Restriction map for
pF1KB5481
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII ClaI CpoI
DpnII DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MboI MfeI MflI
MluI MroI MslI MunI NarI
NciI NcoI NdeI NdeII NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 831 CGCG CG^CG
AflIII 891 ACATGT A^C[GA][CT]GT
AocI 103 CCTCAGG CC^T[ACGT]AGG
AspHI 89 GAGCAC G[AT]GC[AT]^C
AvaI 30 CTCGAG C^[CT]CG[GA]G
BalI 1299 TGGCCA TGG^CCA
BbsI 1161 GTCTTC GAAGAC(2/6)
BbvCI 466 CCTCAGC CCTCAGC(-5/-2)
BciVI 1141 GGATAC GTATCC(6/5)
BfaI 1201 CTAG C^TAG
BmyI 89 GAGCAC G[AGT]GC[ACT]^C
BpmI 1040 CTGGAG CTGGAG(16/14)
BpuAI 1161 GTCTTC GAAGAC(2/6)
BsaWI 545 ACCGGA [AT]^CCGG[AT]
BseRI 607 GAGGAG GAGGAG(10/8)
BsiHKAI 89 GAGCAC G[AT]GC[AT]^C
BsiYI 338 CCGCAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 338 CCGCAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 788 GAGAC GTCTC(1/5)
BsoBI 30 CTCGAG C^[CT]CG[GA]G
Bsp1286I 89 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 891 ACATGT A^CATGT
BsrFI 19 GCCGGC [GA]^CCGG[CT]
BstUI 831 CGCG CG^CG
Bsu36I 103 CCTCAGG CC^T[ACGT]AGG
CfoI 79 GCGC GCG^C
Cfr10I 19 GCCGGC [GA]^CCGG[CT]
DrdI 790 GACAGTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1299 TGGCCA [CT]^GGCC[GA]
Eco81I 103 CCTCAGG CC^T[ACGT]AGG
EcoRV 1267 GATATC GAT^ATC
EcoT22I 851 ATGCAT ATGCA^T
HgaI 249 GCGTC GACGC(5/10)
HhaI 79 GCGC GCG^C
HinP1I 79 GCGC G^CGC
MaeI 1201 CTAG C^TAG
MluNI 1299 TGGCCA TGG^CCA
MscI 1299 TGGCCA TGG^CCA
MvnI 831 CGCG CG^CG
NaeI 19 GCCGGC GCC^GGC
NgoMIV 19 GCCGGC G^CCGGC
NsiI 851 ATGCAT ATGCA^T
PaeR7I 30 CTCGAG C^TCGAG
PshAI 510 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 83 CTGCAG CTGCA^G
SbfI 82 CCTGCAGG CCTGCA^GG
SphI 531 GCATGC GCATG^C
Sse8387I 82 CCTGCAGG CCTGCA^GG
TaqI 31 TCGA T^CGA
TthHB8I 31 TCGA T^CGA
XhoI 30 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 992 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
1034 CAGCTGCTG
AvaII 108 GGACC G^G[AT]CC
1190 GGTCC
BsgI 925 CTGCAC GTGCAG(16/14)
839 GTGCAG
BsmFI 1332 GGGAC GGGAC(10/14)
1191 GTCCC
BsmI 370 GAATGC GAATGC(1/-1)
549 GAATGC
BsrI 260 CCAGT ACTGG(1/-1)
983 CCAGT
BstAPI 817 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
973 GCATTTAATGC
DraII 107 AGGACCT [GA]G^G[ACGT]CC[CT]
1189 GGGTCCC
DsaI 300 CCGTGG C^C[GA][CT]GG
759 CCGTGG
EcoO109I 107 AGGACCT [GA]G^G[ACGT]CC[CT]
1189 GGGTCCC
EcoT14I 554 CCTTGG C^C[AT][AT]GG
941 CCAAGG
FauI 378 GCGGG CCCGC(4/6)
832 GCGGG
HapII 20 CCGG C^CGG
546 CCGG
HpaII 20 CCGG C^CGG
546 CCGG
MspA1I 74 CAGCTG C[AC]G^C[GT]G
1034 CAGCTG
MspI 20 CCGG C^CGG
546 CCGG
PpuMI 107 AGGACCT [GA]G^G[AT]CC[CT]
1189 GGGTCCC
PvuII 74 CAGCTG CAG^CTG
1034 CAGCTG
SfcI 83 CTGCAG C^T[GA][CT]AG
582 CTATAG
StyI 554 CCTTGG C^C[AT][AT]GG
941 CCAAGG
TspRI 1154 CAGTG CA[GC]TG[ACGT][ACGT]^
1253 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
BbvI BsaJI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII NlaIV NspI PleI
Sau96I ScrFI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I XmnI