Restriction map for
pF1KA2033
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# NO CUT ENZYMES ( 152 enzymes) #
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Acc65I AccII AccIII AciI AclI
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT22I FauI FseI
FspI HaeII HgaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspA1I MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflII 687 CTTAAG C^TTAAG
AlwI 421 GGATC GGATC(4/5)
AvaII 75 GGACC G^G[AT]CC
BbsI 1300 GAAGAC GAAGAC(2/6)
BbvI 1580 GCAGC GCAGC(8/12)
BclI 378 TGATCA T^GATCA
BfrI 687 CTTAAG C^TTAAG
BpuAI 1300 GAAGAC GAAGAC(2/6)
BsaI 132 GGTCTC GGTCTC(1/5)
BsaWI 1885 ACCGGA [AT]^CCGG[AT]
BseRI 58 GAGGAG GAGGAG(10/8)
BsgI 1578 GTGCAG GTGCAG(16/14)
BsmAI 133 GTCTC GTCTC(1/5)
BspHI 282 TCATGA T^CATGA
BspMI 1612 ACCTGC ACCTGC(4/8)
BsrDI 218 CATTGC GCAATG(2/0)
CfoI 184 GCGC GCG^C
DsaI 573 CCATGG C^C[GA][CT]GG
EcoRV 142 GATATC GAT^ATC
FbaI 378 TGATCA T^GATCA
Fnu4HI 1580 GCAGC GC^[ACGT]GC
HhaI 184 GCGC GCG^C
HinP1I 184 GCGC G^CGC
HpaI 1686 GTTAAC GTT^AAC
HphI 796 GGTGA GGTGA(8/7)
ItaI 1580 GCAGC GC^[ACGT]GC
MwoI 444 GCCTGAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 573 CCATGG C^CATGG
PleI 90 GACTC GAGTC(4/5)
RcaI 282 TCATGA T^CATGA
SexAI 128 ACCTGGT A^CC[AT]GGT
SmlI 687 CTTAAG C^T[CT][GA]AG
SphI 772 GCATGC GCATG^C
TaqI 1035 TCGA T^CGA
TseI 1580 GCAGC G^C[AT]GC
TspRI 792 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 1035 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 365 GTAC GT^AC
622 GTAC
Asp700I 165 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
579 GAATAATTTC
BalI 38 TGGCCA TGG^CCA
321 TGGCCA
BfaI 392 CTAG C^TAG
769 CTAG
BsmFI 101 GGGAC GGGAC(10/14)
214 GGGAC
Cac8I 743 GCAAGC GC[ACGT]^[ACGT]GC
772 GCATGC
Cfr13I 75 GGACC G^G[ACGT]CC
548 GGCCC
EaeI 38 TGGCCA [CT]^GGCC[GA]
321 TGGCCA
EcoT14I 515 CCTTGG C^C[AT][AT]GG
573 CCATGG
HapII 1344 CCGG C^CGG
1886 CCGG
HpaII 1344 CCGG C^CGG
1886 CCGG
MaeI 392 CTAG C^TAG
769 CTAG
MluNI 38 TGGCCA TGG^CCA
321 TGGCCA
MscI 38 TGGCCA TGG^CCA
321 TGGCCA
MspI 1344 CCGG C^CGG
1886 CCGG
NlaIV 75 GGACCC GG[ACGT]^[ACGT]CC
125 GGAACC
RsaI 365 GTAC GT^AC
622 GTAC
Sau96I 75 GGACC G^G[ACGT]CC
548 GGCCC
StyI 515 CCTTGG C^C[AT][AT]GG
573 CCATGG
TfiI 801 GAATC G^A[AT]TC
1809 GAATC
XmnI 165 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
579 GAATAATTTC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AatII AccI AcsI AcyI AhdI
AluI ApoI AspEI BpmI BsaHI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI DdeI DpnII Eam1105I
EarI EcoRI EcoRII FokI HaeIII
Hin1I HincII HindII HindIII HinfI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII NspI Sau3AI ScrFI SfaNI
Tru9I Tsp45I Tsp509I