Restriction map for
pF1KE0626
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BsaAI BsaHI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 801 GTCTAC GT^[AC][GT]AC
AflIII 144 ACATGT A^C[GA][CT]GT
AlwNI 30 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 559 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 504 CCTAGG C^CTAGG
BclI 684 TGATCA T^GATCA
BcnI 519 CCGGG CC^[GC]GG
BfaI 505 CTAG C^TAG
BglI 815 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 504 CCTAGG C^CTAGG
BmyI 42 GGGCAC G[AGT]GC[ACT]^C
BsaBI 226 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 738 GAGACC GGTCTC(1/5)
BsaWI 793 TCCGGT [AT]^CCGG[AT]
BseRI 121 GAGGAG GAGGAG(10/8)
BsmFI 592 GTCCC GGGAC(10/14)
Bsp1286I 42 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 144 ACATGT A^CATGT
BsrI 530 CCAGT ACTGG(1/-1)
BstAPI 29 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 195 CCATGG C^C[GA][CT]GG
EaeI 251 CGGCCA [CT]^GGCC[GA]
FauI 25 GCGGG CCCGC(4/6)
FbaI 684 TGATCA T^GATCA
HaeIII 252 GGCC GG^CC
HgaI 360 GCGTC GACGC(5/10)
HincII 166 GTTGAC GT[CT]^[GA]AC
HindII 166 GTTGAC GT[CT]^[GA]AC
HphI 54 GGTGA GGTGA(8/7)
MaeI 505 CTAG C^TAG
MamI 226 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 30 CAGCGG C[AC]G^C[GT]G
NciI 519 CCGGG CC^[GC]GG
NcoI 195 CCATGG C^CATGG
PshAI 419 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI 462 GCATC GCATC(5/9)
SfcI 300 CTACAG C^T[GA][CT]AG
XmnI 559 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 156 GATCC GGATC(4/5)
572 GGATC
BlpI 972 GCTAAGC GC^T[ACGT]AGC
1022 GCTTAGC
Bpu1102I 972 GCTAAGC GC^T[ACGT]AGC
1022 GCTTAGC
BsiYI 15 CCGGCACGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
530 CCAGTGTCAGG
BslI 15 CCGGCACGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
530 CCAGTGTCAGG
BsmAI 200 GAGAC GTCTC(1/5)
738 GAGAC
BsrFI 14 ACCGGC [GA]^CCGG[CT]
741 ACCGGC
BstYI 155 AGATCC [GA]^GATC[CT]
572 GGATCT
Cac8I 25 GCGGGC GC[ACGT]^[ACGT]GC
1036 GCAAGC
CelII 972 GCTAAGC GC^T[ACGT]AGC
1022 GCTTAGC
Cfr10I 14 ACCGGC [GA]^CCGG[CT]
741 ACCGGC
EarI 57 GAAGAG CTCTTC(1/4)
675 GAAGAG
EcoT14I 195 CCATGG C^C[AT][AT]GG
504 CCTAGG
HindIII 846 AAGCTT A^AGCTT
975 AAGCTT
Ksp632I 57 GAAGAG CTCTTC(1/4)
675 GAAGAG
MflI 155 AGATCC [GA]^GATC[CT]
572 GGATCT
MslI 396 CATTAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
729 CATAACAGTG
NlaIV 526 GGCTCC GG[ACGT]^[ACGT]CC
564 GGTTCC
NspI 144 ACATGT [GA]CATG^[CT]
1065 ACATGC
PleI 952 GAGTC GAGTC(4/5)
982 GAGTC
SapI 57 GAAGAGC GCTCTTC(1/4)
675 GAAGAGC
StyI 195 CCATGG C^C[AT][AT]GG
504 CCTAGG
TfiI 509 GATTC G^A[AT]TC
918 GATTC
XhoII 155 AGATCC [GA]^GATC[CT]
572 GGATCT
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BpmI BpuAI BsaJI
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HapII HinfI
HpaII ItaI MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
ScrFI Tru9I TseI Tsp509I TspRI