Restriction map for
pF1KB0069
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BcgI BcnI BglI BlnI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstZ17I
Cfr10I CpoI Eam1105I Eco47III EcoNI
FseI FspI HapII Hin1I HpaII
KasI KpnI KspI MamI MluI
MluNI MroI MscI MspI NaeI
NarI NciI NgoMIV NheI NruI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I Tth111I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 1097 CGCG CG^CG
AvaI 4639 CTCGAG C^[CT]CG[GA]G
BbrPI 2561 CACGTG CAC^GTG
BciVI 682 GTATCC GTATCC(6/5)
BlpI 6985 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 6985 GCTAAGC GC^T[ACGT]AGC
BsgI 915 CTGCAC GTGCAG(16/14)
BsiEI 40 CGGCCG CG[GA][CT]^CG
BsmBI 1093 GAGACG CGTCTC(1/5)
BsoBI 4639 CTCGAG C^[CT]CG[GA]G
BspDI 211 ATCGAT AT^CGAT
BspMI 742 GCAGGT ACCTGC(4/8)
BsrDI 1108 GCAATG GCAATG(2/0)
BstAPI 6697 GCATCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1203 GGTGACC G^GT[ACGT]ACC
BstPI 1203 GGTGACC G^GT[ACGT]ACC
BstUI 1097 CGCG CG^CG
BstXI 7687 CCAGGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 6985 GCTAAGC GC^T[ACGT]AGC
CfoI 737 GCGC GCG^C
ClaI 211 ATCGAT AT^CGAT
DraIII 1505 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 100 GACAATAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 40 CGGCCG C^GGCCG
EclXI 40 CGGCCG C^GGCCG
Eco52I 40 CGGCCG C^GGCCG
EcoO65I 1203 GGTGACC G^GT[ACGT]ACC
EcoRV 7179 GATATC GAT^ATC
HaeII 736 GGCGCT [GA]GCGC^[CT]
HgaI 1095 GACGC GACGC(5/10)
HhaI 737 GCGC GCG^C
HinP1I 737 GCGC G^CGC
MvnI 1097 CGCG CG^CG
NcoI 6125 CCATGG C^CATGG
NotI 39 GCGGCCGC GC^GGCCGC
PaeR7I 4639 CTCGAG C^TCGAG
PmaCI 2561 CACGTG CAC^GTG
PmlI 2561 CACGTG CAC^GTG
PshAI 4824 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2230 GAGCTC GAGCT^C
SapI 2227 GAAGAGC GCTCTTC(1/4)
SspI 3151 AATATT AAT^ATT
SwaI 5534 ATTTAAAT ATTT^AAAT
XhoI 4639 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AflII 4816 CTTAAG C^TTAAG
5398 CTTAAG
AocI 4995 CCTTAGG CC^T[ACGT]AGG
7706 CCTCAGG
BanI 1268 GGTGCC G^G[CT][GA]CC
6703 GGTGCC
BanII 1100 GGGCTC G[GA]GC[CT]^C
2230 GAGCTC
BbvCI 7198 CCTCAGC CCTCAGC(-5/-2)
2199 GCTGAGG
BfrI 4816 CTTAAG C^TTAAG
5398 CTTAAG
BsmFI 795 GGGAC GGGAC(10/14)
2759 GGGAC
Bsp1407I 3360 TGTACA T^GTACA
7245 TGTACA
BsrGI 3360 TGTACA T^GTACA
7245 TGTACA
Bsu36I 4995 CCTTAGG CC^T[ACGT]AGG
7706 CCTCAGG
DsaI 17 CCGTGG C^C[GA][CT]GG
6125 CCATGG
EaeI 20 TGGCCG [CT]^GGCC[GA]
40 CGGCCG
Eco81I 4995 CCTTAGG CC^T[ACGT]AGG
7706 CCTCAGG
FauI 1098 GCGGG CCCGC(4/6)
2142 GCGGG
MfeI 1123 CAATTG C^AATTG
3127 CAATTG
MslI 160 CATGGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5203 CACTCAAATG
MunI 1123 CAATTG C^AATTG
3127 CAATTG
NdeI 2401 CATATG CA^TATG
2911 CATATG
NspI 5105 ACATGC [GA]CATG^[CT]
5264 ACATGC
SspBI 3360 TGTACA T^GTACA
7245 TGTACA
StuI 3612 AGGCCT AGG^CCT
7635 AGGCCT
XbaI 1617 TCTAGA T^CTAGA
5929 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BbsI BbvI
BclI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HpaI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
PflMI PleI PpuMI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI