Restriction map for
pF1KE2680
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwNI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AgeI 1211 ACCGGT A^CCGGT
AocI 1090 CCTGAGG CC^T[ACGT]AGG
BanI 1141 GGCACC G^G[CT][GA]CC
BbsI 289 GTCTTC GAAGAC(2/6)
BcgI 1176 CGAGAATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1153 CTAG C^TAG
BglII 635 AGATCT A^GATCT
BpuAI 289 GTCTTC GAAGAC(2/6)
BsaBI 1047 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1211 ACCGGT [AT]^CCGG[AT]
BsmI 221 GCATTC GAATGC(1/-1)
BspMI 1109 ACCTGC ACCTGC(4/8)
BsrFI 1211 ACCGGT [GA]^CCGG[CT]
BstXI 123 CCACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1090 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1211 ACCGGT [GA]^CCGG[CT]
DraII 508 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 734 CCATGG C^C[GA][CT]GG
Eco81I 1090 CCTGAGG CC^T[ACGT]AGG
EcoO109I 508 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 734 CCATGG C^C[AT][AT]GG
HgaI 422 GCGTC GACGC(5/10)
MaeI 1153 CTAG C^TAG
MamI 1047 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 734 CCATGG C^CATGG
PflMI 495 CCATGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1211 ACCGGT A^CCGGT
PpuMI 508 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 282 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 781 CTGCAG CTGCA^G
PvuII 607 CAGCTG CAG^CTG
SacI 160 GAGCTC GAGCT^C
SapI 49 GAAGAGC GCTCTTC(1/4)
ScaI 788 AGTACT AGT^ACT
SexAI 860 ACCAGGT A^CC[AT]GGT
SmlI 65 CTCAAG C^T[CT][GA]AG
StuI 1149 AGGCCT AGG^CCT
StyI 734 CCATGG C^C[AT][AT]GG
Van91I 495 CCATGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AatII 247 GACGTC GACGT^C
1201 GACGTC
AcyI 247 GACGTC G[GA]^CG[CT]C
1201 GACGTC
AfaI 411 GTAC GT^AC
789 GTAC
Aor51HI 398 AGCGCT AGC^GCT
890 AGCGCT
AspHI 72 GAGCAC G[AT]GC[AT]^C
160 GAGCTC
AvaI 513 CCCGGG C^[CT]CG[GA]G
1124 CCCGGG
BalI 299 TGGCCA TGG^CCA
1022 TGGCCA
BanII 52 GAGCCC G[GA]GC[CT]^C
160 GAGCTC
BpmI 601 CTGGAG CTGGAG(16/14)
850 CTGGAG
BsaHI 247 GACGTC G[GA]^CG[CT]C
1201 GACGTC
BsaI 757 GAGACC GGTCTC(1/5)
448 GGTCTC
BsiHKAI 72 GAGCAC G[AT]GC[AT]^C
160 GAGCTC
BsmAI 757 GAGAC GTCTC(1/5)
449 GTCTC
BsoBI 513 CCCGGG C^[CT]CG[GA]G
1124 CCCGGG
BstAPI 570 GCACTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
774 GCACGGCCTGC
DraIII 571 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1114 CACCGGGTG
EarI 43 GAAGAG CTCTTC(1/4)
49 GAAGAG
Eco47III 398 AGCGCT AGC^GCT
890 AGCGCT
EcoNI 252 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
687 CCTGAGCAAGG
Hin1I 247 GACGTC G[GA]^CG[CT]C
1201 GACGTC
HincII 325 GTCAAC GT[CT]^[GA]AC
1063 GTCAAC
HindII 325 GTCAAC GT[CT]^[GA]AC
1063 GTCAAC
HinfI 1061 GAGTC G^A[ACGT]TC
1096 GACTC
HphI 336 GGTGA GGTGA(8/7)
864 GGTGA
Ksp632I 43 GAAGAG CTCTTC(1/4)
49 GAAGAG
MluNI 299 TGGCCA TGG^CCA
1022 TGGCCA
MscI 299 TGGCCA TGG^CCA
1022 TGGCCA
MslI 735 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1161 CACTTTTGTG
PleI 1096 GACTC GAGTC(4/5)
1061 GAGTC
RsaI 411 GTAC GT^AC
789 GTAC
SfcI 307 CTACAG C^T[GA][CT]AG
781 CTGCAG
SmaI 513 CCCGGG CCC^GGG
1124 CCCGGG
XcmI 233 CCATCCTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
263 CCAAGTCCGGGATGG
XmaCI 513 CCCGGG C^CCGGG
1124 CCCGGG
XmaI 513 CCCGGG C^CCGGG
1124 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI AlwI AvaII BamHI
BbvI BcnI BmyI BsaJI BseRI
BsgI BsiYI BslI BsmFI Bsp1286I
BssKI BssSI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI SfaNI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I XhoII