##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI SphI Sse8387I SspI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AciI 98 CCGC CCGC(-3/-1)
AflIII 1321 ACATGT A^C[GA][CT]GT
AlwI 315 GGATC GGATC(4/5)
ApaI 107 GGGCCC GGGCC^C
BbsI 556 GTCTTC GAAGAC(2/6)
BclI 495 TGATCA T^GATCA
BcnI 464 CCGGG CC^[GC]GG
BglI 100 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 90 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 90 GCTGAGC GC^T[ACGT]AGC
BpuAI 556 GTCTTC GAAGAC(2/6)
BsaAI 777 TACGTA [CT]AC^GT[GA]
BsaI 150 GGTCTC GGTCTC(1/5)
BseRI 768 CTCCTC GAGGAG(10/8)
BsgI 930 GTGCAG GTGCAG(16/14)
BsmI 817 GAATGC GAATGC(1/-1)
Bsp1407I 113 TGTACA T^GTACA
BspLU11I 1321 ACATGT A^CATGT
BspMI 14 ACCTGC ACCTGC(4/8)
BsrGI 113 TGTACA T^GTACA
BstEII 39 GGTGACC G^GT[ACGT]ACC
BstPI 39 GGTGACC G^GT[ACGT]ACC
CelII 90 GCTGAGC GC^T[ACGT]AGC
DraI 898 TTTAAA TTT^AAA
DsaI 23 CCATGG C^C[GA][CT]GG
EcoO65I 39 GGTGACC G^GT[ACGT]ACC
EcoT14I 23 CCATGG C^C[AT][AT]GG
FauI 97 CCCGC CCCGC(4/6)
FbaI 495 TGATCA T^GATCA
HapII 464 CCGG C^CGG
HpaII 464 CCGG C^CGG
MspA1I 88 CAGCTG C[AC]G^C[GT]G
MspI 464 CCGG C^CGG
NciI 464 CCGGG CC^[GC]GG
NcoI 23 CCATGG C^CATGG
NdeI 1329 CATATG CA^TATG
NheI 367 GCTAGC G^CTAGC
NspI 1321 ACATGT [GA]CATG^[CT]
PpuMI 168 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 88 CAGCTG CAG^CTG
SexAI 146 ACCTGGT A^CC[AT]GGT
SfcI 138 CTACAG C^T[GA][CT]AG
SnaBI 777 TACGTA TAC^GTA
SspBI 113 TGTACA T^GTACA
StyI 23 CCATGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
Asp700I 1086 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1170 GAAAGCTTTC
BbvI 87 GCAGC GCAGC(8/12)
411 GCAGC
BsmAI 1545 GAGAC GTCTC(1/5)
151 GTCTC
BsrDI 384 GCAATG GCAATG(2/0)
989 GCAATG
Fnu4HI 87 GCAGC GC^[ACGT]GC
411 GCAGC
ItaI 87 GCAGC GC^[ACGT]GC
411 GCAGC
NlaIII 24 CATG CATG^
1322 CATG
PleI 161 GACTC GAGTC(4/5)
1449 GACTC
SacI 477 GAGCTC GAGCT^C
765 GAGCTC
StuI 752 AGGCCT AGG^CCT
1088 AGGCCT
TseI 87 GCAGC G^C[AT]GC
411 GCAGC
XmnI 1086 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1170 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
AvaII BanII BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRI EcoRII FokI
HaeIII HindIII HinfI HphI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I Tsp45I Tsp509I
TspRI