Restriction map for
pF1KB0963
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 93 CCGC CCGC(-3/-1)
AfaI 51 GTAC GT^AC
ApaI 526 GGGCCC GGGCC^C
BglI 288 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 164 AGATCT A^GATCT
BsgI 67 GTGCAG GTGCAG(16/14)
BstF5I 505 GGATG GGATG(2/0)
BstXI 222 CCAGGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 164 AGATCT [GA]^GATC[CT]
CfoI 227 GCGC GCG^C
DpnII 165 GATC ^GATC
DraII 31 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 249 CCGTGG C^C[GA][CT]GG
EcoNI 35 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 31 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 505 GGATG GGATG(9/13)
HaeII 226 GGCGCT [GA]GCGC^[CT]
HhaI 227 GCGC GCG^C
HinP1I 227 GCGC G^CGC
HphI 487 GGTGA GGTGA(8/7)
MboI 165 GATC ^GATC
MflI 164 AGATCT [GA]^GATC[CT]
MseI 239 TTAA T^TAA
MslI 138 CACAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 93 CCGCTG C[AC]G^C[GT]G
NdeII 165 GATC ^GATC
PleI 453 GAGTC GAGTC(4/5)
RsaI 51 GTAC GT^AC
Sau3AI 165 GATC ^GATC
StuI 295 AGGCCT AGG^CCT
Tru9I 239 TTAA T^TAA
XhoII 164 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 126 AAATTT [GA]^AATT[CT]
322 AAATTT
AluI 85 AGCT AG^CT
402 AGCT
ApoI 126 AAATTT [GA]^AATT[CT]
322 AAATTT
AvaII 44 GGACC G^G[AT]CC
246 GGACC
BalI 219 TGGCCA TGG^CCA
546 TGGCCA
BanII 526 GGGCCC G[GA]GC[CT]^C
560 GGGCTC
BmyI 526 GGGCCC G[AGT]GC[ACT]^C
560 GGGCTC
Bsp1286I 526 GGGCCC G[AGT]GC[ACT]^C
560 GGGCTC
BssKI 222 CCAGG ^CC[ACGT]GG
413 CCTGG
BstAPI 110 GCATGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
173 GCAAATCCTGC
BstNI 222 CCAGG CC^[AT]GG
413 CCTGG
Cac8I 229 GCTGGC GC[ACGT]^[ACGT]GC
233 GCACGC
DdeI 329 CTCAG C^T[ACGT]AG
347 CTGAG
EaeI 219 TGGCCA [CT]^GGCC[GA]
546 TGGCCA
EcoRII 222 CCAGG ^CC[AT]GG
413 CCTGG
EcoT14I 530 CCAAGG C^C[AT][AT]GG
549 CCAAGG
MluNI 219 TGGCCA TGG^CCA
546 TGGCCA
MscI 219 TGGCCA TGG^CCA
546 TGGCCA
MvaI 222 CCAGG CC^[AT]GG
413 CCTGG
NlaIV 31 GGGGCC GG[ACGT]^[ACGT]CC
526 GGGCCC
ScrFI 222 CCAGG CC^[ACGT]GG
413 CCTGG
SfaNI 506 GATGC GCATC(5/9)
266 GCATC
StyI 530 CCAAGG C^C[AT][AT]GG
549 CCAAGG
TfiI 370 GATTC G^A[AT]TC
460 GAATC
TspRI 70 CAGTG CA[GC]TG[ACGT][ACGT]^
143 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI Cfr13I
EarI Fnu4HI HaeIII HinfI ItaI
Ksp632I MboII MnlI MwoI NlaIII
Sau96I TseI Tsp509I