Restriction map for
pF1KA1150
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BspMI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 711 AAATTT [GA]^AATT[CT]
ApoI 711 AAATTT [GA]^AATT[CT]
AspI 560 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 594 GAAGAC GAAGAC(2/6)
BbvCI 504 GCTGAGG CCTCAGC(-5/-2)
BcnI 1745 CCCGG CC^[GC]GG
BglI 1054 GCCAAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 594 GAAGAC GAAGAC(2/6)
BsaI 1498 GAGACC GGTCTC(1/5)
BseRI 478 GAGGAG GAGGAG(10/8)
BsgI 1145 GTGCAG GTGCAG(16/14)
BsmI 612 GAATGC GAATGC(1/-1)
Bsp1407I 606 TGTACA T^GTACA
BspHI 1505 TCATGA T^CATGA
BspLU11I 773 ACATGT A^CATGT
BsrFI 1376 ACCGGC [GA]^CCGG[CT]
BsrGI 606 TGTACA T^GTACA
BstYI 1097 AGATCC [GA]^GATC[CT]
CfoI 1019 GCGC GCG^C
Cfr10I 1376 ACCGGC [GA]^CCGG[CT]
DsaI 116 CCATGG C^C[GA][CT]GG
EcoNI 1404 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1388 ATGCAT ATGCA^T
HaeII 1018 AGCGCC [GA]GCGC^[CT]
HhaI 1019 GCGC GCG^C
HinP1I 1019 GCGC G^CGC
HincII 1651 GTCAAC GT[CT]^[GA]AC
HindII 1651 GTCAAC GT[CT]^[GA]AC
HindIII 670 AAGCTT A^AGCTT
MflI 1097 AGATCC [GA]^GATC[CT]
NciI 1745 CCCGG CC^[GC]GG
NcoI 116 CCATGG C^CATGG
NsiI 1388 ATGCAT ATGCA^T
NspI 773 ACATGT [GA]CATG^[CT]
PflFI 560 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1049 CTGCAG CTGCA^G
RcaI 1505 TCATGA T^CATGA
SmlI 169 CTTGAG C^T[CT][GA]AG
SspBI 606 TGTACA T^GTACA
StuI 250 AGGCCT AGG^CCT
Tsp45I 731 GTCAC ^GT[GC]AC
Tth111I 560 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 413 CCAGTTCCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1097 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflIII 773 ACATGT A^C[GA][CT]GT
789 ACGTGT
AlwI 1098 GATCC GGATC(4/5)
1737 GATCC
ApaI 689 GGGCCC GGGCC^C
831 GGGCCC
BanI 174 GGTGCC G^G[CT][GA]CC
1605 GGCACC
BanII 689 GGGCCC G[GA]GC[CT]^C
831 GGGCCC
BsrBI 1578 CCGCTC CCGCTC(-3/-3)
481 GAGCGG
BsrDI 140 CATTGC GCAATG(2/0)
1656 CATTGC
DpnII 1098 GATC ^GATC
1737 GATC
EarI 23 CTCTTC CTCTTC(1/4)
1064 CTCTTC
HapII 1377 CCGG C^CGG
1746 CCGG
HpaII 1377 CCGG C^CGG
1746 CCGG
Ksp632I 23 CTCTTC CTCTTC(1/4)
1064 CTCTTC
MaeII 123 ACGT A^CGT
789 ACGT
MaeIII 186 GTTAC ^GT[ACGT]AC
731 GTCAC
MboI 1098 GATC ^GATC
1737 GATC
MspI 1377 CCGG C^CGG
1746 CCGG
NdeII 1098 GATC ^GATC
1737 GATC
SapI 22 GCTCTTC GCTCTTC(1/4)
1063 GCTCTTC
Sau3AI 1098 GATC ^GATC
1737 GATC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NlaIII NlaIV PleI PvuII RsaI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI