Restriction map for
pF1KB7849
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbvCI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsgI BsiEI BsiYI BslI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI Sau96I SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 1085 GGTACC G^GTACC
AlwNI 590 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1085 GGTACC G^GTACC
BanI 1085 GGTACC G^G[CT][GA]CC
BcgI 443 GCAAAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 466 AGATCT A^GATCT
BsaJI 125 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 1123 ACCGGA [AT]^CCGG[AT]
BsiWI 1388 CGTACG C^GTACG
BsmBI 1010 CGTCTC CGTCTC(1/5)
BspMI 106 ACCTGC ACCTGC(4/8)
BstBI 1268 TTCGAA TT^CGAA
BstF5I 1168 GGATG GGATG(2/0)
BstXI 43 CCAGAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 466 AGATCT [GA]^GATC[CT]
EcoT14I 125 CCTTGG C^C[AT][AT]GG
EcoT22I 719 ATGCAT ATGCA^T
FauI 1208 GCGGG CCCGC(4/6)
FokI 1168 GGATG GGATG(9/13)
HaeIII 1161 GGCC GG^CC
HapII 1124 CCGG C^CGG
HindIII 656 AAGCTT A^AGCTT
HpaII 1124 CCGG C^CGG
KpnI 1085 GGTACC GGTAC^C
MflI 466 AGATCT [GA]^GATC[CT]
MslI 1110 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1124 CCGG C^CGG
MwoI 518 GCCTCTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 719 ATGCAT ATGCA^T
NspV 1268 TTCGAA TT^CGAA
SbfI 107 CCTGCAGG CCTGCA^GG
ScaI 1430 AGTACT AGT^ACT
SfuI 1268 TTCGAA TT^CGAA
Sse8387I 107 CCTGCAGG CCTGCA^GG
StuI 1160 AGGCCT AGG^CCT
StyI 125 CCTTGG C^C[AT][AT]GG
XhoII 466 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 63 GGATC GGATC(4/5)
157 GGATC
BbsI 738 GAAGAC GAAGAC(2/6)
1187 GAAGAC
BbvI 512 GCAGC GCAGC(8/12)
515 GCTGC
BpuAI 738 GAAGAC GAAGAC(2/6)
1187 GAAGAC
BsrDI 56 CATTGC GCAATG(2/0)
982 CATTGC
BssKI 236 CCTGG ^CC[ACGT]GG
576 CCAGG
BstNI 236 CCTGG CC^[AT]GG
576 CCAGG
DraIII 432 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1454 CACACAGTG
EarI 1013 CTCTTC CTCTTC(1/4)
46 GAAGAG
EcoRII 236 CCTGG ^CC[AT]GG
576 CCAGG
HgaI 948 GACGC GACGC(5/10)
1284 GACGC
HphI 1212 GGTGA GGTGA(8/7)
38 TCACC
Ksp632I 1013 CTCTTC CTCTTC(1/4)
46 GAAGAG
MvaI 236 CCTGG CC^[AT]GG
576 CCAGG
PleI 326 GACTC GAGTC(4/5)
580 GACTC
PstI 108 CTGCAG CTGCA^G
510 CTGCAG
ScrFI 236 CCTGG CC^[ACGT]GG
576 CCAGG
SmlI 366 CTTGAG C^T[CT][GA]AG
582 CTCAAG
TseI 512 GCAGC G^C[AT]GC
515 GCTGC
Tsp45I 598 GTGAC ^GT[GC]AC
1340 GTCAC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AflIII AluI Alw44I
ApaLI Asp700I AspHI BbrPI BmyI
BpmI BsaAI BsaI BseRI BsiHKAI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrI Cac8I DdeI DpnII Fnu4HI
HinfI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I NdeII NlaIII
NlaIV NspI PmaCI PmlI PvuII
RsaI Sau3AI SfaNI SfcI TaqI
TfiI Tsp509I TspRI TthHB8I XmnI