Restriction map for
pF1KB8986
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII AccI AccII AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI SacII SalI
SbfI ScaI SexAI SfcI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 476 GGTACC G^GTACC
AflII 862 CTTAAG C^TTAAG
AlwNI 742 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 476 GGTACC G^GTACC
AvaI 634 CCCGAG C^[CT]CG[GA]G
AvrII 546 CCTAGG C^CTAGG
BanI 476 GGTACC G^G[CT][GA]CC
BanII 51 GAGCTC G[GA]GC[CT]^C
BbvCI 148 GCTGAGG CCTCAGC(-5/-2)
BfaI 547 CTAG C^TAG
BfrI 862 CTTAAG C^TTAAG
BglI 79 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 546 CCTAGG C^CTAGG
BsaWI 515 ACCGGA [AT]^CCGG[AT]
BseRI 1068 CTCCTC GAGGAG(10/8)
BsgI 523 GTGCAG GTGCAG(16/14)
BsmAI 856 GAGAC GTCTC(1/5)
BsmBI 856 GAGACG CGTCTC(1/5)
BsmI 616 GAATGC GAATGC(1/-1)
BsoBI 634 CCCGAG C^[CT]CG[GA]G
BssSI 1131 CACGAG CACGAG(-5/-1)
BstF5I 738 CATCC GGATG(2/0)
BstYI 903 GGATCT [GA]^GATC[CT]
CfoI 1047 GCGC GCG^C
CpoI 517 CGGACCG CG^G[AT]CCG
DrdI 169 GACATCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 95 CCATGG C^C[GA][CT]GG
FokI 738 CATCC GGATG(9/13)
HaeII 1046 GGCGCT [GA]GCGC^[CT]
HapII 516 CCGG C^CGG
HhaI 1047 GCGC GCG^C
HinP1I 1047 GCGC G^CGC
HpaII 516 CCGG C^CGG
KpnI 476 GGTACC GGTAC^C
MaeI 547 CTAG C^TAG
MaeII 1058 ACGT A^CGT
MflI 903 GGATCT [GA]^GATC[CT]
MspI 516 CCGG C^CGG
NcoI 95 CCATGG C^CATGG
PleI 357 GACTC GAGTC(4/5)
PpuMI 638 AGGTCCT [GA]G^G[AT]CC[CT]
RsrII 517 CGGACCG CG^G[AT]CCG
SacI 51 GAGCTC GAGCT^C
SapI 48 GAAGAGC GCTCTTC(1/4)
SfaNI 1144 GATGC GCATC(5/9)
SfiI 78 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 862 CTTAAG C^T[CT][GA]AG
StuI 152 AGGCCT AGG^CCT
XhoII 903 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 532 GGCGTC G[GA]^CG[CT]C
550 GGCGTC
AfaI 135 GTAC GT^AC
477 GTAC
AluI 52 AGCT AG^CT
866 AGCT
AspHI 51 GAGCTC G[AT]GC[AT]^C
661 GTGCTC
AvaII 518 GGACC G^G[AT]CC
639 GGTCC
BalI 77 TGGCCA TGG^CCA
482 TGGCCA
BmyI 51 GAGCTC G[AGT]GC[ACT]^C
661 GTGCTC
BsaHI 532 GGCGTC G[GA]^CG[CT]C
550 GGCGTC
BsiHKAI 51 GAGCTC G[AT]GC[AT]^C
661 GTGCTC
BsmFI 167 GGGAC GGGAC(10/14)
184 GGGAC
Bsp1286I 51 GAGCTC G[AGT]GC[ACT]^C
661 GTGCTC
BsrDI 1148 CATTGC GCAATG(2/0)
668 GCAATG
Cac8I 912 GCTGGC GC[ACGT]^[ACGT]GC
1091 GCAGGC
EarI 820 CTCTTC CTCTTC(1/4)
48 GAAGAG
EcoT22I 974 ATGCAT ATGCA^T
1145 ATGCAT
Hin1I 532 GGCGTC G[GA]^CG[CT]C
550 GGCGTC
Ksp632I 820 CTCTTC CTCTTC(1/4)
48 GAAGAG
MluNI 77 TGGCCA TGG^CCA
482 TGGCCA
MscI 77 TGGCCA TGG^CCA
482 TGGCCA
MseI 785 TTAA T^TAA
863 TTAA
MwoI 79 GCCAGTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
947 GCCATTGTTGC
NlaIV 85 GGGGCC GG[ACGT]^[ACGT]CC
476 GGTACC
NsiI 974 ATGCAT ATGCA^T
1145 ATGCAT
RsaI 135 GTAC GT^AC
477 GTAC
Tru9I 785 TTAA T^TAA
863 TTAA
XcmI 224 CCATTGGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1128 CCACACGAGTGATGG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AlwI ApoI BbvI
BpmI BsaJI BsiYI BslI BsrI
BssKI BstNI BstXI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HgaI HinfI
HphI ItaI MaeIII MboI MboII
MnlI MslI MvaI NdeII NlaIII
Sau3AI Sau96I ScrFI StyI TfiI
TseI Tsp45I Tsp509I TspRI