Restriction map for
pF1KE0197
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII Bst1107I
BstAPI BstEII BstF5I BstPI BstYI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DpnII DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HaeIII HgaI Hin1I HincII
HindII HpaI HphI KasI KpnI
KspI MaeI MaeIII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 42 CGCG CG^CG
AciI 41 CCGC CCGC(-3/-1)
AcsI 143 AAATTC [GA]^AATT[CT]
AfaI 243 GTAC GT^AC
ApoI 143 AAATTC [GA]^AATT[CT]
AvaII 27 GGTCC G^G[AT]CC
BbvCI 51 CCTCAGC CCTCAGC(-5/-2)
BbvI 337 GCTGC GCAGC(8/12)
BcnI 182 CCCGG CC^[GC]GG
BmyI 164 GTGCCC G[AGT]GC[ACT]^C
BpmI 247 CTGGAG CTGGAG(16/14)
BsaJI 8 CCAAGG C^C[ACGT][ACGT]GG
BsiYI 341 CCCAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 341 CCCAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 164 GTGCCC G[AGT]GC[ACT]^C
BssSI 31 CTCGTG CACGAG(-5/-1)
BstBI 103 TTCGAA TT^CGAA
BstNI 246 CCTGG CC^[AT]GG
BstUI 42 CGCG CG^CG
BstXI 8 CCAAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 27 GGTCC G^G[ACGT]CC
DraI 258 TTTAAA TTT^AAA
EarI 327 GAAGAG CTCTTC(1/4)
EcoRII 246 CCTGG ^CC[AT]GG
EcoT14I 8 CCAAGG C^C[AT][AT]GG
Fnu4HI 337 GCTGC GC^[ACGT]GC
HapII 183 CCGG C^CGG
HindIII 254 AAGCTT A^AGCTT
HpaII 183 CCGG C^CGG
ItaI 337 GCTGC GC^[ACGT]GC
Ksp632I 327 GAAGAG CTCTTC(1/4)
MaeII 128 ACGT A^CGT
MseI 259 TTAA T^TAA
MspI 183 CCGG C^CGG
MvaI 246 CCTGG CC^[AT]GG
MvnI 42 CGCG CG^CG
NciI 182 CCCGG CC^[GC]GG
NspV 103 TTCGAA TT^CGAA
RsaI 243 GTAC GT^AC
Sau96I 27 GGTCC G^G[ACGT]CC
SfcI 81 CTACAG C^T[GA][CT]AG
SfuI 103 TTCGAA TT^CGAA
StyI 8 CCAAGG C^C[AT][AT]GG
TaqI 104 TCGA T^CGA
Tru9I 259 TTAA T^TAA
TseI 337 GCTGC G^C[AT]GC
Tsp509I 144 AATT ^AATT
TthHB8I 104 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
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BssKI 182 CCCGG ^CC[ACGT]GG
246 CCTGG
CfoI 43 GCGC GCG^C
270 GCGC
DdeI 52 CTCAG C^T[ACGT]AG
76 CTGAG
HhaI 43 GCGC GCG^C
270 GCGC
HinP1I 43 GCGC G^CGC
270 GCGC
HinfI 98 GATTC G^A[ACGT]TC
234 GATTC
MnlI 30 CCTC CCTC(7/6)
51 CCTC
MwoI 36 GCTGACCGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
80 GCTACAGATGC
NlaIII 92 CATG CATG^
112 CATG
ScrFI 182 CCCGG CC^[ACGT]GG
246 CCTGG
SfaNI 86 GATGC GCATC(5/9)
135 GCATC
TfiI 98 GATTC G^A[AT]TC
234 GATTC
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
AluI MboII