Restriction map for
pF1KB7879
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII BalI BamHI
BanII BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRV FbaI
FseI FspI HapII HgaI HincII
HindII HindIII HpaI HpaII KpnI
Ksp632I KspI MamI MluI MluNI
MroI MscI MspI NaeI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 517 CGCG CG^CG
AcyI 20 GGCGCC G[GA]^CG[CT]C
Asp700I 693 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 796 GGACC G^G[AT]CC
AvrII 244 CCTAGG C^CTAGG
BbeI 20 GGCGCC GGCGC^C
BlnI 244 CCTAGG C^CTAGG
BmyI 36 GGGCAC G[AGT]GC[ACT]^C
BsaHI 20 GGCGCC G[GA]^CG[CT]C
Bsp1286I 36 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 216 TGTACA T^GTACA
BspHI 1359 TCATGA T^CATGA
BspMI 250 GCAGGT ACCTGC(4/8)
BsrGI 216 TGTACA T^GTACA
BstEII 1009 GGTAACC G^GT[ACGT]ACC
BstPI 1009 GGTAACC G^GT[ACGT]ACC
BstUI 517 CGCG CG^CG
BstYI 1258 AGATCC [GA]^GATC[CT]
DraIII 1429 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 244 CCTAGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1009 GGTAACC G^GT[ACGT]ACC
EcoRI 1619 GAATTC G^AATTC
EcoT14I 244 CCTAGG C^C[AT][AT]GG
EcoT22I 598 ATGCAT ATGCA^T
FauI 902 GCGGG CCCGC(4/6)
HaeII 20 GGCGCC [GA]GCGC^[CT]
Hin1I 20 GGCGCC G[GA]^CG[CT]C
KasI 20 GGCGCC G^GCGCC
MfeI 1308 CAATTG C^AATTG
MflI 1258 AGATCC [GA]^GATC[CT]
MspA1I 5 CAGCGG C[AC]G^C[GT]G
MunI 1308 CAATTG C^AATTG
MvnI 517 CGCG CG^CG
NarI 20 GGCGCC GG^CGCC
NdeI 1388 CATATG CA^TATG
NsiI 598 ATGCAT ATGCA^T
NspI 370 GCATGT [GA]CATG^[CT]
PpuMI 795 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1359 TCATGA T^CATGA
SfoI 20 GGCGCC GGC^GCC
SspBI 216 TGTACA T^GTACA
StyI 244 CCTAGG C^C[AT][AT]GG
TaqI 834 TCGA T^CGA
TfiI 1452 GATTC G^A[AT]TC
TthHB8I 834 TCGA T^CGA
XhoII 1258 AGATCC [GA]^GATC[CT]
XmnI 693 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwI 1259 GATCC GGATC(4/5)
175 GGATC
AlwNI 5 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
349 CAGAAGCTG
BanI 20 GGCGCC G^G[CT][GA]CC
192 GGCACC
BsgI 723 GTGCAG GTGCAG(16/14)
1563 GTGCAG
BsmI 1477 GAATGC GAATGC(1/-1)
1645 GAATGC
CfoI 21 GCGC GCG^C
1516 GCGC
HhaI 21 GCGC GCG^C
1516 GCGC
HinP1I 21 GCGC G^CGC
1516 GCGC
HphI 273 TCACC GGTGA(8/7)
1188 TCACC
MaeII 480 ACGT A^CGT
533 ACGT
MwoI 411 GCACCAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
929 GCCTTATTAGC
SfaNI 129 GATGC GCATC(5/9)
463 GCATC
SfcI 441 CTACAG C^T[GA][CT]AG
572 CTGTAG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI StuI Tru9I TseI Tsp45I
Tsp509I TspRI