Restriction map for
pF1KE2300
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaHI BsaI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Cac8I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
FauI FbaI FseI FspI HgaI
Hin1I KasI KpnI KspI MluI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 1227 TCCGGA T^CCGGA
AocI 2156 CCTTAGG CC^T[ACGT]AGG
Aor51HI 288 AGCGCT AGC^GCT
AseI 2212 ATTAAT AT^TAAT
AsnI 2212 ATTAAT AT^TAAT
AspHI 2350 GAGCTC G[AT]GC[AT]^C
AvaI 144 CTCGGG C^[CT]CG[GA]G
BanII 2350 GAGCTC G[GA]GC[CT]^C
BbrPI 383 CACGTG CAC^GTG
BglII 2286 AGATCT A^GATCT
BmyI 2350 GAGCTC G[AGT]GC[ACT]^C
BsaAI 383 CACGTG [CT]AC^GT[GA]
BsaBI 2245 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1227 TCCGGA T^CCGGA
BseRI 589 GAGGAG GAGGAG(10/8)
BsiHKAI 2350 GAGCTC G[AT]GC[AT]^C
BsiYI 918 CCGAGATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 918 CCGAGATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 144 CTCGGG C^[CT]CG[GA]G
Bsp1286I 2350 GAGCTC G[AGT]GC[ACT]^C
BspEI 1227 TCCGGA T^CCGGA
BspHI 2253 TCATGA T^CATGA
BstEII 760 GGTTACC G^GT[ACGT]ACC
BstPI 760 GGTTACC G^GT[ACGT]ACC
Bsu36I 2156 CCTTAGG CC^T[ACGT]AGG
CfoI 289 GCGC GCG^C
DraII 2321 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1861 CCATGG C^C[GA][CT]GG
EarI 815 GAAGAG CTCTTC(1/4)
Eco47III 288 AGCGCT AGC^GCT
Eco81I 2156 CCTTAGG CC^T[ACGT]AGG
EcoO109I 2321 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 760 GGTTACC G^GT[ACGT]ACC
EcoRV 2337 GATATC GAT^ATC
EcoT22I 1548 ATGCAT ATGCA^T
HaeII 288 AGCGCT [GA]GCGC^[CT]
HhaI 289 GCGC GCG^C
HinP1I 289 GCGC G^CGC
HindIII 108 AAGCTT A^AGCTT
Ksp632I 815 GAAGAG CTCTTC(1/4)
MamI 2245 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1227 TCCGGA T^CCGGA
MspA1I 577 CAGCTG C[AC]G^C[GT]G
NcoI 1861 CCATGG C^CATGG
NsiI 1548 ATGCAT ATGCA^T
PleI 818 GAGTC GAGTC(4/5)
PmaCI 383 CACGTG CAC^GTG
PmlI 383 CACGTG CAC^GTG
PpuMI 2321 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2212 ATTAAT AT^TAAT
PvuII 577 CAGCTG CAG^CTG
RcaI 2253 TCATGA T^CATGA
SacI 2350 GAGCTC GAGCT^C
SspI 1816 AATATT AAT^ATT
XbaI 904 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 232 CCGC CCGC(-3/-1)
552 GCGG
BpmI 1403 CTCCAG CTGGAG(16/14)
2100 CTCCAG
BsmAI 62 GAGAC GTCTC(1/5)
777 GAGAC
BsmFI 2321 GGGAC GGGAC(10/14)
820 GTCCC
BspMI 1428 ACCTGC ACCTGC(4/8)
1461 ACCTGC
BstYI 937 GGATCT [GA]^GATC[CT]
2286 AGATCT
HphI 2515 GGTGA GGTGA(8/7)
1306 TCACC
MaeII 384 ACGT A^CGT
513 ACGT
MfeI 35 CAATTG C^AATTG
281 CAATTG
MflI 937 GGATCT [GA]^GATC[CT]
2286 AGATCT
MunI 35 CAATTG C^AATTG
281 CAATTG
MwoI 280 GCAATTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1476 GCAGCAACAGC
NspI 734 ACATGC [GA]CATG^[CT]
2232 ACATGC
XhoII 937 GGATCT [GA]^GATC[CT]
2286 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BalI BbsI BbvI
BfaI BpuAI BsaJI BsaWI BsmI
BsrI BssKI BstF5I BstNI BstXI
Cfr13I DdeI DpnII DraI EaeI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaI HpaII ItaI MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI