Restriction map for
pF1KB7830
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BseAI BsgI
BsiEI BsiHKAI BsiWI Bsp1407I BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FbaI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AciI 15 GCGG CCGC(-3/-1)
AcyI 241 GGCGTC G[GA]^CG[CT]C
AflII 1338 CTTAAG C^TTAAG
AlwNI 313 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 71 CCCGAG C^[CT]CG[GA]G
AvaII 1809 GGACC G^G[AT]CC
BalI 1593 TGGCCA TGG^CCA
BanII 160 GGGCTC G[GA]GC[CT]^C
BfrI 1338 CTTAAG C^TTAAG
BmyI 160 GGGCTC G[AGT]GC[ACT]^C
BsaAI 1691 CACGTA [CT]AC^GT[GA]
BsaHI 241 GGCGTC G[GA]^CG[CT]C
BsaI 150 GGTCTC GGTCTC(1/5)
BsaWI 1213 ACCGGA [AT]^CCGG[AT]
BseRI 246 CTCCTC GAGGAG(10/8)
BsiYI 873 CCATACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 873 CCATACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 243 CGTCTC CGTCTC(1/5)
BsmFI 315 GGGAC GGGAC(10/14)
BsmI 1457 GAATGC GAATGC(1/-1)
BsoBI 71 CCCGAG C^[CT]CG[GA]G
Bsp1286I 160 GGGCTC G[AGT]GC[ACT]^C
BspDI 1565 ATCGAT AT^CGAT
BsrDI 1235 GCAATG GCAATG(2/0)
BssSI 1366 CACGAG CACGAG(-5/-1)
Cac8I 90 GCTGGC GC[ACGT]^[ACGT]GC
ClaI 1565 ATCGAT AT^CGAT
EaeI 1593 TGGCCA [CT]^GGCC[GA]
EcoRV 1584 GATATC GAT^ATC
EcoT14I 349 CCTTGG C^C[AT][AT]GG
EcoT22I 1459 ATGCAT ATGCA^T
FauI 15 GCGGG CCCGC(4/6)
HgaI 242 GCGTC GACGC(5/10)
Hin1I 241 GGCGTC G[GA]^CG[CT]C
HincII 285 GTCAAC GT[CT]^[GA]AC
HindII 285 GTCAAC GT[CT]^[GA]AC
HphI 1533 TCACC GGTGA(8/7)
MluNI 1593 TGGCCA TGG^CCA
MscI 1593 TGGCCA TGG^CCA
MspA1I 13 CAGCGG C[AC]G^C[GT]G
MwoI 519 GCTTATTAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 810 CATATG CA^TATG
NsiI 1459 ATGCAT ATGCA^T
PflMI 873 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 301 GACTC GAGTC(4/5)
PpuMI 1808 AGGACCC [GA]G^G[AT]CC[CT]
SapI 261 GAAGAGC GCTCTTC(1/4)
SexAI 146 ACCTGGT A^CC[AT]GGT
SmlI 1338 CTTAAG C^T[CT][GA]AG
StuI 933 AGGCCT AGG^CCT
StyI 349 CCTTGG C^C[AT][AT]GG
Tsp45I 36 GTCAC ^GT[GC]AC
Van91I 873 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1045 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1660 ACATGT A^C[GA][CT]GT
1744 ACATGT
BcnI 117 CCGGG CC^[GC]GG
988 CCGGG
BsmAI 151 GTCTC GTCTC(1/5)
244 GTCTC
BspLU11I 1660 ACATGT A^CATGT
1744 ACATGT
BstYI 210 AGATCC [GA]^GATC[CT]
331 AGATCC
DraI 1144 TTTAAA TTT^AAA
1648 TTTAAA
DraII 25 AGGCCCC [GA]G^G[ACGT]CC[CT]
1808 AGGACCC
EcoO109I 25 AGGCCCC [GA]G^G[ACGT]CC[CT]
1808 AGGACCC
MaeII 43 ACGT A^CGT
1692 ACGT
MflI 210 AGATCC [GA]^GATC[CT]
331 AGATCC
NciI 117 CCGGG CC^[GC]GG
988 CCGGG
NspI 1660 ACATGT [GA]CATG^[CT]
1744 ACATGT
TfiI 1286 GAATC G^A[AT]TC
1626 GATTC
XhoII 210 AGATCC [GA]^GATC[CT]
331 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbvI BfaI BpmI BsaJI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EarI EcoRI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XmnI