Restriction map for
pF1KB5207
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI AlwNI AocI ApaI
AscI AseI AsnI AspEI AviII
AvrII BanII BbrPI BcgI BciVI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
HindIII HpaI KspI MamI MfeI
MluI MroI MunI NaeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SfuI SgrAI
SmlI SnaBI SpeI SphI SspI
SwaI XhoI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AatII 214 GACGTC GACGT^C
Acc65I 1443 GGTACC G^GTACC
AccII 1157 CGCG CG^CG
Aor51HI 795 AGCGCT AGC^GCT
Asp700I 971 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1443 GGTACC G^GTACC
AspI 1356 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 839 CCCGGG C^[CT]CG[GA]G
BalI 1004 TGGCCA TGG^CCA
BbeI 169 GGCGCC GGCGC^C
BclI 644 TGATCA T^GATCA
BglI 37 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI 1578 GCATTC GAATGC(1/-1)
BsoBI 839 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1068 TGTACA T^GTACA
BspHI 431 TCATGA T^CATGA
BspMI 1486 GCAGGT ACCTGC(4/8)
BsrBI 104 CCGCTC CCGCTC(-3/-3)
BsrGI 1068 TGTACA T^GTACA
BssSI 833 CTCGTG CACGAG(-5/-1)
BstUI 1157 CGCG CG^CG
BstXI 1007 CCACTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 824 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1573 GAAGAG CTCTTC(1/4)
Eco47III 795 AGCGCT AGC^GCT
EcoRV 1306 GATATC GAT^ATC
FauI 99 GCGGG CCCGC(4/6)
FbaI 644 TGATCA T^GATCA
HgaI 70 GCGTC GACGC(5/10)
HincII 1353 GTTGAC GT[CT]^[GA]AC
HindII 1353 GTTGAC GT[CT]^[GA]AC
KasI 169 GGCGCC G^GCGCC
KpnI 1443 GGTACC GGTAC^C
Ksp632I 1573 GAAGAG CTCTTC(1/4)
MluNI 1004 TGGCCA TGG^CCA
MscI 1004 TGGCCA TGG^CCA
MvnI 1157 CGCG CG^CG
NarI 169 GGCGCC GG^CGCC
NcoI 678 CCATGG C^CATGG
NdeI 1429 CATATG CA^TATG
NheI 576 GCTAGC G^CTAGC
PflFI 1356 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 300 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 111 GACGGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1282 CTGCAG CTGCA^G
PvuII 1187 CAGCTG CAG^CTG
RcaI 431 TCATGA T^CATGA
SapI 1573 GAAGAGC GCTCTTC(1/4)
SbfI 1281 CCTGCAGG CCTGCA^GG
ScaI 372 AGTACT AGT^ACT
SexAI 825 ACCTGGT A^CC[AT]GGT
SfcI 1282 CTGCAG C^T[GA][CT]AG
SfiI 36 GGCCTCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 169 GGCGCC GGC^GCC
SmaI 839 CCCGGG CCC^GGG
Sse8387I 1281 CCTGCAGG CCTGCA^GG
SspBI 1068 TGTACA T^GTACA
StuI 1514 AGGCCT AGG^CCT
TaqI 569 TCGA T^CGA
Tsp45I 550 GTGAC ^GT[GC]AC
Tth111I 1356 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 569 TCGA T^CGA
Van91I 300 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1200 TCTAGA T^CTAGA
XmaCI 839 CCCGGG C^CCGGG
XmaI 839 CCCGGG C^CCGGG
XmnI 971 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 398 AAATTC [GA]^AATT[CT]
542 AAATTC
AcyI 169 GGCGCC G[GA]^CG[CT]C
214 GACGTC
Alw44I 386 GTGCAC G^TGCAC
1367 GTGCAC
ApaLI 386 GTGCAC G^TGCAC
1367 GTGCAC
ApoI 398 AAATTC [GA]^AATT[CT]
542 AAATTC
BamHI 1194 GGATCC G^GATCC
1224 GGATCC
BanI 169 GGCGCC G^G[CT][GA]CC
1443 GGTACC
BbsI 1345 GAAGAC GAAGAC(2/6)
1417 GTCTTC
BbvCI 502 GCTGAGG CCTCAGC(-5/-2)
1411 GCTGAGG
BbvI 28 GCTGC GCAGC(8/12)
75 GCTGC
BfaI 577 CTAG C^TAG
1201 CTAG
BlpI 85 GCTCAGC GC^T[ACGT]AGC
1168 GCTGAGC
Bpu1102I 85 GCTCAGC GC^T[ACGT]AGC
1168 GCTGAGC
BpuAI 1345 GAAGAC GAAGAC(2/6)
1417 GTCTTC
BsaHI 169 GGCGCC G[GA]^CG[CT]C
214 GACGTC
CelII 85 GCTCAGC GC^T[ACGT]AGC
1168 GCTGAGC
DsaI 678 CCATGG C^C[GA][CT]GG
1263 CCGTGG
EaeI 23 TGGCCG [CT]^GGCC[GA]
1004 TGGCCA
EcoT14I 590 CCAAGG C^C[AT][AT]GG
678 CCATGG
HaeII 169 GGCGCC [GA]GCGC^[CT]
795 AGCGCT
Hin1I 169 GGCGCC G[GA]^CG[CT]C
214 GACGTC
MaeI 577 CTAG C^TAG
1201 CTAG
MaeII 215 ACGT A^CGT
1123 ACGT
MaeIII 550 GTGAC ^GT[ACGT]AC
686 GTTAC
MseI 655 TTAA T^TAA
772 TTAA
MspA1I 26 CCGCTG C[AC]G^C[GT]G
1187 CAGCTG
PleI 56 GACTC GAGTC(4/5)
1204 GACTC
StyI 590 CCAAGG C^C[AT][AT]GG
678 CCATGG
Tru9I 655 TTAA T^TAA
772 TTAA
TseI 28 GCTGC G^C[AT]GC
75 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BcnI BmyI BpmI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MboII MflI MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tsp509I TspRI XcmI XhoII