Restriction map for
pF1KB0999
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbrPI
BciVI BfrI BlnI BsaBI BseAI
BspEI Bst1107I BstBI BstZ17I CpoI
DraI Eam1105I EcoRI EcoRV EcoT22I
FspI HpaI KpnI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NruI NsiI NspV PacI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SfuI SnaBI SpeI
SspI SwaI Tru9I Tsp509I XbaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AflIII 1289 ACATGT A^C[GA][CT]GT
AgeI 438 ACCGGT A^CCGGT
Aor51HI 899 AGCGCT AGC^GCT
Asp700I 1002 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1932 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BlpI 6486 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 6486 GCTCAGC GC^T[ACGT]AGC
BsaAI 4111 TACGTG [CT]AC^GT[GA]
BsiWI 4440 CGTACG C^GTACG
BspDI 1126 ATCGAT AT^CGAT
BspLU11I 1289 ACATGT A^CATGT
BsrDI 4587 CATTGC GCAATG(2/0)
BssHII 4591 GCGCGC G^CGCGC
CelII 6486 GCTCAGC GC^T[ACGT]AGC
ClaI 1126 ATCGAT AT^CGAT
DrdI 1273 GACCGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 899 AGCGCT AGC^GCT
FseI 2618 GGCCGGCC GGCCGG^CC
HindIII 6307 AAGCTT A^AGCTT
PaeR7I 3376 CTCGAG C^TCGAG
PflFI 1932 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 438 ACCGGT A^CCGGT
PshAI 6657 GACGTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 4782 GTCGAC G^TCGAC
SbfI 4728 CCTGCAGG CCTGCA^GG
ScaI 992 AGTACT AGT^ACT
Sse8387I 4728 CCTGCAGG CCTGCA^GG
Tth111I 1932 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3376 CTCGAG C^TCGAG
XmnI 1002 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 4782 GTCGAC GT^[AC][GT]AC
5806 GTCTAC
AocI 1658 CCTCAGG CC^T[ACGT]AGG
5715 CCTGAGG
BamHI 7 GGATCC G^GATCC
4097 GGATCC
BclI 3209 TGATCA T^GATCA
7199 TGATCA
BfaI 328 CTAG C^TAG
5974 CTAG
BsmBI 1449 GAGACG CGTCTC(1/5)
3931 GAGACG
BspHI 798 TCATGA T^CATGA
1283 TCATGA
Bsu36I 1658 CCTCAGG CC^T[ACGT]AGG
5715 CCTGAGG
DraIII 1108 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
6560 CACCCAGTG
Eco81I 1658 CCTCAGG CC^T[ACGT]AGG
5715 CCTGAGG
FbaI 3209 TGATCA T^GATCA
7199 TGATCA
HincII 2224 GTCAAC GT[CT]^[GA]AC
4782 GTCGAC
HindII 2224 GTCAAC GT[CT]^[GA]AC
4782 GTCGAC
MaeI 328 CTAG C^TAG
5974 CTAG
NotI 4353 GCGGCCGC GC^GGCCGC
6342 GCGGCCGC
RcaI 798 TCATGA T^CATGA
1283 TCATGA
SapI 4548 GAAGAGC GCTCTTC(1/4)
6247 GAAGAGC
SexAI 3261 ACCTGGT A^CC[AT]GGT
5508 ACCTGGT
SfiI 2643 GGCCGCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5864 GGCCAGAGCGGCC
TfiI 729 GATTC G^A[AT]TC
861 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BcnI BglI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SgrAI SmaI SmlI SphI
SspBI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI