Restriction map for
pF1KB5155
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MaeII MamI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1473 GTCTAC GT^[AC][GT]AC
AccII 58 CGCG CG^CG
AcsI 1225 AAATTC [GA]^AATT[CT]
ApoI 1225 AAATTC [GA]^AATT[CT]
BanI 1396 GGTGCC G^G[CT][GA]CC
BbvCI 846 CCTCAGC CCTCAGC(-5/-2)
BcnI 99 CCGGG CC^[GC]GG
BfaI 298 CTAG C^TAG
BglI 163 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 628 CTGGAG CTGGAG(16/14)
BsgI 772 GTGCAG GTGCAG(16/14)
BsmAI 1195 GAGAC GTCTC(1/5)
BsmI 493 GCATTC GAATGC(1/-1)
BspMI 719 ACCTGC ACCTGC(4/8)
BstUI 58 CGCG CG^CG
DraI 1222 TTTAAA TTT^AAA
DsaI 1000 CCATGG C^C[GA][CT]GG
EcoRV 370 GATATC GAT^ATC
FauI 279 GCGGG CCCGC(4/6)
HapII 99 CCGG C^CGG
HgaI 1177 GCGTC GACGC(5/10)
HincII 1363 GTCAAC GT[CT]^[GA]AC
HindII 1363 GTCAAC GT[CT]^[GA]AC
HindIII 303 AAGCTT A^AGCTT
HpaII 99 CCGG C^CGG
HphI 349 GGTGA GGTGA(8/7)
MaeI 298 CTAG C^TAG
MfeI 1151 CAATTG C^AATTG
MspA1I 890 CAGCTG C[AC]G^C[GT]G
MspI 99 CCGG C^CGG
MunI 1151 CAATTG C^AATTG
MvnI 58 CGCG CG^CG
NciI 99 CCGGG CC^[GC]GG
NcoI 1000 CCATGG C^CATGG
NspI 1052 GCATGT [GA]CATG^[CT]
PflMI 1372 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 890 CAGCTG CAG^CTG
SacI 572 GAGCTC GAGCT^C
ScaI 294 AGTACT AGT^ACT
SfcI 1491 CTACAG C^T[GA][CT]AG
Van91I 1372 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 57 CCGC CCGC(-3/-1)
279 GCGG
AspHI 572 GAGCTC G[AT]GC[AT]^C
738 GAGCAC
BanII 52 GGGCTC G[GA]GC[CT]^C
572 GAGCTC
BbvI 642 GCTGC GCAGC(8/12)
877 GCTGC
BglII 433 AGATCT A^GATCT
1302 AGATCT
BsiHKAI 572 GAGCTC G[AT]GC[AT]^C
738 GAGCAC
BsmFI 1004 GGGAC GGGAC(10/14)
1275 GGGAC
BsrDI 1182 CATTGC GCAATG(2/0)
694 GCAATG
BstF5I 939 CATCC GGATG(2/0)
401 GGATG
BstXI 620 CCAAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1298 CCAAAGATCTGG
DraII 411 AGGACCT [GA]G^G[ACGT]CC[CT]
1004 GGGACCC
EarI 871 CTCTTC CTCTTC(1/4)
1240 GAAGAG
EcoO109I 411 AGGACCT [GA]G^G[ACGT]CC[CT]
1004 GGGACCC
Fnu4HI 642 GCTGC GC^[ACGT]GC
877 GCTGC
FokI 939 CATCC GGATG(9/13)
401 GGATG
ItaI 642 GCTGC GC^[ACGT]GC
877 GCTGC
Ksp632I 871 CTCTTC CTCTTC(1/4)
1240 GAAGAG
MseI 965 TTAA T^TAA
1223 TTAA
PleI 152 GAGTC GAGTC(4/5)
1435 GAGTC
PpuMI 411 AGGACCT [GA]G^G[AT]CC[CT]
1004 GGGACCC
StuI 471 AGGCCT AGG^CCT
971 AGGCCT
Tru9I 965 TTAA T^TAA
1223 TTAA
TseI 642 GCTGC G^C[AT]GC
877 GCTGC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AlwI AvaII BalI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BsrI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I HaeIII HinfI MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tsp45I
Tsp509I TspRI XhoII