Restriction map for
pF1KE0416
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BsmFI BsmI
BspDI BspHI BspMI BsrDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI StyI SwaI
Tth111I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 982 CGCG CG^CG
AccIII 776 TCCGGA T^CCGGA
AflIII 751 ACATGT A^C[GA][CT]GT
AlwI 444 GGATC GGATC(4/5)
AocI 874 CCTGAGG CC^T[ACGT]AGG
BfaI 454 CTAG C^TAG
BlpI 858 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 858 GCTGAGC GC^T[ACGT]AGC
BsaWI 776 TCCGGA [AT]^CCGG[AT]
BseAI 776 TCCGGA T^CCGGA
BsgI 966 CTGCAC GTGCAG(16/14)
Bsp1407I 1046 TGTACA T^GTACA
BspEI 776 TCCGGA T^CCGGA
BspLU11I 751 ACATGT A^CATGT
BsrFI 86 ACCGGC [GA]^CCGG[CT]
BsrGI 1046 TGTACA T^GTACA
BssSI 391 CACGAG CACGAG(-5/-1)
BstEII 60 GGTCACC G^GT[ACGT]ACC
BstPI 60 GGTCACC G^GT[ACGT]ACC
BstUI 982 CGCG CG^CG
BstYI 444 GGATCT [GA]^GATC[CT]
Bsu36I 874 CCTGAGG CC^T[ACGT]AGG
CelII 858 GCTGAGC GC^T[ACGT]AGC
Cfr10I 86 ACCGGC [GA]^CCGG[CT]
DraI 150 TTTAAA TTT^AAA
DraIII 969 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 510 CGGCCA [CT]^GGCC[GA]
Eco81I 874 CCTGAGG CC^T[ACGT]AGG
EcoO65I 60 GGTCACC G^GT[ACGT]ACC
MaeI 454 CTAG C^TAG
MaeII 956 ACGT A^CGT
MaeIII 61 GTCAC ^GT[ACGT]AC
MflI 444 GGATCT [GA]^GATC[CT]
MroI 776 TCCGGA T^CCGGA
MvnI 982 CGCG CG^CG
PflMI 243 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 853 CAGCTG CAG^CTG
SacI 112 GAGCTC GAGCT^C
SapI 894 GAAGAGC GCTCTTC(1/4)
ScaI 653 AGTACT AGT^ACT
SexAI 380 ACCTGGT A^CC[AT]GGT
SphI 647 GCATGC GCATG^C
SspBI 1046 TGTACA T^GTACA
Tsp45I 61 GTCAC ^GT[GC]AC
Tsp509I 270 AATT ^AATT
Van91I 243 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 453 TCTAGA T^CTAGA
XhoII 444 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 112 GAGCTC G[AT]GC[AT]^C
292 GAGCAC
AvaI 98 CCCGGG C^[CT]CG[GA]G
1031 CCCGGG
AvaII 359 GGACC G^G[AT]CC
603 GGTCC
BanI 46 GGCACC G^G[CT][GA]CC
384 GGTGCC
BanII 112 GAGCTC G[GA]GC[CT]^C
994 GAGCCC
BpmI 173 CTCCAG CTGGAG(16/14)
943 CTGGAG
BsaI 550 GAGACC GGTCTC(1/5)
102 GGTCTC
BseRI 556 GAGGAG GAGGAG(10/8)
829 GAGGAG
BsiHKAI 112 GAGCTC G[AT]GC[AT]^C
292 GAGCAC
BsoBI 98 CCCGGG C^[CT]CG[GA]G
1031 CCCGGG
BsrBI 6 GAGCGG CCGCTC(-3/-3)
529 GAGCGG
BstAPI 783 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
968 GCACCGAGTGC
BstXI 50 CCACCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCACGAGGATGG
EarI 763 CTCTTC CTCTTC(1/4)
894 GAAGAG
HgaI 707 GCGTC GACGC(5/10)
935 GCGTC
Ksp632I 763 CTCTTC CTCTTC(1/4)
894 GAAGAG
NspI 647 GCATGC [GA]CATG^[CT]
751 ACATGT
SmaI 98 CCCGGG CCC^GGG
1031 CCCGGG
SmlI 115 CTCAAG C^T[CT][GA]AG
814 CTCAAG
TfiI 309 GAATC G^A[AT]TC
486 GATTC
TspRI 368 CAGTG CA[GC]TG[ACGT][ACGT]^
440 CAGTG
XmaCI 98 CCCGGG C^CCGGG
1031 CCCGGG
XmaI 98 CCCGGG C^CCGGG
1031 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwNI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
Tru9I TseI TthHB8I