Restriction map for
pF1KB9376
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeII MamI MflI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TthHB8I
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AspHI 546 GAGCAC G[AT]GC[AT]^C
BalI 422 TGGCCA TGG^CCA
BanI 591 GGCACC G^G[CT][GA]CC
BclI 243 TGATCA T^GATCA
BpmI 1019 CTGGAG CTGGAG(16/14)
BsgI 268 GTGCAG GTGCAG(16/14)
BsiHKAI 546 GAGCAC G[AT]GC[AT]^C
BsmAI 340 GTCTC GTCTC(1/5)
BsmBI 339 CGTCTC CGTCTC(1/5)
BsmFI 254 GTCCC GGGAC(10/14)
Bsp1407I 516 TGTACA T^GTACA
BsrGI 516 TGTACA T^GTACA
Cac8I 699 GCTGGC GC[ACGT]^[ACGT]GC
EaeI 422 TGGCCA [CT]^GGCC[GA]
EarI 16 CTCTTC CTCTTC(1/4)
EcoT22I 80 ATGCAT ATGCA^T
FbaI 243 TGATCA T^GATCA
HgaI 338 GCGTC GACGC(5/10)
HindIII 521 AAGCTT A^AGCTT
HphI 361 GGTGA GGTGA(8/7)
Ksp632I 16 CTCTTC CTCTTC(1/4)
MfeI 329 CAATTG C^AATTG
MluNI 422 TGGCCA TGG^CCA
MscI 422 TGGCCA TGG^CCA
MunI 329 CAATTG C^AATTG
MwoI 539 GCACAGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 80 ATGCAT ATGCA^T
NspI 905 GCATGT [GA]CATG^[CT]
PflMI 652 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 715 CTGCAG CTGCA^G
SspBI 516 TGTACA T^GTACA
TfiI 103 GATTC G^A[AT]TC
Van91I 652 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 843 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
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AciI 12 CCGC CCGC(-3/-1)
72 GCGG
Asp700I 662 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1079 GAAAGTATTC
AspI 334 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
847 GACTTTGTC
AvaII 253 GGTCC G^G[AT]CC
277 GGACC
BanII 30 GGGCTC G[GA]GC[CT]^C
37 GGGCTC
BcnI 21 CCCGG CC^[GC]GG
28 CCGGG
BfaI 55 CTAG C^TAG
844 CTAG
BsrDI 936 CATTGC GCAATG(2/0)
683 GCAATG
Cfr13I 253 GGTCC G^G[ACGT]CC
277 GGACC
DpnII 244 GATC ^GATC
1045 GATC
DsaI 204 CCATGG C^C[GA][CT]GG
707 CCATGG
EcoRI 398 GAATTC G^AATTC
431 GAATTC
HaeIII 423 GGCC GG^CC
1152 GGCC
HapII 22 CCGG C^CGG
28 CCGG
HpaII 22 CCGG C^CGG
28 CCGG
MaeI 55 CTAG C^TAG
844 CTAG
MboI 244 GATC ^GATC
1045 GATC
MnlI 15 CCTC CCTC(7/6)
1119 CCTC
MslI 606 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
900 CATCAGCATG
MspI 22 CCGG C^CGG
28 CCGG
NciI 21 CCCGG CC^[GC]GG
28 CCGGG
NcoI 204 CCATGG C^CATGG
707 CCATGG
NdeII 244 GATC ^GATC
1045 GATC
PflFI 334 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
847 GACTTTGTC
PleI 818 GACTC GAGTC(4/5)
1111 GACTC
Sau3AI 244 GATC ^GATC
1045 GATC
Sau96I 253 GGTCC G^G[ACGT]CC
277 GGACC
SfcI 715 CTGCAG C^T[GA][CT]AG
1088 CTACAG
Tsp45I 214 GTGAC ^GT[GC]AC
1069 GTGAC
Tth111I 334 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
847 GACTTTGTC
XmnI 662 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1079 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
DdeI EcoRII EcoT14I Fnu4HI FokI
HinfI ItaI MaeIII MboII MseI
MspA1I MvaI NlaIII NlaIV PvuII
RsaI ScrFI SfaNI StyI Tru9I
TseI Tsp509I TspRI