Restriction map for
pF1KB3238
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiWI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AflIII 1298 ACATGT A^C[GA][CT]GT
Alw44I 235 GTGCAC G^TGCAC
Aor51HI 1958 AGCGCT AGC^GCT
ApaLI 235 GTGCAC G^TGCAC
Asp700I 1011 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 235 GTGCAC G[AT]GC[AT]^C
AvaI 1502 CCCGGG C^[CT]CG[GA]G
AvaII 1681 GGACC G^G[AT]CC
BanII 1718 GAGCCC G[GA]GC[CT]^C
BciVI 1872 GGATAC GTATCC(6/5)
BclI 2054 TGATCA T^GATCA
BfaI 1801 CTAG C^TAG
BsaBI 214 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 533 GAGGAG GAGGAG(10/8)
BsiEI 7 CGGTCG CG[GA][CT]^CG
BsiHKAI 235 GTGCAC G[AT]GC[AT]^C
BsmBI 1731 CGTCTC CGTCTC(1/5)
BsmFI 527 GGGAC GGGAC(10/14)
BsoBI 1502 CCCGGG C^[CT]CG[GA]G
Bsp1407I 123 TGTACA T^GTACA
BspDI 795 ATCGAT AT^CGAT
BspLU11I 1298 ACATGT A^CATGT
BsrGI 123 TGTACA T^GTACA
BstAPI 1904 GCATAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 1819 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 1959 GCGC GCG^C
ClaI 795 ATCGAT AT^CGAT
DraI 915 TTTAAA TTT^AAA
DsaI 561 CCATGG C^C[GA][CT]GG
Eco47III 1958 AGCGCT AGC^GCT
EcoRV 636 GATATC GAT^ATC
EcoT14I 561 CCATGG C^C[AT][AT]GG
FauI 1555 CCCGC CCCGC(4/6)
FbaI 2054 TGATCA T^GATCA
HaeII 1958 AGCGCT [GA]GCGC^[CT]
HhaI 1959 GCGC GCG^C
HinP1I 1959 GCGC G^CGC
MaeI 1801 CTAG C^TAG
MaeII 44 ACGT A^CGT
MamI 214 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 561 CCATGG C^CATGG
NspI 1298 ACATGT [GA]CATG^[CT]
PflMI 731 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1118 CTGCAG CTGCA^G
SexAI 297 ACCAGGT A^CC[AT]GGT
SmaI 1502 CCCGGG CCC^GGG
SspBI 123 TGTACA T^GTACA
SspI 399 AATATT AAT^ATT
StyI 561 CCATGG C^C[AT][AT]GG
Van91I 731 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2102 CCAAAAAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1502 CCCGGG C^CCGGG
XmaI 1502 CCCGGG C^CCGGG
XmnI 1011 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 668 AAATTT [GA]^AATT[CT]
1655 AAATTT
AfaI 124 GTAC GT^AC
179 GTAC
AlwI 213 GGATC GGATC(4/5)
1635 GGATC
AlwNI 1474 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2144 CAGCAGCTG
ApoI 668 AAATTT [GA]^AATT[CT]
1655 AAATTT
BalI 558 TGGCCA TGG^CCA
1169 TGGCCA
BmyI 235 GTGCAC G[AGT]GC[ACT]^C
1718 GAGCCC
BpmI 1734 CTCCAG CTGGAG(16/14)
271 CTGGAG
BsgI 1483 CTGCAC GTGCAG(16/14)
1264 GTGCAG
BsmAI 2138 GAGAC GTCTC(1/5)
1732 GTCTC
BsmI 1353 GAATGC GAATGC(1/-1)
726 GCATTC
Bsp1286I 235 GTGCAC G[AGT]GC[ACT]^C
1718 GAGCCC
BsrI 737 ACTGG ACTGG(1/-1)
1736 CCAGT
BstXI 1379 CCATGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1671 CCATGAAGATGG
EaeI 558 TGGCCA [CT]^GGCC[GA]
1169 TGGCCA
HapII 1503 CCGG C^CGG
1926 CCGG
HgaI 704 GCGTC GACGC(5/10)
1730 GCGTC
HpaII 1503 CCGG C^CGG
1926 CCGG
MluNI 558 TGGCCA TGG^CCA
1169 TGGCCA
MscI 558 TGGCCA TGG^CCA
1169 TGGCCA
MspI 1503 CCGG C^CGG
1926 CCGG
PleI 1375 GACTC GAGTC(4/5)
687 GAGTC
RsaI 124 GTAC GT^AC
179 GTAC
SapI 1899 GAAGAGC GCTCTTC(1/4)
1951 GAAGAGC
SmlI 613 CTTGAG C^T[CT][GA]AG
1208 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI BbsI BbvCI BbvI
BcnI BpuAI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I