Restriction map for
pF1KE1054
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
Acc65I AccI AccIII AclI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BseRI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
Ksp632I KspI MamI MluI MluNI
MroI MscI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1261 GACGTC GACGT^C
AccII 38 CGCG CG^CG
AcyI 1261 GACGTC G[GA]^CG[CT]C
AflIII 1002 ACATGT A^C[GA][CT]GT
AgeI 1276 ACCGGT A^CCGGT
AlwNI 1493 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 939 AGCGCT AGC^GCT
Asp700I 396 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 25 GGACC G^G[AT]CC
BanII 741 GGGCTC G[GA]GC[CT]^C
BfaI 392 CTAG C^TAG
BglII 788 AGATCT A^GATCT
BmyI 741 GGGCTC G[AGT]GC[ACT]^C
BsaHI 1261 GACGTC G[GA]^CG[CT]C
BsgI 132 GTGCAG GTGCAG(16/14)
BsiEI 1278 CGGTCG CG[GA][CT]^CG
BsmI 905 GCATTC GAATGC(1/-1)
Bsp1286I 741 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 1002 ACATGT A^CATGT
BsrDI 1415 GCAATG GCAATG(2/0)
BsrFI 1276 ACCGGT [GA]^CCGG[CT]
BstEII 697 GGTAACC G^GT[ACGT]ACC
BstPI 697 GGTAACC G^GT[ACGT]ACC
BstUI 38 CGCG CG^CG
Cfr10I 1276 ACCGGT [GA]^CCGG[CT]
DrdI 381 GACGAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 602 TGGCCG [CT]^GGCC[GA]
Eco47III 939 AGCGCT AGC^GCT
EcoNI 8 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 697 GGTAACC G^GT[ACGT]ACC
EcoT14I 32 CCATGG C^C[AT][AT]GG
FauI 28 CCCGC CCCGC(4/6)
HaeII 939 AGCGCT [GA]GCGC^[CT]
HgaI 1318 GCGTC GACGC(5/10)
Hin1I 1261 GACGTC G[GA]^CG[CT]C
HincII 835 GTCAAC GT[CT]^[GA]AC
HindII 835 GTCAAC GT[CT]^[GA]AC
HindIII 263 AAGCTT A^AGCTT
MaeI 392 CTAG C^TAG
MspA1I 1039 CAGCTG C[AC]G^C[GT]G
MvnI 38 CGCG CG^CG
NcoI 32 CCATGG C^CATGG
NdeI 715 CATATG CA^TATG
PinAI 1276 ACCGGT A^CCGGT
PvuII 1039 CAGCTG CAG^CTG
ScaI 1544 AGTACT AGT^ACT
SexAI 701 ACCTGGT A^CC[AT]GGT
StyI 32 CCATGG C^C[AT][AT]GG
TaqI 875 TCGA T^CGA
TthHB8I 875 TCGA T^CGA
XcmI 937 CCAGCGCTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 396 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 209 AAATTT [GA]^AATT[CT]
310 AAATTT
AflII 747 CTTAAG C^TTAAG
1360 CTTAAG
ApoI 209 AAATTT [GA]^AATT[CT]
310 AAATTT
BfrI 747 CTTAAG C^TTAAG
1360 CTTAAG
BpmI 1036 CTCCAG CTGGAG(16/14)
75 CTGGAG
BsaWI 669 TCCGGT [AT]^CCGG[AT]
1276 ACCGGT
BstXI 1542 CCAGTACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1576 CCAGACACATGG
CfoI 37 GCGC GCG^C
940 GCGC
DsaI 32 CCATGG C^C[GA][CT]GG
1026 CCACGG
HapII 670 CCGG C^CGG
1277 CCGG
HhaI 37 GCGC GCG^C
940 GCGC
HinP1I 37 GCGC G^CGC
940 GCGC
HpaII 670 CCGG C^CGG
1277 CCGG
MaeII 100 ACGT A^CGT
1262 ACGT
MfeI 290 CAATTG C^AATTG
576 CAATTG
MspI 670 CCGG C^CGG
1277 CCGG
MunI 290 CAATTG C^AATTG
576 CAATTG
NspI 451 ACATGC [GA]CATG^[CT]
1002 ACATGT
SspI 547 AATATT AAT^ATT
966 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BsaJI BsiYI BslI BsmFI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII