA]
Eco81I 39 CCTAAGG CC^T[ACGT]AGG
EcoNI 858 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 653 CATCC GGATG(9/13)
FspI 788 TGCGCA TGC^GCA
Hin1I 878 GGCGCC G[GA]^CG[CT]C
KasI 878 GGCGCC G^GCGCC
MluNI 836 TGGCCA TGG^CCA
MroI 513 TCCGGA T^CCGGA
MscI 836 TGGCCA TGG^CCA
MseI 13 TTAA T^TAA
MslI 371 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 77 CGCG CG^CG
NaeI 1065 GCCGGC GCC^GGC
NarI 878 GGCGCC GG^CGCC
NgoMIV 1065 GCCGGC G^CCGGC
NlaIII 577 CATG CATG^
PpuMI 169 GGGTCCC [GA]G^G[AT]CC[CT]
RsrII 510 CGGTCCG CG^G[AT]CCG
ScaI 396 AGTACT AGT^ACT
SfoI 878 GGCGCC GGC^GCC
SmlI 982 CTCAAG C^T[CT][GA]AG
StuI 36 AGGCCT AGG^CCT
Tru9I 13 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 324 GTAC GT^AC
397 GTAC
ApaI 89 GGGCCC GGGCC^C
443 GGGCCC
Asp700I 641 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
753 GAAAAGATTC
BsgI 151 GTGCAG GTGCAG(16/14)
224 GTGCAG
BsmBI 84 GAGACG CGTCTC(1/5)
607 GAGACG
BsrBI 744 CCGCTC CCGCTC(-3/-3)
937 GAGCGG
BsrFI 465 GCCGGT [GA]^CCGG[CT]
1065 GCCGGC
BsrI 399 ACTGG ACTGG(1/-1)
534 ACTGG
Cfr10I 465 GCCGGT [GA]^CCGG[CT]
1065 GCCGGC
EcoT14I 243 CCAAGG C^C[AT][AT]GG
839 CCAAGG
HaeII 878 GGCGCC [GA]GCGC^[CT]
899 AGCGCC
PstI 214 CTGCAG CTGCA^G
1042 CTGCAG
PvuII 599 CAGCTG CAG^CTG
1003 CAGCTG
RsaI 324 GTAC GT^AC
397 GTAC
SfaNI 768 GATGC GCATC(5/9)
453 GCATC
SfcI 214 CTGCAG C^T[GA][CT]AG
1042 CTGCAG
StyI 243 CCAAGG C^C[AT][AT]GG
839 CCAAGG
TaqI 104 TCGA T^CGA
109 TCGA
TthHB8I 104 TCGA T^CGA
109 TCGA
XmnI 641 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
753 GAAAAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIV PleI Sau3AI Sau96I ScrFI
SmaI TfiI TseI Tsp45I XmaCI
XmaI