Restriction map for
pF1KSDA0123
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsmFI
BspDI BspEI BsrBI BsrDI BssHII
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StyI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 354 GTCTAC GT^[AC][GT]AC
AcyI 1544 GGCGCC G[GA]^CG[CT]C
AflIII 908 ACATGT A^C[GA][CT]GT
Alw44I 1395 GTGCAC G^TGCAC
AlwI 289 GGATC GGATC(4/5)
AlwNI 1354 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 723 CCTGAGG CC^T[ACGT]AGG
ApaLI 1395 GTGCAC G^TGCAC
BalI 1361 TGGCCA TGG^CCA
BbeI 1544 GGCGCC GGCGC^C
BbvCI 66 GCTGAGG CCTCAGC(-5/-2)
BcgI 23 CGACGCGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 898 CTAG C^TAG
BglI 493 GCCTTACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 862 AGATCT A^GATCT
BsaAI 1503 CACGTA [CT]AC^GT[GA]
BsaHI 1544 GGCGCC G[GA]^CG[CT]C
BseRI 991 GAGGAG GAGGAG(10/8)
BsiEI 744 CGACCG CG[GA][CT]^CG
BsmI 714 GCATTC GAATGC(1/-1)
Bsp1407I 262 TGTACA T^GTACA
BspLU11I 908 ACATGT A^CATGT
BsrGI 262 TGTACA T^GTACA
BstEII 1483 GGTGACC G^GT[ACGT]ACC
BstPI 1483 GGTGACC G^GT[ACGT]ACC
BstXI 873 CCAGTACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 862 AGATCT [GA]^GATC[CT]
Bsu36I 723 CCTGAGG CC^T[ACGT]AGG
DraII 578 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1521 GACCGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 723 CCTGAGG CC^T[ACGT]AGG
EcoO109I 578 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1483 GGTGACC G^GT[ACGT]ACC
EcoRI 272 GAATTC G^AATTC
FauI 36 GCGGG CCCGC(4/6)
Hin1I 1544 GGCGCC G[GA]^CG[CT]C
KasI 1544 GGCGCC G^GCGCC
MaeI 898 CTAG C^TAG
MflI 862 AGATCT [GA]^GATC[CT]
MluNI 1361 TGGCCA TGG^CCA
MscI 1361 TGGCCA TGG^CCA
MseI 1240 TTAA T^TAA
MspA1I 1288 CAGCTG C[AC]G^C[GT]G
NarI 1544 GGCGCC GG^CGCC
PleI 1436 GAGTC GAGTC(4/5)
PpuMI 578 AGGACCT [GA]G^G[AT]CC[CT]
PstI 514 CTGCAG CTGCA^G
PvuII 1288 CAGCTG CAG^CTG
SacI 946 GAGCTC GAGCT^C
SapI 1566 GCTCTTC GCTCTTC(1/4)
SfoI 1544 GGCGCC GGC^GCC
SspBI 262 TGTACA T^GTACA
StuI 1324 AGGCCT AGG^CCT
Tru9I 1240 TTAA T^TAA
XcmI 914 CCAATGTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 862 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 272 GAATTC [GA]^AATT[CT]
383 AAATTC
ApaI 927 GGGCCC GGGCC^C
1060 GGGCCC
ApoI 272 GAATTC [GA]^AATT[CT]
383 AAATTC
AvaI 42 CTCGGG C^[CT]CG[GA]G
943 CCCGAG
BsmAI 437 GAGAC GTCTC(1/5)
905 GAGAC
BsoBI 42 CTCGGG C^[CT]CG[GA]G
943 CCCGAG
BspHI 627 TCATGA T^CATGA
1304 TCATGA
BssSI 778 CACGAG CACGAG(-5/-1)
1387 CACGAG
DsaI 848 CCGTGG C^C[GA][CT]GG
1253 CCGTGG
EcoNI 719 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
987 CCTGGAGGAGG
HaeII 1183 AGCGCC [GA]GCGC^[CT]
1544 GGCGCC
HphI 1483 GGTGA GGTGA(8/7)
614 TCACC
NspI 908 ACATGT [GA]CATG^[CT]
1073 GCATGT
RcaI 627 TCATGA T^CATGA
1304 TCATGA
SfcI 170 CTACAG C^T[GA][CT]AG
514 CTGCAG
TfiI 624 GATTC G^A[AT]TC
1318 GAATC
Tsp45I 419 GTGAC ^GT[GC]AC
1484 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp509I TspRI TthHB8I