Restriction map for
pF1KB8963
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglII BpuAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI Ksp632I MamI MfeI
MluI MroI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 78 GGCGTC G[GA]^CG[CT]C
Alw44I 528 GTGCAC G^TGCAC
ApaLI 528 GTGCAC G^TGCAC
AvaI 513 CCCGAG C^[CT]CG[GA]G
AvrII 808 CCTAGG C^CTAGG
BanI 804 GGTGCC G^G[CT][GA]CC
BanII 752 GGGCTC G[GA]GC[CT]^C
BclI 599 TGATCA T^GATCA
BfaI 809 CTAG C^TAG
BglI 66 GCCCTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 808 CCTAGG C^CTAGG
BlpI 203 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 203 GCTTAGC GC^T[ACGT]AGC
BsaAI 439 TACGTG [CT]AC^GT[GA]
BsaHI 78 GGCGTC G[GA]^CG[CT]C
BsiEI 470 CGGCCG CG[GA][CT]^CG
BsmBI 1004 CGTCTC CGTCTC(1/5)
BsoBI 513 CCCGAG C^[CT]CG[GA]G
BsrFI 355 ACCGGC [GA]^CCGG[CT]
CelII 203 GCTTAGC GC^T[ACGT]AGC
Cfr10I 355 ACCGGC [GA]^CCGG[CT]
EagI 470 CGGCCG C^GGCCG
EclXI 470 CGGCCG C^GGCCG
Eco52I 470 CGGCCG C^GGCCG
EcoT14I 808 CCTAGG C^C[AT][AT]GG
FbaI 599 TGATCA T^GATCA
HapII 356 CCGG C^CGG
Hin1I 78 GGCGTC G[GA]^CG[CT]C
HpaII 356 CCGG C^CGG
KspI 48 CCGCGG CCGC^GG
MaeI 809 CTAG C^TAG
MspI 356 CCGG C^CGG
NdeI 450 CATATG CA^TATG
PflMI 743 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 861 CAGCTG CAG^CTG
SacII 48 CCGCGG CCGC^GG
SexAI 218 ACCTGGT A^CC[AT]GGT
SfaNI 359 GCATC GCATC(5/9)
SmlI 397 CTCAAG C^T[CT][GA]AG
StyI 808 CCTAGG C^C[AT][AT]GG
TaqI 259 TCGA T^CGA
Tsp45I 712 GTCAC ^GT[GC]AC
TthHB8I 259 TCGA T^CGA
Van91I 743 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 705 GAATTT [GA]^AATT[CT]
900 AAATTT
ApoI 705 GAATTT [GA]^AATT[CT]
900 AAATTT
BalI 227 TGGCCA TGG^CCA
833 TGGCCA
BamHI 632 GGATCC G^GATCC
812 GGATCC
BpmI 502 CTCCAG CTGGAG(16/14)
643 CTCCAG
BseRI 445 CTCCTC GAGGAG(10/8)
755 CTCCTC
BsgI 292 CTGCAC GTGCAG(16/14)
491 GTGCAG
BsmFI 53 GGGAC GGGAC(10/14)
592 GTCCC
Bsp1407I 413 TGTACA T^GTACA
940 TGTACA
BsrGI 413 TGTACA T^GTACA
940 TGTACA
BssHII 17 GCGCGC G^CGCGC
59 GCGCGC
BssSI 69 CTCGTG CACGAG(-5/-1)
525 CTCGTG
BstF5I 123 CATCC GGATG(2/0)
144 CATCC
Cfr13I 65 GGCCC G^G[ACGT]CC
321 GGGCC
FauI 793 CCCGC CCCGC(4/6)
50 GCGGG
FokI 123 CATCC GGATG(9/13)
144 CATCC
HinfI 498 GACTC G^A[ACGT]TC
1042 GACTC
MaeII 440 ACGT A^CGT
977 ACGT
MboII 104 TCTTC GAAGA(8/7)
611 TCTTC
MluNI 227 TGGCCA TGG^CCA
833 TGGCCA
MscI 227 TGGCCA TGG^CCA
833 TGGCCA
MseI 266 TTAA T^TAA
992 TTAA
MspA1I 48 CCGCGG C[AC]G^C[GT]G
861 CAGCTG
PleI 498 GACTC GAGTC(4/5)
1042 GACTC
Sau96I 65 GGCCC G^G[ACGT]CC
321 GGGCC
SspBI 413 TGTACA T^GTACA
940 TGTACA
Tru9I 266 TTAA T^TAA
992 TTAA
XcmI 563 CCATCGCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
836 CCAGTTACTACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BbvI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI BstUI BstXI BstYI
Cac8I CfoI DdeI DpnII DsaI
EaeI EcoRII Fnu4HI HaeIII HgaI
HhaI HinP1I HphI ItaI MaeIII
MboI MflI MnlI MslI MvaI
MvnI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI TseI Tsp509I
TspRI XhoII