Restriction map for
pF1KB7876
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I FauI FbaI Fnu4HI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII HphI ItaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI TseI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 21 enzymes) #
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AccII 1703 CGCG CG^CG
AciI 419 GCGG CCGC(-3/-1)
AfaI 69 GTAC GT^AC
AvaII 481 GGACC G^G[AT]CC
BseRI 357 CTCCTC GAGGAG(10/8)
BspHI 1128 TCATGA T^CATGA
BstUI 1703 CGCG CG^CG
Cfr13I 481 GGACC G^G[ACGT]CC
DsaI 471 CCGTGG C^C[GA][CT]GG
HaeIII 24 GGCC GG^CC
HindIII 1769 AAGCTT A^AGCTT
MaeIII 1838 GTAAC ^GT[ACGT]AC
MvnI 1703 CGCG CG^CG
NruI 1702 TCGCGA TCG^CGA
RcaI 1128 TCATGA T^CATGA
RsaI 69 GTAC GT^AC
SacI 1449 GAGCTC GAGCT^C
SapI 770 GCTCTTC GCTCTTC(1/4)
Sau96I 481 GGACC G^G[ACGT]CC
SfaNI 1305 GATGC GCATC(5/9)
SfcI 29 CTATAG C^T[GA][CT]AG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwI 1433 GATCC GGATC(4/5)
1609 GGATC
AspHI 236 GTGCTC G[AT]GC[AT]^C
1449 GAGCTC
BfaI 608 CTAG C^TAG
893 CTAG
BglII 1189 AGATCT A^GATCT
1525 AGATCT
BsaJI 155 CCCAGG C^C[ACGT][ACGT]GG
471 CCGTGG
BsiHKAI 236 GTGCTC G[AT]GC[AT]^C
1449 GAGCTC
BsiYI 26 CCTCTATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
887 CCCACTCTAGG
BslI 26 CCTCTATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
887 CCCACTCTAGG
BsmAI 108 GAGAC GTCTC(1/5)
353 GTCTC
BsmFI 857 GTCCC GGGAC(10/14)
1781 GTCCC
BssKI 21 CCTGG ^CC[ACGT]GG
156 CCAGG
BstBI 617 TTCGAA TT^CGAA
1793 TTCGAA
BstNI 21 CCTGG CC^[AT]GG
156 CCAGG
Cac8I 1418 GCAAGC GC[ACGT]^[ACGT]GC
1502 GCAAGC
EarI 771 CTCTTC CTCTTC(1/4)
1315 GAAGAG
EcoRII 21 CCTGG ^CC[AT]GG
156 CCAGG
Ksp632I 771 CTCTTC CTCTTC(1/4)
1315 GAAGAG
MaeI 608 CTAG C^TAG
893 CTAG
MseI 251 TTAA T^TAA
1103 TTAA
MvaI 21 CCTGG CC^[AT]GG
156 CCAGG
NlaIV 17 GGAACC GG[ACGT]^[ACGT]CC
159 GGTTCC
NspV 617 TTCGAA TT^CGAA
1793 TTCGAA
ScrFI 21 CCTGG CC^[ACGT]GG
156 CCAGG
SfuI 617 TTCGAA TT^CGAA
1793 TTCGAA
Tru9I 251 TTAA T^TAA
1103 TTAA
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BanII
BmyI BpmI BsmI Bsp1286I BsrDI
BsrI BstF5I BstYI DdeI DpnII
EcoRV EcoT22I FokI HinfI MboI
MboII MflI MnlI MslI NdeI
NdeII NlaIII NsiI NspI PleI
Sau3AI TaqI Tsp509I TspRI TthHB8I
XhoII XmnI