Restriction map for
pF1KB3126
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AvaI AviII AvrII BbrPI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FauI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MluI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 2026 GACGTC GACGT^C
AccI 419 GTATAC GT^[AC][GT]AC
AccII 1557 CGCG CG^CG
AccIII 660 TCCGGA T^CCGGA
Alw44I 511 GTGCAC G^TGCAC
AocI 244 CCTCAGG CC^T[ACGT]AGG
ApaLI 511 GTGCAC G^TGCAC
AseI 961 ATTAAT AT^TAAT
AsnI 961 ATTAAT AT^TAAT
Asp700I 85 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2333 TGGCCA TGG^CCA
BamHI 51 GGATCC G^GATCC
BbeI 686 GGCGCC GGCGC^C
BciVI 2525 GGATAC GTATCC(6/5)
BclI 1769 TGATCA T^GATCA
BcnI 2051 CCGGG CC^[GC]GG
BglI 2014 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2589 GGTCTC GGTCTC(1/5)
BsaWI 660 TCCGGA [AT]^CCGG[AT]
BseAI 660 TCCGGA T^CCGGA
BseRI 1178 GAGGAG GAGGAG(10/8)
BsmBI 1568 GAGACG CGTCTC(1/5)
BsmI 1956 GAATGC GAATGC(1/-1)
BspEI 660 TCCGGA T^CCGGA
BsrFI 554 ACCGGC [GA]^CCGG[CT]
Bst1107I 419 GTATAC GTA^TAC
BstUI 1557 CGCG CG^CG
BstZ17I 419 GTATAC GTA^TAC
Bsu36I 244 CCTCAGG CC^T[ACGT]AGG
Cfr10I 554 ACCGGC [GA]^CCGG[CT]
DrdI 391 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 244 CCTCAGG CC^T[ACGT]AGG
EcoT22I 737 ATGCAT ATGCA^T
FbaI 1769 TGATCA T^GATCA
KasI 686 GGCGCC G^GCGCC
MfeI 2651 CAATTG C^AATTG
MluNI 2333 TGGCCA TGG^CCA
MroI 660 TCCGGA T^CCGGA
MscI 2333 TGGCCA TGG^CCA
MunI 2651 CAATTG C^AATTG
MvnI 1557 CGCG CG^CG
NarI 686 GGCGCC GG^CGCC
NciI 2051 CCGGG CC^[GC]GG
NdeI 2375 CATATG CA^TATG
NsiI 737 ATGCAT ATGCA^T
PshBI 961 ATTAAT AT^TAAT
SacI 2749 GAGCTC GAGCT^C
SapI 2206 GCTCTTC GCTCTTC(1/4)
ScaI 2140 AGTACT AGT^ACT
SexAI 207 ACCTGGT A^CC[AT]GGT
SfoI 686 GGCGCC GGC^GCC
SmlI 1903 CTCAAG C^T[CT][GA]AG
SpeI 1021 ACTAGT A^CTAGT
StuI 28 AGGCCT AGG^CCT
XmnI 85 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 800 AAATTT [GA]^AATT[CT]
2471 AAATTC
AlwNI 977 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2766 CAGTGTCTG
ApoI 800 AAATTT [GA]^AATT[CT]
2471 AAATTC
AspI 1630 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2764 GACAGTGTC
BanII 1408 GAGCCC G[GA]GC[CT]^C
2749 GAGCTC
BbsI 2685 GAAGAC GAAGAC(2/6)
934 GTCTTC
BbvCI 624 CCTCAGC CCTCAGC(-5/-2)
1803 GCTGAGG
BpuAI 2685 GAAGAC GAAGAC(2/6)
934 GTCTTC
BspMI 320 ACCTGC ACCTGC(4/8)
2346 GCAGGT
EcoNI 2726 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2887 CCTCTAGAAGG
HaeII 686 GGCGCC [GA]GCGC^[CT]
1595 AGCGCC
HgaI 709 GACGC GACGC(5/10)
1749 GACGC
NcoI 195 CCATGG C^CATGG
1301 CCATGG
PflFI 1630 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2764 GACAGTGTC
PpuMI 1322 AGGACCC [GA]G^G[AT]CC[CT]
2883 AGGACCT
PstI 601 CTGCAG CTGCA^G
2449 CTGCAG
SfcI 601 CTGCAG C^T[GA][CT]AG
2449 CTGCAG
SphI 324 GCATGC GCATG^C
1533 GCATGC
TaqI 846 TCGA T^CGA
1550 TCGA
TfiI 843 GATTC G^A[AT]TC
1312 GAATC
Tth111I 1630 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2764 GACAGTGTC
TthHB8I 846 TCGA T^CGA
1550 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BbvI BfaI
BmyI BpmI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SspI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XbaI
XcmI XhoII