Restriction map for
pF1KB9626
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpuAI BsaAI BsaHI
BsaI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MaeIII
MboII MfeI MluI MseI MunI
NarI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I TspRI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 231 GTAC GT^AC
AlwNI 181 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 215 GGCGCGCC GG^CGCGCC
BalI 362 TGGCCA TGG^CCA
BamHI 57 GGATCC G^GATCC
BanII 295 GAGCTC G[GA]GC[CT]^C
BcgI 22 GCAGCAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 324 CCCGG CC^[GC]GG
BlpI 186 GCTGAGC GC^T[ACGT]AGC
BpmI 112 CTGGAG CTGGAG(16/14)
Bpu1102I 186 GCTGAGC GC^T[ACGT]AGC
BsaBI 345 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 66 GTCCC GGGAC(10/14)
BsrBI 234 CCGCTC CCGCTC(-3/-3)
BssHII 216 GCGCGC G^CGCGC
CelII 186 GCTGAGC GC^T[ACGT]AGC
DsaI 221 CCACGG C^C[GA][CT]GG
EcoT14I 86 CCAAGG C^C[AT][AT]GG
HaeII 203 AGCGCC [GA]GCGC^[CT]
MamI 345 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 362 TGGCCA TGG^CCA
MscI 362 TGGCCA TGG^CCA
MspA1I 184 CAGCTG C[AC]G^C[GT]G
NaeI 212 GCCGGC GCC^GGC
NciI 324 CCCGG CC^[GC]GG
NgoMIV 212 GCCGGC G^CCGGC
PflMI 390 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 184 CAGCTG CAG^CTG
RsaI 231 GTAC GT^AC
SacI 295 GAGCTC GAGCT^C
SgrAI 211 CGCCGGCG C[GA]^CCGG[CT]G
StyI 86 CCAAGG C^C[AT][AT]GG
TaqI 347 TCGA T^CGA
TfiI 28 GATTC G^A[AT]TC
Tsp509I 305 AATT ^AATT
TthHB8I 347 TCGA T^CGA
Van91I 390 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 12 TCCGGA T^CCGGA
60 TCCGGA
AspHI 91 GTGCTC G[AT]GC[AT]^C
295 GAGCTC
BmyI 91 GTGCTC G[AGT]GC[ACT]^C
295 GAGCTC
BsaWI 12 TCCGGA [AT]^CCGG[AT]
60 TCCGGA
BseAI 12 TCCGGA T^CCGGA
60 TCCGGA
BseRI 124 GAGGAG GAGGAG(10/8)
196 GAGGAG
BsiHKAI 91 GTGCTC G[AT]GC[AT]^C
295 GAGCTC
Bsp1286I 91 GTGCTC G[AGT]GC[ACT]^C
295 GAGCTC
BspEI 12 TCCGGA T^CCGGA
60 TCCGGA
BsrFI 117 GCCGGT [GA]^CCGG[CT]
212 GCCGGC
BstF5I 37 CATCC GGATG(2/0)
261 CATCC
BstYI 57 GGATCC [GA]^GATC[CT]
350 AGATCC
Cfr10I 117 GCCGGT [GA]^CCGG[CT]
212 GCCGGC
DdeI 7 CTGAG C^T[ACGT]AG
187 CTGAG
EaeI 224 CGGCCA [CT]^GGCC[GA]
362 TGGCCA
FokI 37 CATCC GGATG(9/13)
261 CATCC
MaeII 392 ACGT A^CGT
401 ACGT
MflI 57 GGATCC [GA]^GATC[CT]
350 AGATCC
MroI 12 TCCGGA T^CCGGA
60 TCCGGA
MslI 84 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
261 CATCCGCGTG
NlaIV 57 GGATCC GG[ACGT]^[ACGT]CC
114 GGAGCC
PleI 9 GAGTC GAGTC(4/5)
64 GAGTC
SfaNI 3 GATGC GCATC(5/9)
260 GCATC
XhoII 57 GGATCC [GA]^GATC[CT]
350 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbvI BsaJI BsiYI BslI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DpnII EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MnlI MspI MvaI
MvnI MwoI NdeII Sau3AI Sau96I
ScrFI TseI