Restriction map for
pF1KB8182
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT14I
FbaI FseI FspI HaeII HgaI
Hin1I HindIII KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 349 CGCG CG^CG
AcsI 581 AAATTC [GA]^AATT[CT]
AlwNI 167 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 581 AAATTC [GA]^AATT[CT]
AspHI 468 GAGCTC G[AT]GC[AT]^C
BalI 635 TGGCCA TGG^CCA
BanI 160 GGTGCC G^G[CT][GA]CC
BanII 468 GAGCTC G[GA]GC[CT]^C
BbsI 376 GAAGAC GAAGAC(2/6)
BbvCI 199 GCTGAGG CCTCAGC(-5/-2)
BcnI 24 CCGGG CC^[GC]GG
BmyI 468 GAGCTC G[AGT]GC[ACT]^C
BpmI 457 CTGGAG CTGGAG(16/14)
BpuAI 376 GAAGAC GAAGAC(2/6)
BsaJI 62 CCGTGG C^C[ACGT][ACGT]GG
BsiHKAI 468 GAGCTC G[AT]GC[AT]^C
BsmFI 7 GGGAC GGGAC(10/14)
BsmI 430 GCATTC GAATGC(1/-1)
Bsp1286I 468 GAGCTC G[AGT]GC[ACT]^C
BsrBI 629 CCGCTC CCGCTC(-3/-3)
BstBI 129 TTCGAA TT^CGAA
BstEII 27 GGTCACC G^GT[ACGT]ACC
BstNI 366 CCTGG CC^[AT]GG
BstPI 27 GGTCACC G^GT[ACGT]ACC
BstUI 349 CGCG CG^CG
BstXI 263 CCAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 350 GCGC GCG^C
DsaI 62 CCGTGG C^C[GA][CT]GG
EaeI 635 TGGCCA [CT]^GGCC[GA]
EcoO65I 27 GGTCACC G^GT[ACGT]ACC
EcoRII 366 CCTGG ^CC[AT]GG
EcoT22I 428 ATGCAT ATGCA^T
FauI 20 CCCGC CCCGC(4/6)
HhaI 350 GCGC GCG^C
HinP1I 350 GCGC G^CGC
HincII 303 GTTAAC GT[CT]^[GA]AC
HindII 303 GTTAAC GT[CT]^[GA]AC
HpaI 303 GTTAAC GTT^AAC
MluNI 635 TGGCCA TGG^CCA
MscI 635 TGGCCA TGG^CCA
MseI 304 TTAA T^TAA
MvaI 366 CCTGG CC^[AT]GG
MvnI 349 CGCG CG^CG
NciI 24 CCGGG CC^[GC]GG
NsiI 428 ATGCAT ATGCA^T
NspI 677 GCATGC [GA]CATG^[CT]
NspV 129 TTCGAA TT^CGAA
PstI 571 CTGCAG CTGCA^G
SacI 468 GAGCTC GAGCT^C
SfuI 129 TTCGAA TT^CGAA
SmlI 682 CTTGAG C^T[CT][GA]AG
SphI 677 GCATGC GCATG^C
Tru9I 304 TTAA T^TAA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 8 GGACC G^G[AT]CC
273 GGACC
BfaI 386 CTAG C^TAG
452 CTAG
BsmAI 135 GTCTC GTCTC(1/5)
549 GTCTC
BssKI 24 CCGGG ^CC[ACGT]GG
366 CCTGG
Cfr13I 8 GGACC G^G[ACGT]CC
273 GGACC
HaeIII 576 GGCC GG^CC
636 GGCC
HapII 24 CCGG C^CGG
78 CCGG
HpaII 24 CCGG C^CGG
78 CCGG
MaeI 386 CTAG C^TAG
452 CTAG
MslI 57 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
60 CACCGTGGTG
MspI 24 CCGG C^CGG
78 CCGG
MwoI 154 GCTTATGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
414 GCTAAAGAAGC
NlaIII 664 CATG CATG^
678 CATG
NlaIV 7 GGGACC GG[ACGT]^[ACGT]CC
160 GGTGCC
PleI 485 GAGTC GAGTC(4/5)
547 GAGTC
PvuII 391 CAGCTG CAG^CTG
445 CAGCTG
Sau96I 8 GGACC G^G[ACGT]CC
273 GGACC
ScrFI 24 CCGGG CC^[ACGT]GG
366 CCTGG
SfaNI 427 GATGC GCATC(5/9)
74 GCATC
SfcI 480 CTACAG C^T[GA][CT]AG
571 CTGCAG
TfiI 112 GAATC G^A[AT]TC
232 GAATC
Tsp45I 28 GTCAC ^GT[GC]AC
487 GTCAC
Tsp509I 182 AATT ^AATT
582 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BbvI BseRI BsiYI
BslI BsrI BstF5I Cac8I DdeI
DpnII EarI Fnu4HI FokI HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I NdeII Sau3AI
TaqI TseI TspRI TthHB8I