Restriction map for
pF1KA0296
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# NO CUT ENZYMES ( 77 enzymes) #
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Acc65I AccII AccIII AclI AflIII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI BamHI BciVI BclI
BsaBI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI HindIII
KpnI KspI MamI MluI MroI
MvnI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SfuI
SnaBI SpeI SspBI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 4995 GACGTC GACGT^C
AccI 1198 GTCTAC GT^[AC][GT]AC
AcsI 3036 GAATTC [GA]^AATT[CT]
AflII 5155 CTTAAG C^TTAAG
Alw44I 1040 GTGCAC G^TGCAC
Aor51HI 2847 AGCGCT AGC^GCT
ApaLI 1040 GTGCAC G^TGCAC
ApoI 3036 GAATTC [GA]^AATT[CT]
Asp700I 3792 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1520 TGCGCA TGC^GCA
AvrII 1840 CCTAGG C^CTAGG
BbeI 2699 GGCGCC GGCGC^C
BbrPI 1528 CACGTG CAC^GTG
BcgI 4666 CGACACTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5155 CTTAAG C^TTAAG
BglII 492 AGATCT A^GATCT
BlnI 1840 CCTAGG C^CTAGG
BsaWI 5374 TCCGGT [AT]^CCGG[AT]
BsmI 2481 GCATTC GAATGC(1/-1)
BsrDI 1540 CATTGC GCAATG(2/0)
DrdI 1824 GACCACAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2847 AGCGCT AGC^GCT
EcoNI 4906 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3036 GAATTC G^AATTC
FspI 1520 TGCGCA TGC^GCA
HincII 190 GTTAAC GT[CT]^[GA]AC
HindII 190 GTTAAC GT[CT]^[GA]AC
HpaI 190 GTTAAC GTT^AAC
KasI 2699 GGCGCC G^GCGCC
MfeI 4255 CAATTG C^AATTG
MunI 4255 CAATTG C^AATTG
NarI 2699 GGCGCC GG^CGCC
NdeI 277 CATATG CA^TATG
PmaCI 1528 CACGTG CAC^GTG
PmlI 1528 CACGTG CAC^GTG
PshAI 1191 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4809 CCTGCAGG CCTGCA^GG
SexAI 4892 ACCAGGT A^CC[AT]GGT
SfiI 4186 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2699 GGCGCC GGC^GCC
SphI 861 GCATGC GCATG^C
Sse8387I 4809 CCTGCAGG CCTGCA^GG
XmnI 3792 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AocI 295 CCTGAGG CC^T[ACGT]AGG
538 CCTGAGG
ApaI 3334 GGGCCC GGGCC^C
3376 GGGCCC
BsaAI 483 TACGTG [CT]AC^GT[GA]
1528 CACGTG
BsmBI 358 GAGACG CGTCTC(1/5)
3964 GAGACG
BssSI 208 CACGAG CACGAG(-5/-1)
3961 CACGAG
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
538 CCTGAGG
DraIII 5037 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5412 CACACAGTG
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
538 CCTGAGG
SacI 64 GAGCTC GAGCT^C
1066 GAGCTC
Tsp509I 3037 AATT ^AATT
4256 AATT
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# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SgrAI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI