Restriction map for
pF1KE0005
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AvaI AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BbvI BcgI
BcnI BfrI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CelII Cfr10I
CpoI DraII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoRV EcoT14I EcoT22I FauI Fnu4HI
FseI HaeII HapII HgaI Hin1I
HindIII HpaI HpaII ItaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StyI SwaI TseI
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1838 GGTACC G^GTACC
AciI 1518 CCGC CCGC(-3/-1)
AflIII 1873 ACATGT A^C[GA][CT]GT
AocI 1224 CCTGAGG CC^T[ACGT]AGG
Asp718I 1838 GGTACC G^GTACC
AspI 1148 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1288 GGACC G^G[AT]CC
AviII 1577 TGCGCA TGC^GCA
BanI 1838 GGTACC G^G[CT][GA]CC
BclI 54 TGATCA T^GATCA
BglII 219 AGATCT A^GATCT
BpmI 1061 CTCCAG CTGGAG(16/14)
BsaJI 1532 CCGAGG C^C[ACGT][ACGT]GG
BseRI 1744 GAGGAG GAGGAG(10/8)
BsgI 1663 GTGCAG GTGCAG(16/14)
BsmFI 126 GGGAC GGGAC(10/14)
BsmI 880 GAATGC GAATGC(1/-1)
BspDI 148 ATCGAT AT^CGAT
BspLU11I 1873 ACATGT A^CATGT
BsrI 1789 CCAGT ACTGG(1/-1)
BstEII 289 GGTGACC G^GT[ACGT]ACC
BstPI 289 GGTGACC G^GT[ACGT]ACC
BstYI 219 AGATCT [GA]^GATC[CT]
Bsu36I 1224 CCTGAGG CC^T[ACGT]AGG
Cac8I 1003 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 1578 GCGC GCG^C
Cfr13I 1288 GGACC G^G[ACGT]CC
ClaI 148 ATCGAT AT^CGAT
DraIII 89 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1978 GACATTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1224 CCTGAGG CC^T[ACGT]AGG
EcoNI 918 CCTACGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 289 GGTGACC G^GT[ACGT]ACC
FbaI 54 TGATCA T^GATCA
FspI 1577 TGCGCA TGC^GCA
HhaI 1578 GCGC GCG^C
HinP1I 1578 GCGC G^CGC
HincII 745 GTCAAC GT[CT]^[GA]AC
HindII 745 GTCAAC GT[CT]^[GA]AC
KpnI 1838 GGTACC GGTAC^C
MflI 219 AGATCT [GA]^GATC[CT]
NspI 1873 ACATGT [GA]CATG^[CT]
PflFI 1148 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1059 GACTC GAGTC(4/5)
Sau96I 1288 GGACC G^G[ACGT]CC
ScaI 1791 AGTACT AGT^ACT
SmlI 184 CTTGAG C^T[CT][GA]AG
TaqI 149 TCGA T^CGA
Tth111I 1148 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 149 TCGA T^CGA
XhoII 219 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 1405 GATCC GGATC(4/5)
1750 GGATC
BbsI 1393 GAAGAC GAAGAC(2/6)
1690 GAAGAC
BciVI 1798 GGATAC GTATCC(6/5)
1544 GTATCC
BpuAI 1393 GAAGAC GAAGAC(2/6)
1690 GAAGAC
BsmAI 1146 GAGAC GTCTC(1/5)
2025 GTCTC
BspHI 399 TCATGA T^CATGA
1974 TCATGA
BsrDI 1511 CATTGC GCAATG(2/0)
1039 GCAATG
EarI 634 GAAGAG CTCTTC(1/4)
1741 GAAGAG
EcoRI 478 GAATTC G^AATTC
1477 GAATTC
HaeIII 869 GGCC GG^CC
1006 GGCC
Ksp632I 634 GAAGAG CTCTTC(1/4)
1741 GAAGAG
MslI 853 CACAAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2005 CACATTGATG
NlaIV 1838 GGTACC GG[ACGT]^[ACGT]CC
1999 GGTTCC
PflMI 1789 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1959 CCAACTAATGG
RcaI 399 TCATGA T^CATGA
1974 TCATGA
SexAI 1730 ACCTGGT A^CC[AT]GGT
1911 ACCAGGT
StuI 868 AGGCCT AGG^CCT
1005 AGGCCT
Van91I 1789 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1959 CCAACTAATGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
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AcsI AfaI AluI ApoI BfaI
BsiYI BslI BssKI BstF5I BstNI
DdeI DpnII DraI EcoRII FokI
HinfI HphI MaeI MaeII MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII RsaI Sau3AI ScrFI
SfaNI SfcI SspI TfiI Tru9I
Tsp45I Tsp509I TspRI