Restriction map for
pF1KB9537
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BanII BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstYI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MaeI MamI MfeI MflI MluI
MroI MseI MunI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 463 GTCTAC GT^[AC][GT]AC
AflIII 809 ACATGT A^C[GA][CT]GT
Alw44I 745 GTGCAC G^TGCAC
AocI 987 CCTGAGG CC^T[ACGT]AGG
ApaLI 745 GTGCAC G^TGCAC
Asp700I 420 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1083 TGCGCA TGC^GCA
BalI 803 TGGCCA TGG^CCA
BbeI 43 GGCGCC GGCGC^C
BbvCI 1003 GCTGAGG CCTCAGC(-5/-2)
BglI 879 GCCCGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 369 TCCGGT [AT]^CCGG[AT]
BspHI 908 TCATGA T^CATGA
BspLU11I 809 ACATGT A^CATGT
BsrBI 513 CCGCTC CCGCTC(-3/-3)
BsrFI 134 GCCGGC [GA]^CCGG[CT]
BstBI 1027 TTCGAA TT^CGAA
BstXI 666 CCAGCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 987 CCTGAGG CC^T[ACGT]AGG
Cfr10I 134 GCCGGC [GA]^CCGG[CT]
DpnII 690 GATC ^GATC
DraIII 739 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 803 TGGCCA [CT]^GGCC[GA]
Eco81I 987 CCTGAGG CC^T[ACGT]AGG
FspI 1083 TGCGCA TGC^GCA
HgaI 150 GACGC GACGC(5/10)
KasI 43 GGCGCC G^GCGCC
KspI 155 CCGCGG CCGC^GG
MboI 690 GATC ^GATC
MluNI 803 TGGCCA TGG^CCA
MscI 803 TGGCCA TGG^CCA
MslI 1059 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 134 GCCGGC GCC^GGC
NarI 43 GGCGCC GG^CGCC
NdeII 690 GATC ^GATC
NgoMIV 134 GCCGGC G^CCGGC
NspI 809 ACATGT [GA]CATG^[CT]
NspV 1027 TTCGAA TT^CGAA
PpuMI 913 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1144 CTGCAG CTGCA^G
RcaI 908 TCATGA T^CATGA
SacII 155 CCGCGG CCGC^GG
Sau3AI 690 GATC ^GATC
SexAI 740 ACCAGGT A^CC[AT]GGT
SfcI 1144 CTGCAG C^T[GA][CT]AG
SfoI 43 GGCGCC GGC^GCC
SfuI 1027 TTCGAA TT^CGAA
SmaI 12 CCCGGG CCC^GGG
SmlI 955 CTCAAG C^T[CT][GA]AG
XmaCI 12 CCCGGG C^CCGGG
XmaI 12 CCCGGG C^CCGGG
XmnI 420 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcyI 43 GGCGCC G[GA]^CG[CT]C
150 GACGCC
AspHI 256 GAGCAC G[AT]GC[AT]^C
745 GTGCAC
AvaI 12 CCCGGG C^[CT]CG[GA]G
921 CTCGGG
BsaHI 43 GGCGCC G[GA]^CG[CT]C
150 GACGCC
BsiHKAI 256 GAGCAC G[AT]GC[AT]^C
745 GTGCAC
BsmBI 30 CGTCTC CGTCTC(1/5)
197 GAGACG
BsoBI 12 CCCGGG C^[CT]CG[GA]G
921 CTCGGG
EcoNI 317 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
987 CCTGAGGGAGG
EcoT14I 683 CCTTGG C^C[AT][AT]GG
1076 CCTTGG
Hin1I 43 GGCGCC G[GA]^CG[CT]C
150 GACGCC
HinfI 264 GACTC G^A[ACGT]TC
871 GACTC
MaeIII 148 GTGAC ^GT[ACGT]AC
622 GTGAC
PleI 264 GACTC GAGTC(4/5)
871 GACTC
StyI 683 CCTTGG C^C[AT][AT]GG
1076 CCTTGG
Tsp45I 148 GTGAC ^GT[GC]AC
622 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BanI BbvI BcnI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PflMI PvuII RsaI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I Van91I