##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwI        AlwNI       AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DpnII       
DraII       DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       ItaI        KasI        KpnI        
KspI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        SwaI        TaqI        TseI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AfaI          563  GTAC                 GT^AC
Alw44I        587  GTGCAC               G^TGCAC
ApaLI         587  GTGCAC               G^TGCAC
AspHI         587  GTGCAC               G[AT]GC[AT]^C
AvaII         446  GGTCC                G^G[AT]CC
BmyI          587  GTGCAC               G[AGT]GC[ACT]^C
BsaJI         189  CCAAGG               C^C[ACGT][ACGT]GG
BsiHKAI       587  GTGCAC               G[AT]GC[AT]^C
BsiYI         437  CCTTTAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          437  CCTTTAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI           6  GAGAC                GTCTC(1/5)
Bsp1286I      587  GTGCAC               G[AGT]GC[ACT]^C
BstF5I        171  GGATG                GGATG(2/0)
Cac8I         359  GCATGC               GC[ACGT]^[ACGT]GC
Cfr13I        446  GGTCC                G^G[ACGT]CC
DraI          607  TTTAAA               TTT^AAA
DraIII        590  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I       189  CCAAGG               C^C[AT][AT]GG
EcoT22I       271  ATGCAT               ATGCA^T
FokI          171  GGATG                GGATG(9/13)
HindIII       770  AAGCTT               A^AGCTT
HinfI          88  GATTC                G^A[ACGT]TC
HphI          525  TCACC                GGTGA(8/7)
MslI          746  CATACAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV         741  GGAACC               GG[ACGT]^[ACGT]CC
NsiI          271  ATGCAT               ATGCA^T
NspI          359  GCATGC               [GA]CATG^[CT]
RsaI          563  GTAC                 GT^AC
Sau96I        446  GGTCC                G^G[ACGT]CC
SphI          359  GCATGC               GCATG^C
SspI          240  AATATT               AAT^ATT
StyI          189  CCAAGG               C^C[AT][AT]GG
TfiI           88  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AluI           36  AGCT                 AG^CT
              771  AGCT                
BsmFI         183  GGGAC                GGGAC(10/14)
              699  GTCCC               
DdeI          109  CTGAG                C^T[ACGT]AG
              467  CTAAG               
MaeIII        103  GTGAC                ^GT[ACGT]AC
              344  GTGAC               
Tsp45I        103  GTGAC                ^GT[GC]AC
              344  GTGAC               
TspRI         107  CACTG                CA[GC]TG[ACGT][ACGT]^
              592  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AcsI        ApoI        BfaI        BpmI        BsrI        
EarI        Ksp632I     MaeI        MboII       MnlI        
MseI        NlaIII      Tru9I       Tsp509I