Restriction map for
pF1KB6750
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I EarI
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MamI MboII MfeI MluI
MroI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I Tsp45I
Tsp509I TspRI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 242 GACGTC GACGT^C
AfaI 192 GTAC GT^AC
AocI 373 CCTCAGG CC^T[ACGT]AGG
Asp700I 150 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 47 GAGCTC G[AT]GC[AT]^C
BalI 116 TGGCCA TGG^CCA
BanI 185 GGCACC G^G[CT][GA]CC
BanII 47 GAGCTC G[GA]GC[CT]^C
BciVI 102 GGATAC GTATCC(6/5)
BmyI 47 GAGCTC G[AGT]GC[ACT]^C
BsiEI 357 CGGCCG CG[GA][CT]^CG
BsiHKAI 47 GAGCTC G[AT]GC[AT]^C
Bsp1286I 47 GAGCTC G[AGT]GC[ACT]^C
BsrI 152 ACTGG ACTGG(1/-1)
BstXI 287 CCAAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 373 CCTCAGG CC^T[ACGT]AGG
DdeI 374 CTCAG C^T[ACGT]AG
DraII 305 GGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 357 CGGCCG C^GGCCG
EclXI 357 CGGCCG C^GGCCG
Eco52I 357 CGGCCG C^GGCCG
Eco81I 373 CCTCAGG CC^T[ACGT]AGG
EcoO109I 305 GGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 299 CCCGC CCCGC(4/6)
HaeII 205 GGCGCT [GA]GCGC^[CT]
HgaI 257 GCGTC GACGC(5/10)
HinfI 398 GAATC G^A[ACGT]TC
MaeIII 10 GTTAC ^GT[ACGT]AC
MluNI 116 TGGCCA TGG^CCA
MscI 116 TGGCCA TGG^CCA
MspA1I 416 CAGCTG C[AC]G^C[GT]G
NotI 356 GCGGCCGC GC^GGCCGC
PflMI 312 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 305 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 416 CAGCTG CAG^CTG
RsaI 192 GTAC GT^AC
SacI 47 GAGCTC GAGCT^C
TfiI 398 GAATC G^A[AT]TC
Van91I 312 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 150 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 164 CGCG CG^CG
182 CGCG
AcyI 242 GACGTC G[GA]^CG[CT]C
256 GGCGTC
AvaII 306 GGTCC G^G[AT]CC
345 GGACC
BglII 230 AGATCT A^GATCT
387 AGATCT
BpmI 5 CTGGAG CTGGAG(16/14)
319 CTGGAG
BsaHI 242 GACGTC G[GA]^CG[CT]C
256 GGCGTC
BstNI 4 CCTGG CC^[AT]GG
318 CCTGG
BstUI 164 CGCG CG^CG
182 CGCG
BstYI 230 AGATCT [GA]^GATC[CT]
387 AGATCT
CfoI 181 GCGC GCG^C
206 GCGC
DpnII 231 GATC ^GATC
388 GATC
EaeI 116 TGGCCA [CT]^GGCC[GA]
357 CGGCCG
EcoRII 4 CCTGG ^CC[AT]GG
318 CCTGG
HhaI 181 GCGC GCG^C
206 GCGC
Hin1I 242 GACGTC G[GA]^CG[CT]C
256 GGCGTC
HinP1I 181 GCGC G^CGC
206 GCGC
MaeII 23 ACGT A^CGT
243 ACGT
MboI 231 GATC ^GATC
388 GATC
MflI 230 AGATCT [GA]^GATC[CT]
387 AGATCT
MvaI 4 CCTGG CC^[AT]GG
318 CCTGG
MvnI 164 CGCG CG^CG
182 CGCG
NdeII 231 GATC ^GATC
388 GATC
NlaIII 222 CATG CATG^
261 CATG
Sau3AI 231 GATC ^GATC
388 GATC
XhoII 230 AGATCT [GA]^GATC[CT]
387 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BsmFI BssKI Cac8I
Cfr13I Fnu4HI HaeIII HapII HpaII
ItaI MnlI MspI MwoI NciI
NlaIV Sau96I ScrFI TseI