##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BlnI        BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmFI       
BsoBI       BspDI       BspEI       BspMI       BsrBI       
BsrFI       BssHII      BssKI       BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRII      
EcoRV       EcoT14I     FauI        FokI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AflIII       1033  ACATGT               A^C[GA][CT]GT
Alw44I        753  GTGCAC               G^TGCAC
ApaLI         753  GTGCAC               G^TGCAC
AseI          291  ATTAAT               AT^TAAT
AsnI          291  ATTAAT               AT^TAAT
BamHI         136  GGATCC               G^GATCC
BbsI         1159  GAAGAC               GAAGAC(2/6)
BbvCI        1069  GCTGAGG              CCTCAGC(-5/-2)
BclI          251  TGATCA               T^GATCA
BlpI          869  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      869  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1159  GAAGAC               GAAGAC(2/6)
BseRI        1072  GAGGAG               GAGGAG(10/8)
BsmAI        1226  GAGAC                GTCTC(1/5)
BsmI         1254  GAATGC               GAATGC(1/-1)
Bsp1407I     1201  TGTACA               T^GTACA
BspHI        1137  TCATGA               T^CATGA
BspLU11I     1033  ACATGT               A^CATGT
BsrDI         666  CATTGC               GCAATG(2/0)
BsrGI        1201  TGTACA               T^GTACA
CelII         869  GCTCAGC              GC^T[ACGT]AGC
DraIII        756  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         268  CCTTCAGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          251  TGATCA               T^GATCA
NdeI          577  CATATG               CA^TATG
NlaIV         136  GGATCC               GG[ACGT]^[ACGT]CC
PshBI         291  ATTAAT               AT^TAAT
RcaI         1137  TCATGA               T^CATGA
SspBI        1201  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AlwI          137  GATCC                GGATC(4/5)
              136  GGATC               
BbvI          764  GCAGC                GCAGC(8/12)
             1100  GCAGC               
BstAPI        755  GCACACAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              839  GCAGTGAGTGC         
Fnu4HI        764  GCAGC                GC^[ACGT]GC
             1100  GCAGC               
ItaI          764  GCAGC                GC^[ACGT]GC
             1100  GCAGC               
TaqI          131  TCGA                 T^CGA
              359  TCGA                
TseI          764  GCAGC                G^C[AT]GC
             1100  GCAGC               
TthHB8I       131  TCGA                 T^CGA
              359  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        Asp700I     
AspHI       BanII       BglII       BmyI        BpmI        
BsgI        BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrI        BstYI       DdeI        DpnII       EcoRI       
EcoT22I     HindIII     HinfI       HphI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MwoI        NdeII       NlaIII      NsiI        NspI        
RsaI        SacI        Sau3AI      SfaNI       TfiI        
Tru9I       Tsp509I     TspRI       XhoII       XmnI